Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty [0.03%]
人多能干细胞衍生的角膜内皮细胞作为角膜移植中供体角膜内皮的替代品
Muhammad Ali,Shahid Y Khan,John D Gottsch et al.
Muhammad Ali et al.
Here, we evaluate the efficacy of cryopreserved human embryonic stem cell (hESC)-derived corneal endothelial cells (CECs) to form a functional monolayer of corneal endothelium (CE) in rabbits and monkeys. We injected cryopreserved hESC-deri...
Hsiu-Chuan Lin,Zhisong He,Sebastian Ebert et al.
Hsiu-Chuan Lin et al.
Human neurons engineered from induced pluripotent stem cells (iPSCs) through neurogenin 2 (NGN2) overexpression are widely used to study neuronal differentiation mechanisms and to model neurological diseases. However, the differentiation pa...
The relevance of mitochondrial DNA variants fluctuation during reprogramming and neuronal differentiation of human iPSCs [0.03%]
人诱导多能干细胞重编程和神经分化过程中线粒体DNA变异的相关性分析
Flavia Palombo,Camille Peron,Leonardo Caporali et al.
Flavia Palombo et al.
The generation of inducible pluripotent stem cells (iPSCs) is a revolutionary technique allowing production of pluripotent patient-specific cell lines used for disease modeling, drug screening, and cell therapy. Integrity of nuclear DNA (nD...
Inclusivity and diversity: Integrating international perspectives on stem cell challenges and potential [0.03%]
包容性和多样性：整合国际视角下的干细胞挑战与潜力
Robin Fears,Hidenori Akutsu,Lara Theresa Alentajan-Aleta et al.
Robin Fears et al.
Regenerative medicine has great potential. The pace of scientific advance is exciting and the medical opportunities for regeneration and repair may be transformative. However, concerns continue to grow, relating to problems caused both by u...
Sonlicromanol improves neuronal network dysfunction and transcriptome changes linked to m.3243A>G heteroplasmy in iPSC-derived neurons [0.03%]
SONL改善诱导多能干细胞来源的神经元中线粒体DNA异质性引起的神经网络功能障碍和转录组改变
Teun M Klein Gunnewiek,Anouk H A Verboven,Iris Pelgrim et al.
Teun M Klein Gunnewiek et al.
Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is often caused by an adenine to guanine variant at m.3243 (m.3243A>G) of the MT-TL1 gene. To understand how this pathogenic variant affects the nervous syst...
Juli Bollinger,Elizabeth May,Debra Mathews et al.
Juli Bollinger et al.
Organoid research is enhancing understanding of human development and diseases as well as aiding in medication development and selection, raising hopes for even more future therapeutic options. Nevertheless, this work raises important ethic...
Human neuronal networks on micro-electrode arrays are a highly robust tool to study disease-specific genotype-phenotype correlations in vitro [0.03%]
微电极阵列上的人神经网络是体外研究疾病特异性基因型表型相关性的强大工具
Britt Mossink,Anouk H A Verboven,Eline J H van Hugte et al.
Britt Mossink et al.
Micro-electrode arrays (MEAs) are increasingly used to characterize neuronal network activity of human induced pluripotent stem cell (hiPSC)-derived neurons. Despite their gain in popularity, MEA recordings from hiPSC-derived neuronal netwo...
A BMP4-p38 MAPK signaling axis controls ISL1 protein stability and activity during cardiogenesis [0.03%]
BMP4-p38MAPK信号轴在心脏发育过程中控制ISL1蛋白稳定性和活性
Yanyan Jing,Yonggang Ren,Hagen Roland Witzel et al.
Yanyan Jing et al.
During development, cells respond rapidly to intra- and intercellular signals, which induce signaling cascades regulating the activity of transcription factors at the transcriptional and/or post-translational level. The transcription factor...
Developing human pluripotent stem cell-based cerebral organoids with a controllable microglia ratio for modeling brain development and pathology [0.03%]
可控小胶质细胞比率的多能干细胞来源脑器官芯片用于开发和模拟大脑生理及病理学研究
Ranjie Xu,Andrew J Boreland,Xiaoxi Li et al.
Ranjie Xu et al.
Microglia play critical roles in brain development, homeostasis, and disease. Microglia in animal models cannot accurately model human microglia due to notable transcriptomic and functional differences between human and other animal microgl...
Massimo Mastrangeli,Janny van den Eijnden-van Raaij
Massimo Mastrangeli
Organ-on-chip (OoC) technology is thriving thanks to stem cells availability and international OoC programs. Concerted standardization, qualification, and independent testing of devices are needed to coherently develop OoC technology furthe...