One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2 [0.03%]
SOX10、OLIG2和NKX6.2基因诱导的人成纤维细胞向少突胶质细胞样细胞的重编程
Konstantina Chanoumidou,Benjamín Hernández-Rodríguez,Farina Windener et al.
Konstantina Chanoumidou et al.
Limited access to human oligodendrocytes impairs better understanding of oligodendrocyte pathology in myelin diseases. Here, we describe a method to robustly convert human fibroblasts directly into oligodendrocyte-like cells (dc-hiOLs), whi...
Stem cell-based models of embryos: The need for improved naming conventions [0.03%]
基于干细胞的胚胎模型：改进命名规范的需求
Kirstin R W Matthews,Daniel S Wagner,Aryeh Warmflash
Kirstin R W Matthews
Stem cell-based models of embryos are known by various names, with different naming conventions, leading to confusion regarding their composition and potential. We propose the need for a general term for the field to promote public engageme...
HIC1 Represses Atoh1 Transcription and Hair Cell Differentiation in the Cochlea [0.03%]
HIC1通过抑制耳蜗Atoh1转录和分化来抑制毛细胞的生成
Dunia Abdul-Aziz,Nicolai Hathiramani,Lauren Phung et al.
Dunia Abdul-Aziz et al.
Across species, expression of the basic helix-loop-helix transcription factor ATOH1 promotes differentiation of cochlear supporting cells to sensory hair cells required for hearing. In mammals, this process is limited to development, wherea...
Reconciling Flux Experiments for Quantitative Modeling of Normal and Malignant Hematopoietic Stem/Progenitor Dynamics [0.03%]
造血干细胞/祖细胞定量建模的增流实验协调研究（正常和恶性）
Munetomo Takahashi,Melania Barile,Richard H Chapple et al.
Munetomo Takahashi et al.
Hematopoiesis serves as a paradigm for how homeostasis is maintained within hierarchically organized cell populations. However, important questions remain as to the contribution of hematopoietic stem cells (HSCs) toward maintaining steady s...
Inaccessible LCG Promoters Act as Safeguards to Restrict T Cell Development to Appropriate Notch Signaling Environments [0.03%]
不可接近的LCG启动子充当限制T细胞发育至适当Notch信号环境的安全保障
Suzanne Furuyama,Qian Vicky Wu,Barbara Varnum-Finney et al.
Suzanne Furuyama et al.
T cell development is restricted to the thymus and is dependent on high levels of Notch signaling induced within the thymic microenvironment. To understand Notch function in thymic restriction, we investigated the basis for target gene sele...
Species-Specific Relationships between DNA and Chromatin Properties of CpG Islands in Embryonic Stem Cells and Differentiated Cells [0.03%]
胚胎干细胞和分化细胞中CpG岛DNA与染色质属性的物种特异性关系
Justin Langerman,David Lopez,Matteo Pellegrini et al.
Justin Langerman et al.
CpG islands often exhibit low DNA methylation, high histone H3 lysine 4 trimethylation, low nucleosome density, and high DNase I hypersensitivity, yet the rules by which CpG islands are sensed remain poorly understood. In this study, we fir...
Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds [0.03%]
高效地使用 lentiviral vectors 对培养的原始生殖细胞进行基因组改造以生成转基因鸣鸟
Ivana Gessara,Falk Dittrich,Moritz Hertel et al.
Ivana Gessara et al.
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic devel...
Integrated Collection of Stem Cell Bank Data, a Data Portal for Standardized Stem Cell Information [0.03%]
整合的干细胞库数据收集以及标准干细胞信息的数据平台
Ying Chen,Kunie Sakurai,Sumihiro Maeda et al.
Ying Chen et al.
The past decade has witnessed an extremely rapid increase in the number of newly established stem cell lines. However, due to the lack of a standardized format, data exchange among stem cell line resources has been challenging, and no syste...
Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein [0.03%]
通过CRISPR-Cas9/sgRNA核糖核蛋白预复合物避免细胞抑制性RNA，实现高效单链寡脱氧核苷酸介导的靶向基因编辑
Akihiro Kagita,Mandy S Y Lung,Huaigeng Xu et al.
Akihiro Kagita et al.
Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knocki...
Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer [0.03%]
比较分析匹配患者来源的类器官揭示结直肠癌转移病灶中OLFＭ4相关簇减少
Takuya Okamoto,David duVerle,Katsuyuki Yaginuma et al.
Takuya Okamoto et al.
Metastasis is the major cause of cancer-related death, but whether metastatic lesions exhibit the same cellular composition as primary tumors has yet to be elucidated. To investigate the cellular heterogeneity of metastatic colorectal cance...
Comparative Study
Stem cell reports. 2021 Apr 13;16(4):954-967. DOI:10.1016/j.stemcr.2021.02.012 2021