A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals [0.03%]
来自临床特征明显、健康的多样化人类个体的诱导性多能干细胞库
Christoph Schaniel,Priyanka Dhanan,Bin Hu et al.
Christoph Schaniel et al.
A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotyp...
William Zofkie,Sheryl M Southard,Thomas Braun et al.
William Zofkie et al.
Skeletal muscle stem cells, i.e., satellite cells (SCs), are the essential source of new myonuclei for skeletal muscle regeneration following injury or chronic degenerative myopathies. Both SC number and regenerative capacity diminish durin...
PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs [0.03%]
PCSK9调节hiPSC中的NODAL信号通路及细胞增殖
Meryl Roudaut,Salam Idriss,Amandine Caillaud et al.
Meryl Roudaut et al.
Proprotein convertase subtilisin kexin type 9 (PCSK9) is a key regulator of low-density lipoprotein (LDL) cholesterol metabolism and the target of lipid-lowering drugs. PCSK9 is mainly expressed in hepatocytes. Here, we show that PCSK9 is h...
CITED2 coordinates key hematopoietic regulatory pathways to maintain the HSC pool in both steady-state hematopoiesis and transplantation [0.03%]
CITED2协调关键的造血调节通路以维持稳态和移植过程中的HSC池
Hannah Lawson,Louie N van de Lagemaat,Melania Barile et al.
Hannah Lawson et al.
Hematopoietic stem cells (HSCs) reside at the apex of the hematopoietic differentiation hierarchy and sustain multilineage hematopoiesis. Here, we show that the transcriptional regulator CITED2 is essential for life-long HSC maintenance. Wh...
p53 inactivation unmasks histone methylation-independent WDR5 functions that drive self-renewal and differentiation of pluripotent stem cells [0.03%]
p53失活揭示了驱动多能干细胞自我更新和分化的不依赖组蛋白甲基化的WDR5功能
Qiang Li,Yuanhao Huang,Jing Xu et al.
Qiang Li et al.
p53 alterations occur during culture of pluripotent stem cells (PSCs), but the significance of these events on epigenetic control of PSC fate determination remains poorly understood. Wdr5 deletion in p53-null (DKO) mouse ESCs (mESCs) leads ...
Plating human iPSC lines on micropatterned substrates reveals role for ITGB1 nsSNV in endoderm formation [0.03%]
在微观结构基底上培养人类iPSC细胞系揭示ITGB1 nsSNV 在内胚层形成中的作用
Alice Vickers,Mukul Tewary,Anna Laddach et al.
Alice Vickers et al.
Quantitative analysis of human induced pluripotent stem cell (iPSC) lines from healthy donors is a powerful tool for uncovering the relationship between genetic variants and cellular behavior. We previously identified rare, deleterious non-...
A new platform for high-throughput therapy testing on iPSC-derived lung progenitor cells from cystic fibrosis patients [0.03%]
用于囊性纤维化患者诱导多能干细胞来源的肺前体细胞的高通量治疗测试的新平台
Jia Xin Jiang,Leigh Wellhauser,Onofrio Laselva et al.
Jia Xin Jiang et al.
For those people with cystic fibrosis carrying rare CFTR mutations not responding to currently available therapies, there is an unmet need for relevant tissue models for therapy development. Here, we describe a new testing platform that emp...
Fumarylacetoacetate hydrolase gene as a knockout target for hepatic chimerism and donor liver production [0.03%]
鼠李糖水解酶基因作为敲除靶标在肝嵌合体及供体肝脏生产中的应用
Ellen L Larson,Dong Jin Joo,Erek D Nelson et al.
Ellen L Larson et al.
A reliable source of human hepatocytes and transplantable livers is needed. Interspecies embryo complementation, which involves implanting donor human stem cells into early morula/blastocyst stage animal embryos, is an emerging solution to ...
CALR frameshift mutations in MPN patient-derived iPSCs accelerate maturation of megakaryocytes [0.03%]
MPN患者来源的ipscc中calr移码突变加速巨核细胞的成熟过程
Kathrin Olschok,Lijuan Han,Marcelo A S de Toledo et al.
Kathrin Olschok et al.
Calreticulin (CALR) mutations are driver mutations in myeloproliferative neoplasms (MPNs), leading to activation of the thrombopoietin receptor and causing abnormal megakaryopoiesis. Here, we generated patient-derived CALRins5- or CALRdel52...
PBX1-directed stem cell transcriptional program drives tumor progression in myeloproliferative neoplasm [0.03%]
在骨髓增生性肿瘤中，由PBX1指导的干细胞转录程序驱动肿瘤进展
Sharon Muggeo,Laura Crisafulli,Paolo Uva et al.
Sharon Muggeo et al.
PBX1 regulates the balance between self-renewal and differentiation of hematopoietic stem cells and maintains proto-oncogenic transcriptional pathways in early progenitors. Its increased expression was found in myeloproliferative neoplasm (...