Kenji Kamimoto,Mohd Tayyab Adil,Kunal Jindal et al.
Kenji Kamimoto et al.
In direct lineage conversion, transcription factor (TF) overexpression reconfigures gene regulatory networks (GRNs) to reprogram cell identity. We previously developed CellOracle, a computational method to infer GRNs from single-cell transc...
Retention of ERK in the cytoplasm mediates the pluripotency of embryonic stem cells [0.03%]
ERK保留在细胞质中介导了胚胎干细胞的多能性
Avital Hacohen Lev-Ran,Rony Seger
Avital Hacohen Lev-Ran
The dynamic subcellular localization of ERK1/2 plays an important role in regulating cell fate. Differentiation of mouse embryonic stem cells (mESCs) involves inductive stimulation of ERK1/2, and therefore, inhibitors of the ERK cascade are...
Small-molecule screen reveals pathways that regulate C4 secretion in stem cell-derived astrocytes [0.03%]
小分子筛选发现调控干细胞衍生星形胶质细胞C4分泌的信号通路
Francesca Rapino,Ted Natoli,Francesco Limone et al.
Francesca Rapino et al.
In the brain, the complement system plays a crucial role in the immune response and in synaptic elimination during normal development and disease. Here, we sought to identify pathways that modulate the production of complement component 4 (...
Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures [0.03%]
揭示在原始多能干细胞培养物中捕获人类胚胎早期发育阶段的细胞群落
Marta Moya-Jódar,Asier Ullate-Agote,Paula Barlabé et al.
Marta Moya-Jódar et al.
Naive human pluripotent stem cells (hPSCs) are defined as the in vitro counterpart of the human preimplantation embryo's epiblast and are used as a model system to study developmental processes. In this study, we report the discovery and ch...
Roger A Barker,Melissa Carpenter,Catriona H M Jamieson et al.
Roger A Barker et al.
Developing cellular therapies is not straightforward. This Perspective summarizes the experience of a group of academic stem cell investigators working in different clinical areas and aims to share insight into what we wished we knew before...
The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation [0.03%]
在肝源性成熟过程中观察到的蛋白质合成的全局下调与TOP mRNA翻译减少有关
Marino Caruso,Sébastien Meurant,Damien Detraux et al.
Marino Caruso et al.
Translational regulation is of paramount importance for proteome remodeling during stem cell differentiation at both the global and the transcript-specific levels. In this study, we characterized translational remodeling during hepatogenic ...
CFIm-mediated alternative polyadenylation safeguards the development of mammalian pre-implantation embryos [0.03%]
CFI_m介导的可变多聚腺苷酸化保障了哺乳动物植入前胚胎的发育
Na Li,Ying Cai,Min Zou et al.
Na Li et al.
Alternative polyadenylation (APA) gives rise to transcripts with distinct 3' untranslated regions (3' UTRs), thereby affecting the fate of mRNAs. APA is strongly associated with cell proliferation and differentiation status, and thus likely...
Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures [0.03%]
长期钙成像显示hiPSC培养物的功能发展与人相似，但与大鼠原代培养物不同
Estefanía Estévez-Priego,Martina Moreno-Fina,Emanuela Monni et al.
Estefanía Estévez-Priego et al.
Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived ...
Production of large, defined genome modifications in rats by targeting rat embryonic stem cells [0.03%]
通过定位大鼠胚胎干细胞产生大型的，定义明确的大鼠基因组修改
Jeffrey Lee,Jingjing Wang,Roxanne Ally et al.
Jeffrey Lee et al.
Rats were more frequently used than mice to model human disease before mouse embryonic stem cells (mESCs) revolutionized genetic engineering in mice. Rat ESCs (rESCs) were first reported over 10 years ago, yet they are not as frequently use...
Dysregulated Polycomb Repressive Complex 2 contributes to chronic obstructive pulmonary disease by rewiring stem cell fate [0.03%]
失调的多梳抑制复合物2通过改变干细胞命运导致慢性阻塞性肺疾病
Aria L Byrd,Xufeng Qu,Alexsandr Lukyanchuk et al.
Aria L Byrd et al.
Aberrant lung cell differentiation is a hallmark of many lung diseases including chronic obstructive pulmonary disease (COPD). The EZH2-containing Polycomb Repressive Complex 2 (PRC2) regulates embryonic lung stem cell fate, but its role in...