APOE2, E3, and E4 differentially modulate cellular homeostasis, cholesterol metabolism, and inflammatory response in isogenic iPSC-derived astrocytes [0.03%]
APOE2、E3和E4同种基因源iPSC衍生星形胶质细胞的细胞稳态、胆固醇代谢及炎性反应的调节作用不同
Sherida M de Leeuw,Aron W T Kirschner,Karina Lindner et al.
Sherida M de Leeuw et al.
The apolipoprotein E4 (APOE4) variant is the strongest genetic risk factor for Alzheimer disease (AD), while the APOE2 allele is protective. A major question is how different APOE genotypes affect the physiology of astrocytes, the main APOE...
Cell position within human pluripotent stem cell colonies determines apical specialization via an actin cytoskeleton-based mechanism [0.03%]
人多能干细胞聚集物中的细胞位置通过基于肌动蛋白细胞骨架的机制决定顶侧特殊化
Youngju Kim,Hwanseok Jang,Kyubin Seo et al.
Youngju Kim et al.
Human pluripotent stem cells (hPSCs) grow as colonies with epithelial-like features including cell polarity and position-dependent features that contribute to symmetry breaking during development. Our study provides evidence that hPSC colon...
Differentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source medium [0.03%]
一种用于将人类诱导多能干细胞分化为真正的巨噬细胞的定义条件、无血清和开放源培养基
Alun Vaughan-Jackson,Szymon Stodolak,Kourosh H Ebrahimi et al.
Alun Vaughan-Jackson et al.
Published Erratum
Stem cell reports. 2021 Dec 14;16(12):3093. DOI:10.1016/j.stemcr.2021.11.010 2021
Development of human iPSC-derived quiescent hepatic stellate cell-like cells for drug discovery and in vitro disease modeling [0.03%]
人类iPSC来源的静止态肝星状细胞样细胞的开发在药物发现和体外疾病建模中的应用
Yuta Koui,Misao Himeno,Yusuke Mori et al.
Yuta Koui et al.
Hepatic stellate cells (HSCs) play a central role in the progression of liver fibrosis by producing extracellular matrices. The development of drugs to suppress liver fibrosis has been hampered by the lack of human quiescent HSCs (qHSCs) an...
Modulating adult neurogenesis affects synaptic plasticity and cognitive functions in mouse models of Alzheimer's disease [0.03%]
调节成体神经发生影响阿尔茨海默病小鼠模型的突触可塑性和认知功能
Xiaoqin Zhang,Xiaojie Wei,Yufei Mei et al.
Xiaoqin Zhang et al.
New neurons are abnormal in the adult hippocampus of Alzheimer's disease (AD) mouse models. The effects of modulating adult neurogenesis on AD pathogenesis differ from study to study. We reported recently that ablation of adult neural stem ...
Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells [0.03%]
机器人高通量生物制造和人类多能干细胞的功能分化
Carlos A Tristan,Pinar Ormanoglu,Jaroslav Slamecka et al.
Carlos A Tristan et al.
Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive,...
Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs [0.03%]
p53和CDKN1A的沉默建立了来自人类iPSC的可持续永久性巨核细胞祖细胞
Masamitsu Sone,Sou Nakamura,Sachiko Umeda et al.
Masamitsu Sone et al.
Platelet transfusions are critical for severe thrombocytopenia but depend on blood donors. The shortage of donors and the potential of universal HLA-null platelet products have stimulated research on the ex vivo differentiation of human plu...
Rescuing human fetal tissue research in the United States: A call for additional regulatory reform [0.03%]
在美国拯救人类胎儿组织研究：对额外监管改革的呼吁
Katherine E MacDuffie,Insoo Hyun,Michaela M Krogen et al.
Katherine E MacDuffie et al.
Research using human fetal tissue has saved millions of lives through vaccines and other advances, but was markedly restricted by federal regulations in 2019. Although the restrictions were partially reversed in 2021, additional regulatory ...
Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts [0.03%]
患者成纤维细胞的高效基础编辑和重编程同步进行
Sami Jalil,Timo Keskinen,Rocío Maldonado et al.
Sami Jalil et al.
Human induced pluripotent stem cells (hiPSCs) allow in vitro study of genetic diseases and hold potential for personalized stem cell therapy. Gene editing, precisely modifying specifically targeted loci, represents a valuable tool for diffe...
Cell autonomous TGFβ signaling is essential for stem/progenitor cell recruitment into degenerative tendons [0.03%]
细胞自主的TGFβ信号对于干细胞/祖细胞招募到退变的肌腱中是必不可少的
Guak-Kim Tan,Brian A Pryce,Anna Stabio et al.
Guak-Kim Tan et al.
Understanding cell recruitment in damaged tendons is critical for improvements in regenerative therapy. We recently reported that targeted disruption of transforming growth factor beta (TGFβ) type II receptor in the tendon cell lineage (Tg...