Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers [0.03%]
一种维持胚系特征的人类原始生殖细胞样细胞无血清和饲养层的同质培养体系
Mutsumi Kobayashi,Misato Kobayashi,Junko Odajima et al.
Mutsumi Kobayashi et al.
In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. ...
Srebf1c preserves hematopoietic stem cell function and survival as a switch of mitochondrial metabolism [0.03%]
SREBF1C通过切换线粒体代谢来维持造血干细胞功能和存活
Yukai Lu,Zihao Zhang,Song Wang et al.
Yukai Lu et al.
Mitochondria are fundamental but complex determinants for hematopoietic stem cell (HSC) maintenance. However, the factors involved in the regulation of mitochondrial metabolism in HSCs and the underlying mechanisms have not been fully eluci...
FOXG1 dose tunes cell proliferation dynamics in human forebrain progenitor cells [0.03%]
FOXG1剂量调节人类前脑祖细胞增殖动力学
Nuwan C Hettige,Huashan Peng,Hanrong Wu et al.
Nuwan C Hettige et al.
Heterozygous loss-of-function mutations in Forkhead box G1 (FOXG1), a uniquely brain-expressed gene, cause microcephaly, seizures, and severe intellectual disability, whereas increased FOXG1 expression is frequently observed in glioblastoma...
Deriving waveform parameters from calcium transients in human iPSC-derived cardiomyocytes to predict cardiac activity with machine learning [0.03%]
基于人多能干细胞源心肌细胞的钙瞬变提取心电图波形参数及其机器学习预测研究
Hongbin Yang,Will Stebbeds,Jo Francis et al.
Hongbin Yang et al.
Human induced pluripotent stem cell-derived cardiomyocytes have been established to detect dynamic calcium transients by fast kinetic fluorescence assays that provide insights into specific aspects of clinical cardiac activity. However, the...
Genetic and epigenetic determinants of reactivation of Mecp2 and the inactive X chromosome in neural stem cells [0.03%]
神经干细胞中Mecp2和失活X染色体再激活的遗传和表观遗传决定因素
H Mira-Bontenbal,B Tan,C Gontan et al.
H Mira-Bontenbal et al.
Rett syndrome may be treated by reactivating the silent copy of Mecp2 from the inactive X chromosome in female cells. Most studies that model Mecp2 reactivation have used mouse fibroblasts rather than neural cells, which would be critical f...
YAP signaling in horizontal basal cells promotes the regeneration of olfactory epithelium after injury [0.03%]
YAP信号通路通过水平基底细胞促进损伤后嗅觉上皮的再生
Qian Wu,Xingxing Xu,Xuemeng Miao et al.
Qian Wu et al.
The horizontal basal cells (HBCs) of olfactory epithelium (OE) serve as reservoirs for stem cells during OE regeneration, through proliferation and differentiation, which is important in recovery of olfactory function. However, the molecula...
Leveraging interacting signaling pathways to robustly improve the quality and yield of human pluripotent stem cell-derived hepatoblasts and hepatocytes [0.03%]
利用相互作用的信号通路稳健改善人多能干细胞来源肝前体细胞和肝细胞的质量和产量
Claudia Raggi,Marie-Agnès M&#x;Callum,Quang Toan Pham et al.
Claudia Raggi et al.
Pluripotent stem cell (PSC)-derived hepatocyte-like cells (HLCs) have shown great potential as an alternative to primary human hepatocytes (PHHs) for in vitro modeling. Several differentiation protocols have been described to direct PSCs to...
Homozygous ALS-linked FUS P525L mutations cell- autonomously perturb transcriptome profile and chemoreceptor signaling in human iPSC microglia [0.03%]
同源ALS相关FUS P525L突变自主扰乱人类iPSC小胶质细胞转录组轮廓和化学感受器信号转导
Sze Yen Kerk,Yu Bai,Janell Smith et al.
Sze Yen Kerk et al.
Amyotrophic lateral sclerosis is a fatal disease pathologically typified by motor and cortical neurodegeneration as well as microgliosis. The FUS P525L mutation is highly penetrant and causes ALS cases with earlier disease onset and more ag...
Developmental thyroid disruption permanently affects the neuroglial output in the murine subventricular zone [0.03%]
甲状腺发育性干扰永久影响小鼠侧脑室区神经胶质细胞的产生
Pieter Vancamp,Karine Le Blay,Lucile Butruille et al.
Pieter Vancamp et al.
Neural stem cells (NSCs) in the adult brain are a source of neural cells for brain injury repair. We investigated whether their capacity to generate new neurons and glia is determined by thyroid hormone (TH) during development because serum...
Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin [0.03%]
laminin 411通过多个信号轴介导内皮细胞分化，这些信号轴汇聚于β-catenin蛋白质
Mikayla L Hall,Sophie Givens,Natasha Santosh et al.
Mikayla L Hall et al.
The extracellular matrix (ECM) provides essential cues to promote endothelial specification during tissue development in vivo; correspondingly, ECM is considered essential for endothelial differentiation outside of the body. However, system...