Complex haploinsufficiency in pluripotent cells yields somatic cells with DNA methylation abnormalities and pluripotency induction defects [0.03%]
胚胎干细胞中单倍体不足通过复杂调控产生畸变多能性细胞
Rachel Lasry,Noam Maoz,Albert W Cheng et al.
Rachel Lasry et al.
A complete knockout of a single key pluripotency gene may drastically affect embryonic stem cell function and epigenetic reprogramming. In contrast, elimination of only one allele of a single pluripotency gene is mostly considered harmless ...
Synthetic augmentation of bilirubin metabolism in human pluripotent stem cell-derived liver organoids [0.03%]
人多能干细胞系肝类器官胆红素代谢的合成增强
Hasan Al Reza,Zishaan Farooqui,Abid Al Reza et al.
Hasan Al Reza et al.
UGT1A1 (UDP glucuronosyltransferase family 1 member A1) is the primary enzyme required for bilirubin conjugation, which is essential for preventing hyperbilirubinemia. Animal models lack key human organic anion transporting polypeptides wit...
Ariel Simpson,Alex W Hewitt,Kirsten A Fairfax
Ariel Simpson
Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold promise for transplantation medicine. Diverse human leukocyte antigen (HLA) profiles necessitate autologous cells or multiple cell lines for therapeutics, inc...
PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes [0.03%]
PTBP2抑制通过促进神经元基因的可变剪接促进成纤维细胞向神经元的重编程
Binglin Zhu,Emily Fisher,Li Li et al.
Binglin Zhu et al.
The direct conversion of human skin fibroblasts to neurons has a low efficiency and unclear mechanism. Here, we show that the knockdown of PTBP2 significantly enhanced the transdifferentiation induced by ASCL1, MIR9/9∗-124, and p53 shRNA (...
Single-cell RNA sequencing of human epidermis identifies Lunatic fringe as a novel regulator of the stem cell compartment [0.03%]
单细胞RNA测序揭示Lunatic fringe是调节人体表皮干细胞的新因子
Victor Augusti Negri,Blaise Louis,Sebastiaan Zijl et al.
Victor Augusti Negri et al.
Single-cell RNA sequencing (scRNA-seq) of human skin provides a tool for validating observations from in vitro experimental models. By analyzing a published dataset of healthy adult epidermis, we confirm that the basal epidermal layer is he...
Polycomb repressive complex 2 regulates basal cell fate during adult olfactory neurogenesis [0.03%]
多梳抑制复合体2在成人嗅觉神经发生过程中调节基底细胞命运
Tiffany Ko,Rhea Choi,Khalil Issa et al.
Tiffany Ko et al.
Adult neurogenesis occurs in the mammalian olfactory epithelium to maintain populations of neurons that are vulnerable to injury yet essential for olfaction. Multipotent olfactory basal stem cells are activated by damage, although mechanism...
Ulgu Arslan,Marcella Brescia,Viviana Meraviglia et al.
Ulgu Arslan et al.
Published Erratum
Stem cell reports. 2023 Oct 10;18(10):2003. DOI:10.1016/j.stemcr.2023.08.012 2023
Rare intercellular material transfer as a confound to interpreting inner retinal neuronal transplantation following internal limiting membrane disruption [0.03%]
视网膜内界膜破裂后解读视网膜神经元移植的罕见细胞间物质转移干扰因素
Kevin Y Zhang,Arumugam Nagalingam,Stella Mary et al.
Kevin Y Zhang et al.
Intercellular cytoplasmic material transfer (MT) occurs between transplanted and developing photoreceptors and ambiguates cell origin identification in developmental, transdifferentiation, and transplantation experiments. Whether MT is a ph...
ERRγ agonist under mechanical stretching manifests hypertrophic cardiomyopathy phenotypes of engineered cardiac tissue through maturation [0.03%]
应机械牵张的 ERRγ 激动剂通过成熟诱导工程化心肌组织产生心脏肥大表型
Yuya Fujiwara,Kenji Miki,Kohei Deguchi et al.
Yuya Fujiwara et al.
Engineered cardiac tissue (ECT) using human induced pluripotent stem cell-derived cardiomyocytes is a promising tool for modeling heart disease. However, tissue immaturity makes robust disease modeling difficult. Here, we established a meth...
The N-terminus of Stag1 is required to repress the 2C program by maintaining rRNA expression and nucleolar integrity [0.03%]
Stag1的N端通过维持rRNA表达和核仁完整性抑制2C程序所必需
Dubravka Pezic,Samuel Weeks,Wazeer Varsally et al.
Dubravka Pezic et al.
Our understanding of how STAG proteins contribute to cell identity and disease have largely been studied from the perspective of chromosome topology and protein-coding gene expression. Here, we show that STAG1 is the dominant paralog in mou...