Characterization of perivascular alveolar epithelial stem cells and their niche in lung homeostasis and cancer [0.03%]
肺稳态和癌症中的血管周围肺泡上皮干细胞及其生态位的特征分析
Qian Chen,Hiroyuki Hirai,Manwai Chan et al.
Qian Chen et al.
Lung alveolar structure and function are maintained by subsets of alveolar type II stem cells (AT2s), but there is a need for characterization of these subsets and their associated niches. Here, we report a CD44high subpopulation of AT2s ch...
Rigor and reproducibility in human brain organoid research: Where we are and where we need to go [0.03%]
人体脑器官研究的严谨性和可重复性：现状与未来方向
Soraya O Sandoval,Gerarda Cappuccio,Karina Kruth et al.
Soraya O Sandoval et al.
Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation i...
Functional mouse hepatocytes derived from interspecies chimeric livers effectively mitigate chronic liver fibrosis [0.03%]
取自异种嵌合肝的具功能小鼠肝细胞可有效缓解慢性肝纤维化
Cheng Huang,Haiping Jiang,Jingxi Dong et al.
Cheng Huang et al.
Liver disease is a major global health challenge. There is a shortage of liver donors worldwide, and hepatocyte transplantation (HT) may be an effective treatment to overcome this problem. However, the present approaches for generation of h...
Victor L Perez,Hazem M Mousa,Kiyoharu J Miyagishima et al.
Victor L Perez et al.
Several gaps and barriers remain for transplanting stem cells into the eye to treat ocular disease, especially diseases of the retina. While the eye has historically been considered immune privileged, recent thinking has identified the immu...
Reduction of H3K9 methylation by G9a inhibitors improves the development of mouse SCNT embryos [0.03%]
G9a抑制剂诱导的H3K9去甲基化能改善小鼠体细胞克隆胚胎的发育能力
Shogo Matoba,Daiki Shikata,Fumiyuki Shirai et al.
Shogo Matoba et al.
Removal of somatic histone H3 lysine 9 trimethylation (H3K9me3) from the embryonic genome can improve the efficiency of mammalian cloning using somatic cell nuclear transfer (SCNT). However, this strategy involves the injection of histone d...
H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency [0.03%]
H1FOO-DD促进向原始多能性重编程的效率和均匀性
Akira Kunitomi,Ryoko Hirohata,Mitsujiro Osawa et al.
Akira Kunitomi et al.
Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to a destabilizing domain (H1FOO-DD), together with OCT4...
Identification of unapproved orthopedic regenerative medicine: Usefulness of the Act on Safety of Regenerative Medicine [0.03%]
未经审批的骨科再生医药制品鉴定：《再生医药安全法》的有效性分析
Yuichiro Ukon,Satoshi Hosoya,Kazuki Morita et al.
Yuichiro Ukon et al.
In Japan, the Act on Safety of Regenerative Medicine regulates unapproved regenerative medicine. Other nations market regenerative medicine products, bypassing regulatory approval. To identify unapproved orthopedic regenerative medicine, we...
The Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development [0.03%]
Wnt依赖的主调节因子NKX1-2调控小鼠着床前胚胎发育
Shoma Nakagawa,Davide Carnevali,Xiangtian Tan et al.
Shoma Nakagawa et al.
Embryo size, specification, and homeostasis are regulated by a complex gene regulatory and signaling network. Here we used gene expression signatures of Wnt-activated mouse embryonic stem cell (mESC) clones to reverse engineer an mESC regul...
Unraveling the impact of ZZZ3 on the mTOR/ribosome pathway in human embryonic stem cells homeostasis [0.03%]
揭示锌指抗凋亡蛋白ZZZ3对人胚胎干细胞稳态中mTOR/核糖体途径影响的作用机制
Michela Lo Conte,Valeria Lucchino,Stefania Scalise et al.
Michela Lo Conte et al.
Embryonic stem cells (ESCs) are defined as stem cells with self-renewing and differentiation capabilities. These unique properties are tightly regulated and controlled by complex genetic and molecular mechanisms, whose understanding is esse...
Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility [0.03%]
针对类器官芯片建立质量管理体系及监控其可重复性的量身定制标准的建议
David Pamies,Jason Ekert,Marie-Gabrielle Zurich et al.
David Pamies et al.
Cell culture technology has evolved, moving from single-cell and monolayer methods to 3D models like reaggregates, spheroids, and organoids, improved with bioengineering like microfabrication and bioprinting. These advancements, termed micr...