Direct visualization of the transition status during neural differentiation by dual-fluorescent reporter human pluripotent stem cells [0.03%]
双荧光报告人多能干细胞直接观察神经分化过程中的过渡状态
Gwanghyun Park,Minkyung Shin,Wonyoung Lee et al.
Gwanghyun Park et al.
Human induced pluripotent stem cells (hiPSCs) can differentiate into neurons and glia via neural progenitor cells and are widely used for neurogenic studies. However, directly visualizing the transition status during the neural differentiat...
Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation [0.03%]
血管内皮特异性GATA-3表达对造血干细胞生成的必需性研究
Nada Zaidan,Leslie Nitsche,Evangelia Diamanti et al.
Nada Zaidan et al.
To generate sufficient numbers of transplantable hematopoietic stem cells (HSCs) in vitro, a detailed understanding of how this process takes place in vivo is essential. The endothelial-to-hematopoietic transition (EHT), which culminates in...
UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells [0.03%]
Uhrf1与snrna相互作用并调节小鼠生精干细胞的可变剪接事件
Shumin Zhou,Juan Dong,Mengneng Xiong et al.
Shumin Zhou et al.
Life-long male fertility relies on exquisite homeostasis and the development of spermatogonial stem cells (SSCs); however, the underlying molecular genetic and epigenetic regulation in this equilibrium process remains unclear. Here, we docu...
Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation [0.03%]
用于更可靠和可重复的人多能干细胞来源的小肠类器官生成的冷冻保存的中后肠内胚层的聚集
Amy L Pitstick,Holly M Poling,Nambirajan Sundaram et al.
Amy L Pitstick et al.
A major technical limitation hindering the widespread adoption of human pluripotent stem cell (hPSC)-derived gastrointestinal (GI) organoid technologies is the need for de novo hPSC differentiation and dependence on spontaneous morphogenesi...
Systemic immunosuppression promotes survival and integration of subretinally implanted human ESC-derived photoreceptor precursors in dogs [0.03%]
全身免疫抑制可促进人胚胎干细胞来源的感光细胞前体在犬视网膜下的存活和整合
Ana Ripolles-Garcia,Natalia Dolgova,M Joseph Phillips et al.
Ana Ripolles-Garcia et al.
Regenerative therapies aimed at replacing photoreceptors are a promising approach for the treatment of otherwise incurable causes of blindness. However, such therapies still face significant hurdles, including the need to improve subretinal...
Mechanical force-promoted osteoclastic differentiation via periodontal ligament stem cell exosomal protein ANXA3 [0.03%]
牙周韧带干细胞源外泌体蛋白ANXA3介导的机械力促破骨细胞分化作用及机制研究
Hua-Ming Huang,Chun-Shan Han,Sheng-Jie Cui et al.
Hua-Ming Huang et al.
Exosomes play a critical role in intracellular communication. The biogenesis and function of exosomes are regulated by multiple biochemical factors. In the present study, we find that mechanical force promotes the biogenesis of exosomes der...
Characterizing arrhythmia using machine learning analysis of Ca2+ cycling in human cardiomyocytes [0.03%]
使用机器学习分析人类心肌细胞中的Ca2+循环特征以表征心律失常事件
Jeremy K S Pang,Sabrina Chia,Jinqiu Zhang et al.
Jeremy K S Pang et al.
Accurate modeling of the heart electrophysiology to predict arrhythmia susceptibility remains a challenge. Current electrophysiological analyses are hypothesis-driven models drawing conclusions from changes in a small subset of electrophysi...
Autism NPCs from both idiopathic and CNV 16p11.2 deletion patients exhibit dysregulation of proliferation and mitogenic responses [0.03%]
自闭症NPCs源自16p11. 2缺失的染色体重排患者增殖和促有丝分裂反应失调
Robert Connacher,Madeline Williams,Smrithi Prem et al.
Robert Connacher et al.
Published Erratum
Stem cell reports. 2022 Jul 12;17(7):1786. DOI:10.1016/j.stemcr.2022.06.007 2022
Recent advances in "sickle and niche" research - Tribute to Dr. Paul S Frenette [0.03%]
近期“镰刀与生态位”研究进展——致保尔·弗伦内特博士
Lidiane S Torres,Noboru Asada,Mitchell J Weiss et al.
Lidiane S Torres et al.
In this retrospective, we review the two research topics that formed the basis of the outstanding career of Dr. Paul S. Frenette. In the first part, we focus on sickle cell disease (SCD). The defining feature of SCD is polymerization of the...
Nicole J C Narayan,David Requena,Gadi Lalazar et al.
Nicole J C Narayan et al.
Fibrolamellar carcinoma (FLC) is a rare, often lethal, liver cancer affecting adolescents and young adults, for which there are no approved therapeutics. The development of therapeutics is hampered by a lack of in vitro models. Organoids ha...