Three-dimensional co-culturing reveals human stem cell-derived somatostatin interneurons with subclass expression [0.03%]
三维共培养揭示了表达亚类的人类干细胞来源的生长抑素中间神经元
Andreas Bruzelius,Christina-Anastasia Stamouli,Anna-Lena Hölldobler et al.
Andreas Bruzelius et al.
Cortical interneuron deficiencies, particularly involving the somatostatin (SST) subtypes, contribute to neurological and neuropsychiatric disorders. These interneurons are difficult to derive in vitro from human embryonic stem cells (hESCs...
Single-cell glycome and transcriptome profiling uncovers the glycan signature of each cell subpopulation of human iPSC-derived neurons [0.03%]
单细胞糖组和转录组分析揭示人多能干细胞来源神经元亚群的糖链标志物
Haruki Odaka,Hiroaki Tateno
Haruki Odaka
Human induced pluripotent stem cell (iPSC)-derived neurons are often heterogeneous, posing challenges for disease modeling and cell therapy. We previously developed single-cell glycan and RNA sequencing (scGR-seq) to analyze the glycome and...
TAOK2 drives opposing cilia length deficits in 16p11.2 deletion and duplication carriers [0.03%]
TAOK2驱动16p11.2缺失和重复携带者的相反的纤毛长度缺陷
Sujin Byeon,Amy Ferreccio,Moira Cornell et al.
Sujin Byeon et al.
Deletion and duplication of the 16p11.2 genomic locus are associated with opposing changes in brain size. To determine cellular mechanisms that underlie these opposing phenotypes, we performed quantitative phosphoproteomic analyses of induc...
Pen-2 regulates glial homeostasis by coordinating self-renewal and transdifferentiation programs in oligodendrocyte precursor cells [0.03%]
少突胶质前体细胞中的笔-2通过协调自我更新和转分化程序调节神经胶质稳态
Huiru Bi,Jinxing Hou,Wenkai Shao et al.
Huiru Bi et al.
Presenilin enhancer 2 (Pen-2) is implicated in neurological diseases characterized by glial dysregulation. To investigate whether oligodendrocytic γ-secretase activity is important for the maintenance of glial populations, we analyzed two ...
Single-cell transcriptome and surfaceome profiling of the adult human retinal pigment epithelium [0.03%]
成人视网膜色素上皮的单细胞转录组和表面ome谱型分析
Farhad Farjood,Swapna Nandakumar,Taylor Bertucci et al.
Farhad Farjood et al.
The retinal pigment epithelium (RPE) is a pigmented monolayer of cells beneath the neural retina that supports photoreceptor cell function essential for vision. Our study explores the diversity of adult human RPE subpopulations and associat...
Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans [0.03%]
抑制多能干细胞中的N-十四酰基转移酶可使小鼠保持初始态并使人诱导出滋养层和原始内胚层标记物
Junko Yoshida,Hitomi Watanabe,Kaori Yamauchi et al.
Junko Yoshida et al.
Naive and primed states represent distinct phases of pluripotency during early embryonic development, both of which can be captured and interconverted in vitro. To understand pluripotency regulation, we performed a recessive genetic screen ...
ApoE4 disrupts intracellular trafficking and iron homeostasis in a reproducible iPSC-based model of human brain endothelial cells [0.03%]
APOE4会破坏人类脑内皮细胞诱导多能干细胞模型中的胞内转运和铁稳态
Luisa Bell,Shane Clerkin,Sila Rizalar et al.
Luisa Bell et al.
Transferrin receptor in brain endothelial cells can deliver therapeutic antibodies to the brain via transcytosis across the blood-brain barrier (BBB). Whether receptor transport remains intact in Alzheimer disease is still a major open ques...
Computationally resolved neuroprogenitor cell biomarkers associate with human disorders [0.03%]
计算解析的神经前体细胞生物标志物与人类疾病相关联
Gerarda Cappuccio,William T Choi,Fatih Semerci et al.
Gerarda Cappuccio et al.
Adult hippocampal neurogenesis, the process of generating new neurons, relies on a rare population of neural stem and progenitor cells (NPCs) within the dentate gyrus complex microenvironment. Discovering the specific genes that define thes...
XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector [0.03%]
利用模块化抗沉默载体对人多能干细胞进行快速遗传工程改造以实现持久过表达的XPRESSO方法
Yehuda Wexler,Harel Grinstein,Irit Huber et al.
Yehuda Wexler et al.
Ectopic expression of proteins in human pluripotent stem cells (hPSCs) is highly desirable as a research tool and important for clinical translation. However, genetically engineering hPSCs for long-term overexpression of proteins remains in...
Toti: An integrated multi-omics database to decipher the epigenetic regulation of gene expression in totipotent stem cells [0.03%]
Toti：一个集成的多组学数据库 用于解析全能干细胞中基因表达的表观遗传调控机制
Yi Chai,Ruiying Zhang,Shunze Jia et al.
Yi Chai et al.
Totipotent stem cells (TSCs), the origin of mammalian life and the foundation of early embryogenesis, possess the highest differentiation capacity and extensive developmental potential. However, none of the existing embryonic databases have...