Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels [0.03%]
胎生矩阵沉积支持合成水凝胶中聚集物肺泡类器官的形成
Madeline K Eiken,Charlie J Childs,Lindy K Brastrom et al.
Madeline K Eiken et al.
Human induced pluripotent stem cell (iPSC)-derived alveolar organoids have emerged as a system to model the alveolar epithelium in homeostasis and disease. However, alveolar organoids are typically grown in Matrigel, a mouse sarcoma-derived...
Kouta Niizuma,Satoru Morikawa,Eric Gars et al.
Kouta Niizuma et al.
Hematopoietic stem cells (HSCs) are crucial for maintaining hematopoietic homeostasis and are localized within distinct bone marrow (BM) niches. While BM niches are often considered similar across different skeletal sites, we discovered tha...
Message from the editor-in-chief [0.03%]
主编来信
Martin F Pera
Martin F Pera
Glycogen synthase kinase-3 inhibition and insulin enhance proliferation and inhibit maturation of human iPSC-derived cardiomyocytes via TCF and FOXO signaling [0.03%]
糖原合成酶激酶-3抑制和胰岛素通过TCF和FOXO信号增强人类iPSC来源的心肌细胞增殖并抑制其成熟
Qianliang Yuan,Devin Verbueken,Rafeeh Dinani et al.
Qianliang Yuan et al.
Embryonic signaling pathways exert stage-specific effects during cardiac development, yet the precise signals for proliferation or maturation remain elusive. To uncover the cues for proliferation, we performed a combinatory cell-cycle scree...
Optimized prime editing of the Alzheimer's disease-associated APOE4 mutation [0.03%]
APOE4阿尔茨海默病相关突变的优化质粒及递送系统编辑疗法
Antje K Rottner,Anders Lundin,Songyuan Li et al.
Antje K Rottner et al.
Gene editing strategies to safely and robustly modify the Alzheimer's disease-associated APOE4 isoform are still lacking. Prime editing (PE) enables the precise introduction of genetic variants with minimal unintended editing and without do...
A versatile in vivo platform for reversible control of transgene expression in adult tissues [0.03%]
一种用于在成人组织中逆转录基因表达的通用体内平台
Jumpei Taguchi,Yosuke Yamada,Sho Ohta et al.
Jumpei Taguchi et al.
Temporal control of transgenes has advanced biomedical interventions, including in vivo reprogramming, often utilizing the doxycycline (Dox)-mediated Tet-ON system. Here, we developed the Dox-mediated Tet-ON or complementary Tet-OFF counter...
Deep-supercooling preservation of stem cell spheroids for chondral defect repairment [0.03%]
深度超冷保存干细胞球体以修复软骨缺损
Jinbin Qiu,Bangrui Yu,Cheng Ren et al.
Jinbin Qiu et al.
Versatile mesenchymal stem cells (MSCs) play an important role in tissue engineering and regenerative medicine. MSCs in 3D spheroid have shown higher secretion and differentiation functions than suspended counterparts, and, thus, in vitro c...
Tsunakuni Ikka,Taichi Hatta,Misao Fujita
Tsunakuni Ikka
The key amendment to the Act on the Safety of Regenerative Medicine in June 2024 is regarding on-site inspections and the criteria for disqualifying the Certified Special Committees for Regenerative Medicine and Certified Committees for Reg...
Spatial transcriptomics reveals molecular cues underlying the site specificity of the adult mouse oral mucosa and its stem cell niches [0.03%]
空间转录组学揭示了成年小鼠口腔黏膜及其干细胞生态位定位特异性的分子特征
Anna C Seubert,Marion Krafft,Sarah Bopp et al.
Anna C Seubert et al.
The oral cavity is a multifunctional organ composed of structurally heterogeneous mucosal tissues that remain poorly characterized. Oral mucosal tissues are highly stratified and segmented along an epithelial-lamina propria axis. Here, we p...
Bone morphogenetic protein 4 induces hematopoietic stem cell development from murine hemogenic endothelial cells in culture [0.03%]
骨形态发生蛋白4能诱导小鼠生血内皮细胞在体外向造血干细胞分化
Mariko Tsuruda,Saori Morino-Koga,Xueyu Zhao et al.
Mariko Tsuruda et al.
Hematopoietic stem cells (HSCs) develop from hemogenic endothelial cells (HECs) during mouse embryogenesis. Understanding the signaling molecules required for HSC development is crucial for the in vitro derivation of HSCs. We previously ind...