Generation of functionally competent hepatic stellate cells from human stem cells to model liver fibrosis in vitro [0.03%]
体外通过人类干细胞分化生成具有功能的肝星状细胞以模拟肝纤维化现象
Xinyuan Lai,Chuanyun Li,Chengang Xiang et al.
Xinyuan Lai et al.
The detailed understanding of fibrogenesis has been hampered by a lack of important functional quiescence characteristics and an in vitro model to recapitulate hepatic stellate cell (HSC) activation. In our study, we establish robust endode...
Dafni Chondronasiou,Jaime Martínez de Villarreal,Elena Melendez et al.
Dafni Chondronasiou et al.
Differentiated cells can be converted into pluripotent stem cells by expressing the transcription factors OCT4, SOX2, KLF4, and MYC (OSKM) in a process known as reprogramming. Here, using single-cell RNA sequencing of pancreas undergoing re...
Human primed and naïve PSCs are both able to differentiate into trophoblast stem cells [0.03%]
人源多能干细胞既能分化为滋养细胞干细胞
Sergey Viukov,Tom Shani,Jonathan Bayerl et al.
Sergey Viukov et al.
The recent derivation of human trophoblast stem cells (TSCs) from placental cytotrophoblasts and blastocysts opened opportunities for studying the development and function of the human placenta. Recent reports have suggested that human naï...
Entropy sorting of single-cell RNA sequencing data reveals the inner cell mass in the human pre-implantation embryo [0.03%]
熵排序单细胞RNA测序数据揭示人类植入前胚胎的内细胞团
Arthur Radley,Elena Corujo-Simon,Jennifer Nichols et al.
Arthur Radley et al.
A major challenge in single-cell gene expression analysis is to discern meaningful cellular heterogeneity from technical or biological noise. To address this challenge, we present entropy sorting (ES), a mathematical framework that distingu...
Molecular pathology of Usher 1B patient-derived retinal organoids at single cell resolution [0.03%]
Usher 1B患者视网膜类器官单细胞分辨率的分子病理学
Yeh Chwan Leong,Valentina Di Foggia,Hema Pramod et al.
Yeh Chwan Leong et al.
Usher syndrome-associated retinitis pigmentosa (RP) causes progressive retinal degeneration, which has no cure. The pathomechanism of Usher type 1B (USH1B)-RP caused by MYO7A mutation remains elusive because of the lack of faithful animal m...
Mesenchymal stem cell transplantation improves biomechanical properties of vaginal tissue following full-thickness incision in aged rats [0.03%]
骨髓间充质干细胞移植改善完全层切伤老年大鼠阴道组织生物力学性能
Ofra Ben Menachem-Zidon,Michal Gropp,Benjamin Reubinoff et al.
Ofra Ben Menachem-Zidon et al.
Pelvic organ prolapse (POP) is common among post-menopausal women and is associated with bladder, bowel, and sexual dysfunction. Surgical repair with the patients' native tissues is sub-optimal with high reoperation rates, potentially due t...
Human retinal organoids harboring IMPG2 mutations exhibit a photoreceptor outer segment phenotype that models advanced retinitis pigmentosa [0.03%]
携带IMPG2突变的人视网膜类器官表现出外段表型以模拟晚期色素性视网膜炎
Steven J Mayerl,Simona Bajgai,Allison L Ludwig et al.
Steven J Mayerl et al.
Interphotoreceptor matrix proteoglycan 2 (IMPG2) mutations cause a severe form of early-onset retinitis pigmentosa (RP) with macular involvement. IMPG2 is expressed by photoreceptors and incorporated into the matrix that surrounds the inner...
TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids [0.03%]
TGFβ信号调控人类干细胞多能性及其形成结构良好的大脑皮层类器官的能力
Momoko Watanabe,Jessie E Buth,Jillian R Haney et al.
Momoko Watanabe et al.
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid tec...
PI3Kβ-regulated β-catenin mediates EZH2 removal from promoters controlling primed human ESC stemness and primitive streak gene expression [0.03%]
PI3Kβ调节的β-连环蛋白介导EZH2从调控人胚干细胞多能性及原始条纹基因表达启动子上的移除
Sudhanshu Yadav,Antonio Garrido,M Carmen Hernández et al.
Sudhanshu Yadav et al.
The mechanism governing the transition of human embryonic stem cells (hESCs) toward differentiated cells is only partially understood. To explore this transition, the activity and expression of the ubiquitous phosphatidylinositol 3-kinase (...
Panning for gold: Purifying mesencephalic dopaminergic progenitors differentiated from human pluripotent stem cells [0.03%]
从人多能干细胞中分离提纯纹状体多巴胺能神经前体细胞
Qingyuan Tang,Jeffrey S Schweitzer,Bin Song
Qingyuan Tang
In a recently published study, Xu et al. used two surface markers, CLSTN2 and PTPRO, to generate highly purified donor dopaminergic neurons and achieved stable and predictable therapeutic outcomes by transplantation into the brain of PD ani...