Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [0.03%]
TBX3的丢失可增强来自人多能干细胞的胰腺祖细胞的生成能力
Somdutta Mukherjee,Deborah L French,Paul Gadue
Somdutta Mukherjee
Tbx3 has been identified as a regulator of liver development in the mouse, but its function in human liver development remains unknown. TBX3 mutant human pluripotent stem cell (PSC) lines were generated using CRISPR/Cas9 genome editing. TBX...
Cardiomyocytes recruit monocytes upon SARS-CoV-2 infection by secreting CCL2 [0.03%]
心肌细胞在SARS-CoV-2感染过程中通过分泌CCL2招募单核细胞
Liuliu Yang,Benjamin E Nilsson-Payant,Yuling Han et al.
Liuliu Yang et al.
Published Erratum
Stem cell reports. 2021 Oct 12;16(10):2565. DOI:10.1016/j.stemcr.2021.09.008 2021
Cell cycle heterogeneity directs spontaneous 2C state entry and exit in mouse embryonic stem cells [0.03%]
细胞周期异质性指导小鼠胚胎干细胞自发进入和退出2C状态
Yuqing Zhu,Chen Cheng,Lang Chen et al.
Yuqing Zhu et al.
Mouse embryonic stem cells (ESCs) show cell-to-cell heterogeneity. A small number of two-cell-like cells (2CLCs) marked by endogenous retrovirus activation emerge spontaneously. The 2CLCs are unstable and they are prone to transiting back t...
Large-scale analysis of imprinting in naive human pluripotent stem cells reveals recurrent aberrations and a potential link to FGF signaling [0.03%]
naive人多能干细胞中印迹的大型分析揭示了反复异常以及与FGF信号的潜在联系
Gal Keshet,Nissim Benvenisty
Gal Keshet
Genomic imprinting is a parent-of-origin dependent monoallelic expression of genes. Previous studies showed that conversion of primed human pluripotent stem cells (hPSCs) into naive pluripotency is accompanied by genome-wide loss of methyla...
Peter Y Zhao,Sunjong Ji,Paula Anne Newman-Casey et al.
Peter Y Zhao et al.
A wide variety of stem cell-derived therapies are under development for the treatment of retinal degeneration. In order to better understand patient perspectives about these therapies, we assessed risk tolerance using an in-person survey of...
Isolation of neural stem and oligodendrocyte progenitor cells from the brain of live rats [0.03%]
从活的大脑中分离神经干细胞和少突胶质细胞前体细胞
Freyja McClenahan,Christina Dimitriou,Christos Koutsakis et al.
Freyja McClenahan et al.
Postnatal brain neural stem and progenitor cells (NSPCs) cluster in anatomically inaccessible stem cell niches, such as the subependymal zone (SEZ). Here, we describe a method for the isolation of NSPCs from live animals, which we term "mil...
iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome [0.03%]
iPSC培养扩增排除了线粒体基因组中潜在的可操作突变
Maike Kosanke,Colin Davenport,Monika Szepes et al.
Maike Kosanke et al.
Therapeutic application of induced pluripotent stem cell (iPSC) derivatives requires comprehensive assessment of the integrity of their nuclear and mitochondrial DNA (mtDNA) to exclude oncogenic potential and functional deficits. It is unkn...
Insertion/deletion and microsatellite alteration profiles in induced pluripotent stem cells [0.03%]
诱导性多能干细胞中的插入/删除和微卫星改变谱型分析
Satoshi Kamimura,Tomo Suga,Yuko Hoki et al.
Satoshi Kamimura et al.
We here demonstrate that microsatellite (MS) alterations are elevated in both mouse and human induced pluripotent stem cells (iPSCs), but importantly we have now identified a type of human iPSC in which these alterations are considerably re...
Programmatic introduction of parenchymal cell types into blood vessel organoids [0.03%]
血血管器官中实质细胞类型的程序性引入
Amir Dailamy,Udit Parekh,Dhruva Katrekar et al.
Amir Dailamy et al.
Pluripotent stem cell-derived organoids have transformed our ability to recreate complex three-dimensional models of human tissue. However, the directed differentiation methods used to create them do not afford the ability to introduce cros...
CRISPR/Cas9/AAV9-mediated in vivo editing identifies MYC regulation of 3D genome in skeletal muscle stem cell [0.03%]
CRISPR/Cas9/AAV9介导的体内编辑鉴定MYC在骨骼肌干细胞中对3D基因组的调控作用
Liangqiang He,Yingzhe Ding,Yu Zhao et al.
Liangqiang He et al.
Skeletal muscle satellite cells (SCs) are stem cells responsible for muscle development and regeneration. Although CRISPR/Cas9 has been widely used, its application in endogenous SCs remains elusive. Here, we generate mice expressing Cas9 i...