Lesch-Nyhan disease causes impaired energy metabolism and reduced developmental potential in midbrain dopaminergic cells [0.03%]
Lesch-Nyhan病导致中脑多巴胺能细胞能量代谢受损和发育潜能降低
Scott Bell,Vincent McCarty,Huashan Peng et al.
Scott Bell et al.
Mutations in HPRT1, a gene encoding a rate-limiting enzyme for purine salvage, cause Lesch-Nyhan disease which is characterized by self-injury and motor impairments. We leveraged stem cell and genetic engineering technologies to model the d...
Ethics and regulatory considerations for the clinical translation of somatic cell human epigenetic editing [0.03%]
体细胞人类表观遗传编辑的临床转化的伦理和监管考量
Nikolajs Zeps,Tamra Lysaght,Ruth Chadwick et al.
Nikolajs Zeps et al.
Altering the human epigenome with gene-editing technology in attempt to treat a variety of diseases and conditions seems scientifically feasible. We explore some of the ethical and regulatory issues related to the clinical translation of hu...
Human pluripotent stem cell-derived eosinophils reveal potent cytotoxicity against solid tumors [0.03%]
人多能干细胞来源的嗜酸性粒细胞对实体瘤具有强大的细胞毒性
Weifeng Lai,Huangfan Xie,Yuting Liu et al.
Weifeng Lai et al.
Eosinophils are attractive innate immune cells to use to potentiate T cell antitumor efficacy because they are capable of infiltrating tumors at early stages and modulating the tumor microenvironment. However, the limited number of function...
Ran Zheng,Ting Geng,Dan-Ya Wu et al.
Ran Zheng et al.
Human extended pluripotent stem cells (EPSCs), with bidirectional chimeric ability to contribute to both embryonic and extraembryonic lineages, can be obtained and maintained by converting conventional pluripotent stem cells using chemicals...
Uncoupling key determinants of hematopoietic stem cell engraftment through cell-specific and temporally controlled recipient conditioning [0.03%]
通过细胞特异性及时间控制性受体准备解离造血干细胞植入的关键决定因素
Natsumi Miharada,Anna Rydström,Justyna Rak et al.
Natsumi Miharada et al.
Hematopoietic stem cells (HSCs) are typically characterized by transplantation into irradiated hosts in a highly perturbed microenvironment. Here, we show that selective and temporally controlled depletion of resident HSCs through genetic d...
Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics [0.03%]
高效生成具有中脑特征的多巴胺能神经细胞
Yi Han Ng,Soham Chanda,Justyna A Janas et al.
Yi Han Ng et al.
The differentiation of pluripotent stem cells can be accomplished by sequential activation of signaling pathways or through transcription factor programming. Multistep differentiation imitates embryonic development to obtain authentic cell ...
Reprogramming astrocytes to motor neurons by activation of endogenous Ngn2 and Isl1 [0.03%]
通过激活内源性Ngn2和Isl1重新编程星形胶质细胞为运动神经元
Meiling Zhou,Xiaoqing Tao,Ming Sui et al.
Meiling Zhou et al.
Central nervous system injury and neurodegenerative diseases cause irreversible loss of neurons. Overexpression of exogenous specific transcription factors can reprogram somatic cells into functional neurons for regeneration and functional ...
Differentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source medium [0.03%]
一种人诱导多能干细胞分化为巨噬细胞的方法，使用了一种明确的、不含血清的和开源培养基
Alun Vaughan-Jackson,Szymon Stodolak,Kourosh H Ebrahimi et al.
Alun Vaughan-Jackson et al.
Human induced pluripotent stem cells (iPSCs) and macrophages derived from them are increasingly popular tools for research into both infectious and degenerative diseases. However, as the field strives for greater modeling accuracy, it is be...
Lawrence E Bates,Mariana R P Alves,José C R Silva
Lawrence E Bates
The pluripotency factor OCT4 is essential for the maintenance of naive pluripotent stem cells in vitro and in vivo. However, the specific role of OCT4 in this process remains unknown. Here, we developed a rapid protein-level OCT4 depletion ...
Sequential regulation of hemogenic fate and hematopoietic stem and progenitor cell formation from arterial endothelium by Ezh1/2 [0.03%]
Ezh1/2依次调节动脉内皮向造血细胞的命运决定和造血干细胞及祖细胞的形成
Rebecca A Soto,Mohamad Ali T Najia,Mariam Hachimi et al.
Rebecca A Soto et al.
Across species, hematopoietic stem and progenitor cells (HSPCs) arise during embryogenesis from a specialized arterial population, termed hemogenic endothelium. Here, we describe a mechanistic role for the epigenetic regulator, Enhancer of ...