What has single-cell transcriptomics taught us about long non-coding RNAs in the ventricular-subventricular zone? [0.03%]
单细胞转录组学向我们揭示了关于室下层中的长链非编码RNA的什么信息？
Jemima Becker,Bin Sun,Farah Alammari et al.
Jemima Becker et al.
Long non-coding RNA (lncRNA) function is mediated by the process of transcription or through transcript-dependent associations with proteins or nucleic acids to control gene regulatory networks. Many lncRNAs are transcribed in the ventricul...
Titin-truncating variants in hiPSC cardiomyocytes induce pathogenic proteinopathy and sarcomere defects with preserved core contractile machinery [0.03%]
Titin截短突变的hiPSC心肌细胞诱导致病性蛋白病变和保存核心收缩机制的肌节缺陷
Guanyi Huang,Anjali Bisaria,Devin L Wakefield et al.
Guanyi Huang et al.
Titin-truncating variants (TTNtv) are the single largest genetic cause of dilated cardiomyopathy (DCM). In this study we modeled disease phenotypes of A-band TTNtv-induced DCM in human induced pluripotent stem cell-derived cardiomyocytes (h...
Valentina Fossati,Valentina Greco,Paola Arlotta et al.
Valentina Fossati et al.
Integrated transcriptome-proteome analyses of human stem cells reveal source-dependent differences in their regenerative signature [0.03%]
人类干细胞的整合转录组和蛋白质组分析揭示了其再生信号的来源相关差异
Abantika Ganguly,Ganesh Swaminathan,Fernando Garcia-Marques et al.
Abantika Ganguly et al.
Mesenchymal stem cells (MSCs) are gaining increasing prominence as an effective regenerative cellular therapy. However, ensuring consistent and reliable effects across clinical populations has proved to be challenging. In part, this can be ...
Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation [0.03%]
人类内皮细胞分化中的动态染色质组织和调控相互作用
Kris G Alavattam,Katie A Mitzelfelt,Giancarlo Bonora et al.
Kris G Alavattam et al.
Vascular endothelial cells are a mesoderm-derived lineage with many essential functions, including angiogenesis and coagulation. The gene-regulatory mechanisms underpinning endothelial specialization are largely unknown, as are the roles of...
Stress-induced reversible cell-cycle arrest requires PRC2/PRC1-mediated control of mitophagy in Drosophila germline stem cells and human iPSCs [0.03%]
由压力诱导的可逆细胞周期阻滞需要果蝇生殖干细胞和人类诱导多能干细胞中PRC2/PRC1对线粒体自噬的调控作用
Tommy H Taslim,Abdiasis M Hussein,Riya Keshri et al.
Tommy H Taslim et al.
Following acute genotoxic stress, both normal and tumorous stem cells can undergo cell-cycle arrest to avoid apoptosis and later re-enter the cell cycle to regenerate daughter cells. However, the mechanism of protective, reversible prolifer...
Epigenetic biomarkers to track differentiation of pluripotent stem cells [0.03%]
用于追踪多能干细胞分化的表观遗传生物标志物
Marco Schmidt,Kira Zeevaert,Mohamed H Elsafi Mabrouk et al.
Marco Schmidt et al.
Quality control of induced pluripotent stem cells remains a challenge. For validation of the pluripotent state, it is crucial to determine trilineage differentiation potential toward endoderm, mesoderm, and ectoderm. Here, we report GermLay...
Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations [0.03%]
使用3D打印指南从单个老鼠中同时隔离肺近端和远端祖细胞可减少远端肺上皮细胞分离中的近端细胞污染
Hani N Alsafadi,John Stegmayr,Victoria Ptasinski et al.
Hani N Alsafadi et al.
The respiratory epithelium consists of multiple, functionally distinct cell types and is maintained by regionally specific progenitor populations that repair the epithelium following injury. Several in vitro methods exist for studying lung ...
Modeling sex differences in humans using isogenic induced pluripotent stem cells [0.03%]
利用同基因诱导的多能干细胞对人类的性别差异进行建模
Ithai Waldhorn,Tikva Turetsky,Debora Steiner et al.
Ithai Waldhorn et al.
Biological sex is a fundamental trait influencing development, reproduction, pathogenesis, and medical treatment outcomes. Modeling sex differences is challenging because of the masking effect of genetic variability and the hurdle of differ...
A single cell-based computational platform to identify chemical compounds targeting desired sets of transcription factors for cellular conversion [0.03%]
一个基于单细胞的计算平台，用于识别针对所需转录因子集进行细胞转化的化学化合物
Menglin Zheng,Bingqing Xie,Satoshi Okawa et al.
Menglin Zheng et al.
Cellular conversion can be induced by perturbing a handful of key transcription factors (TFs). Replacement of direct manipulation of key TFs with chemical compounds offers a less laborious and safer strategy to drive cellular conversion for...