Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells [0.03%]
人小肠和结肠上皮干细胞单层的高效转基因和同源重组敲入体系
Keith A Breau,Meryem T Ok,Ismael Gomez-Martinez et al.
Keith A Breau et al.
Two-dimensional (2D) cultures of intestinal and colonic epithelium can be generated using human intestinal stem cells (hISCs) derived from primary tissue sources. These 2D cultures are emerging as attractive and versatile alternatives to th...
Iron accumulation induces oxidative stress, while depressing inflammatory polarization in human iPSC-derived microglia [0.03%]
铁沉积诱导氧化应激，抑制人类iPSC来源的小胶质细胞的炎症极化
Boyd Kenkhuis,Michelle van Eekeren,David A Parfitt et al.
Boyd Kenkhuis et al.
Iron accumulation in microglia has been observed in Alzheimer's disease and other neurodegenerative disorders and is thought to contribute to disease progression through various mechanisms, including neuroinflammation. To study this interac...
Differentiation of brain and retinal organoids from confluent cultures of pluripotent stem cells connected by nerve-like axonal projections of optic origin [0.03%]
通过视神经样轴突连接的多能干细胞共聚焦培养分化脑和视网膜类器官
Milan Fernando,Scott Lee,Jesse R Wark et al.
Milan Fernando et al.
Advances in the study of neurological conditions have been possible because of pluripotent stem cell technologies and organoids. Studies have described the generation of neural ectoderm-derived retinal and brain structures from pluripotent ...
Imogen R Brooks,Cristina M Garrone,Caoimhe Kerins et al.
Imogen R Brooks et al.
Induced pluripotent stem cells (iPSCs) are valuable in disease modeling because of their potential to expand and differentiate into virtually any cell type and recapitulate key aspects of human biology. Functional genomics are genome-wide s...
Sleep deprivation induces corneal epithelial progenitor cell over-expansion through disruption of redox homeostasis in the tear film [0.03%]
睡眠剥夺通过破坏泪膜氧化还原稳态诱导角膜上皮干细胞过度增殖
Sanming Li,Liying Tang,Jing Zhou et al.
Sanming Li et al.
Sleep deficiency, a common public health problem, causes ocular discomfort and affects ocular surface health. However, the underlying mechanism remains unclear. Herein, we identified that short-term sleep deprivation (SD) resulted in hyperp...
Investigating the feasibility and ethical implications of phenotypic screening using stem cell-derived tissue models to detect and manage disease [0.03%]
利用干细胞衍生组织模型进行表型筛选以检测和管理疾病的可行性和伦理影响的研究
Alexander R Harris,Mary Jean Walker,Frederic Gilbert et al.
Alexander R Harris et al.
Stem-cell-derived tissue models generated from sick people are being used to understand human development and disease, drug development, and drug screening. However, it is possible to detect disease phenotypes before a patient displays symp...
Prepubertal androgen signaling is required to establish male fat distribution [0.03%]
青春期前的雄激素信号传导是建立男性脂肪分布所必需的
Zachary L Sebo,Matthew S Rodeheffer
Zachary L Sebo
Fat distribution is sexually dimorphic and is associated with metabolic disease risk. It is unknown if prepubertal sex-hormone signaling influences adult fat distribution. Here, we show that karyotypically male androgen-insensitive mice exh...
Argonaute proteins regulate a specific network of genes through KLF4 in mouse embryonic stem cells [0.03%]
Argonaute蛋白通过KLF4调控小鼠胚胎干细胞中的特定基因网络
Madlen Müller,Moritz Schaefer,Tara Fäh et al.
Madlen Müller et al.
The Argonaute proteins (AGOs) are well known for their role in post-transcriptional gene silencing in the microRNA (miRNA) pathway. Here we show that in mouse embryonic stem cells, AGO1&2 serve additional functions that go beyond the miRNA ...
Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation [0.03%]
在纤连蛋白或无基质聚合物上进行的人胰腺前体细胞的长期无饲养层培养促进β细胞分化
Akiko Nakamura,Yan Fung Wong,Andrea Venturato et al.
Akiko Nakamura et al.
With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While in vivo pancreatic progenitors...
The SARS-CoV-2 receptor ACE2 is expressed in mouse pericytes but not endothelial cells: Implications for COVID-19 vascular research [0.03%]
SARS-CoV-2受体ACE2在小鼠周细胞中表达，但不在内皮细胞中表达：对COVID-19血管研究的影响
Lars Muhl,Liqun He,Ying Sun et al.
Lars Muhl et al.
Humanized mouse models and mouse-adapted SARS-CoV-2 virus are increasingly used to study COVID-19 pathogenesis, so it is important to learn where the SARS-CoV-2 receptor ACE2 is expressed. Here we mapped ACE2 expression during mouse postnat...