CRISPR/Cas9 editing of directly reprogrammed myogenic progenitors restores dystrophin expression in a mouse model of muscular dystrophy [0.03%]
CRISPR/Cas9编辑直接重编程的肌源性祖细胞在肌肉萎缩小鼠模型中恢复了dystrophin的表达
Seraina A Domenig,Nicola Bundschuh,Ajda Lenardič et al.
Seraina A Domenig et al.
Genetic mutations in dystrophin manifest in Duchenne muscular dystrophy (DMD), the most commonly inherited muscle disease. Here, we report on reprogramming of fibroblasts from two DMD mouse models into induced myogenic progenitor cells (iMP...
RNF43/ZNRF3 negatively regulates taste tissue homeostasis and positively regulates dorsal lingual epithelial tissue homeostasis [0.03%]
RNF43/ZNRF3负向调控味觉组织稳态而正向调控舌背粘膜上皮组织稳态
Chanyi Lu,Xiaoli Lin,Jumpei Yamashita et al.
Chanyi Lu et al.
Taste bud cells are renewed throughout life in a process requiring innervation. Recently, we reported that R-spondin substitutes for neuronal input for taste cell regeneration. R-spondin amplifies WNT signaling by interacting with stem-cell...
Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation [0.03%]
制作神经元，易如反掌：Neurogenin-2在神经元分化中的应用
Amy J Hulme,Simon Maksour,Mitchell St-Clair Glover et al.
Amy J Hulme et al.
Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Ex...
A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants [0.03%]
一种新颖的双因子单突触TRIO示踪法 用于评估人类胚胎干细胞来源多巴胺能细胞移植后与宿主神经环路的整合情况
Patrick Aldrin-Kirk,Malin Åkerblom,Tiago Cardoso et al.
Patrick Aldrin-Kirk et al.
Transplantation in Parkinson's disease using human embryonic stem cell (hESC)-derived dopaminergic (DA) neurons is a promising future treatment option. However, many of the mechanisms that govern their differentiation, maturation, and integ...
Arun Sharma,Rachel A Clemens,Orquidea Garcia et al.
Arun Sharma et al.
Research in low Earth orbit (LEO) has become more accessible. The 2020 Biomanufacturing in Space Symposium reviewed space-based regenerative medicine research and discussed leveraging LEO to advance biomanufacturing for regenerative medicin...
Differential repression of Otx2 underlies the capacity of NANOG and ESRRB to induce germline entry [0.03%]
NANOG和ESRRB诱导生殖系进入的能力在于Otx2的不同抑制作用
Matúš Vojtek,Jingchao Zhang,Juanjuan Sun et al.
Matúš Vojtek et al.
Primordial germ cells (PGCs) arise from cells of the post-implantation epiblast in response to cytokine signaling. PGC development can be recapitulated in vitro by differentiating epiblast-like cells (EpiLCs) into PGC-like cells (PGCLCs) th...
An episomal DNA vector platform for the persistent genetic modification of pluripotent stem cells and their differentiated progeny [0.03%]
一种用于持久性遗传修饰多能干细胞及其分化子细胞的染色体外DNA载体平台
Alicia Roig-Merino,Manuela Urban,Matthias Bozza et al.
Alicia Roig-Merino et al.
The genetic modification of stem cells (SCs) is typically achieved using integrating vectors, whose potential integrative genotoxicity and propensity for epigenetic silencing during differentiation limit their application. The genetic modif...
A functional hiPSC-cortical neuron differentiation and maturation model and its application to neurological disorders [0.03%]
人 iPSC 的皮层神经元功能分化和成熟模型及其在神经系统疾病中的应用研究
Kaveena Autar,Xiufang Guo,John W Rumsey et al.
Kaveena Autar et al.
The maturation and functional characteristics of human induced pluripotent stem cell (hiPSC)-cortical neurons has not been fully documented. This study developed a phenotypic model of hiPSC-derived cortical neurons, characterized their matu...
Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming [0.03%]
体细胞重编程过程中X染色体失活状态早期逆转激活基因的集群效应
Shiho Aizawa,Ken Nishimura,Gonzalo Seminario Mondejar et al.
Shiho Aizawa et al.
Reprogramming of murine female somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by X chromosome reactivation (XCR), by which the inactive X chromosome (Xi) in female somatic cells becomes reactivated. However, how Xi i...
Haploid mouse germ cell precursors from embryonic stem cells reveal Xist activation from a single X chromosome [0.03%]
来源于胚胎干细胞的小鼠单倍体生殖细胞前体揭示了从单一X染色体的Xist活化
Eishi Aizawa,Corinne Kaufmann,Sarah Sting et al.
Eishi Aizawa et al.
Mammalian haploid cells have applications for genetic screening and substituting gametic genomes. Here, we characterize a culture system for obtaining haploid primordial germ cell-like cells (PGCLCs) from haploid mouse embryonic stem cells ...