High-content phenotyping of Parkinson's disease patient stem cell-derived midbrain dopaminergic neurons using machine learning classification [0.03%]
基于患者诱导多能干细胞的帕金森病中脑多巴胺能神经元的高内涵表型分析及机器学习分类
Aurore Vuidel,Loïc Cousin,Beatrice Weykopf et al.
Aurore Vuidel et al.
Combining multiple Parkinson's disease (PD) relevant cellular phenotypes might increase the accuracy of midbrain dopaminergic neuron (mDAN) in vitro models. We differentiated patient-derived induced pluripotent stem cells (iPSCs) with a LRR...
Generation of a TPH2-EGFP reporter cell line for purification and monitoring of human serotonin neurons in vitro and in vivo [0.03%]
体外和体内纯化和监测人源血清素神经元的TPH2-EGFP报告细胞系的建立
Ting Xu,Jinjin Duan,Yingqi Li et al.
Ting Xu et al.
Generation of serotonin neurons (SNs) from human pluripotent stem cells (hPSCs) provides a promising platform to explore the mechanisms of serotonin-associated neuropsychiatric disorders. However, neural differentiation always yields hetero...
Single-cell spatiotemporal analysis reveals cell fates and functions of transplanted mesenchymal stromal cells during bone repair [0.03%]
单细胞时空分析揭示骨修复过程中移植间充质基质细胞的命运和功能
Chengyu Yang,Zeshun Li,Yang Liu et al.
Chengyu Yang et al.
Mesenchymal stromal cells (MSCs) transplantation could enhance bone repair. However, the cell fate of transplanted MSCs, in terms of their local distribution and spatial associations with other types of cells were poorly understood. Here, w...
Age-related pathological impairments in directly reprogrammed dopaminergic neurons derived from patients with idiopathic Parkinson's disease [0.03%]
特发性帕金森病患者重编程的多巴胺能神经元中的年龄相关病理损害
Janelle Drouin-Ouellet,Emilie M Legault,Fredrik Nilsson et al.
Janelle Drouin-Ouellet et al.
We have developed an efficient approach to generate functional induced dopaminergic (DA) neurons from adult human dermal fibroblasts. When performing DA neuronal conversion of patient fibroblasts with idiopathic Parkinson's disease (PD), we...
Single-cell mapping of regenerative and fibrotic healing responses after musculoskeletal injury [0.03%]
肌肉骨骼损伤后再生和纤维化修复反应的单细胞图谱绘制工作
Robert J Tower,Alec C Bancroft,Ashish R Chowdary et al.
Robert J Tower et al.
After injury, a cascade of events repairs the damaged tissue, including expansion and differentiation of the progenitor pool and redeposition of matrix. To guide future wound regeneration strategies, we compared single-cell sequencing of re...
PIM3-AMPK-HDAC4/5 axis restricts MuERVL-marked 2-cell-like state in embryonic stem cells [0.03%]
PIM3-AMPK-HDAC4/5轴限制胚胎干细胞中带有MuERVL标记的2细胞样状态
Xin Zhao,Jian Shen,Xuan Zhao et al.
Xin Zhao et al.
A minority of embryonic stem cells (ESCs) marked by endogenous retrovirus MuERVL are totipotent 2-cell-like cells. However, the majority of ESCs repress MuERVL. Currently, it is still unclear regarding the signaling pathway(s) repressing th...
Space microgravity improves proliferation of human iPSC-derived cardiomyocytes [0.03%]
微重力促进人类诱导多能干细胞来源心肌细胞的增殖
Antonio Rampoldi,Parvin Forghani,Dong Li et al.
Antonio Rampoldi et al.
In microgravity, cells undergo profound changes in their properties. However, how human cardiac progenitors respond to space microgravity is unknown. In this study, we evaluated the effect of space microgravity on differentiation of human i...
Investigation of PTC124-mediated translational readthrough in a retinal organoid model of AIPL1-associated Leber congenital amaurosis [0.03%]
PTC124介导的AIPL1相关Leber先天性黑蒙视网膜类器官模型读码终止的研究
Amy Leung,Almudena Sacristan-Reviriego,Pedro R L Perdigão et al.
Amy Leung et al.
Leber congenital amaurosis type 4 (LCA4), caused by AIPL1 mutations, is characterized by severe sight impairment in infancy and rapidly progressing degeneration of photoreceptor cells. We generated retinal organoids using induced pluripoten...
Frizzled-6 promotes hematopoietic stem/progenitor cell mobilization and survival during LPS-induced emergency myelopoiesis [0.03%]
弗林佐林-6促进脂多糖诱导的应急髓系增殖中造血干细胞/祖细胞动员和存活
Trieu Hai Nguyen,Belma Melda Abidin,Krista M Heinonen
Trieu Hai Nguyen
Emergency hematopoiesis involves the activation of bone marrow hematopoietic stem/progenitor cells (HSPCs) in response to systemic inflammation by a combination of cell-autonomous and stroma-dependent signals and leads to their release from...
In vitro modeling and rescue of ciliopathy associated with IQCB1/NPHP5 mutations using patient-derived cells [0.03%]
基于患者来源细胞的IQCB1/NPHP5突变相关纤毛病的体外建模和治疗研究
Kamil Kruczek,Zepeng Qu,Emily Welby et al.
Kamil Kruczek et al.
Mutations in the IQ calmodulin-binding motif containing B1 (IQCB1)/NPHP5 gene encoding the ciliary protein nephrocystin 5 cause early-onset blinding disease Leber congenital amaurosis (LCA), together with kidney dysfunction in Senior-Løken...