Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids [0.03%]
用于人类内耳类器官的化学定义和动态点击水凝胶支持听力细胞分化
Matthew R Arkenberg,Mahboubeh Jafarkhani,Chien-Chi Lin et al.
Matthew R Arkenberg et al.
The mechanical properties in the inner ear microenvironment play a key role in its patterning during embryonic development. To recapitulate inner ear development in vitro, three-dimensional tissue engineering strategies including the applic...
Non-canonical roles of CFH in retinal pigment epithelial cells revealed by dysfunctional rare CFH variants [0.03%]
罕见的CFH变异引起的视网膜色素上皮细胞中CFH非经典功能的研究
Sofie C A Brink,Louet Koolen,Caroline C W Klaver et al.
Sofie C A Brink et al.
Complement factor H (CFH) common genetic variants have been associated with age-related macular degeneration (AMD). While most previous in vitro RPE studies focused on the common p.His402Tyr CFH variant, we characterized rare CFH variants t...
Astrocyte-derived PTN alleviates deficits in hippocampal neurogenesis and cognition in models of multiple sclerosis [0.03%]
星形胶质细胞衍生的PTN可缓解多发性硬化模型中海马神经发生和认知障碍缺陷
Yanna Song,Haoyang Li,Yuhan Li et al.
Yanna Song et al.
Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease that results in motor, sensory, cognitive, and affective deficits. Hippocampal demyelination, a common occurrence in MS, is linked to impaired cognitive function an...
Heparan sulfate regulates the fate decisions of human pluripotent stem cells [0.03%]
硫酸肝素调节人多能干细胞的命运决定
Deepsing Syangtan,Deena Al Mahbuba,Sayaka Masuko et al.
Deepsing Syangtan et al.
Heparan sulfate (HS) is an anionic polysaccharide generated by all animal cells, but our understanding of its roles in human pluripotent stem cell (hPSC) self-renewal and differentiation is limited. We derived HS-deficient hPSCs by disrupti...
Chemically defined and growth factor-free system for highly efficient endoderm induction of human pluripotent stem cells [0.03%]
一种用于诱导人多能干细胞向内胚层分化的无饲养细胞、无需生长因子的化学定义培养体系
Zhiju Zhao,Fanzhu Zeng,Yage Nie et al.
Zhiju Zhao et al.
Definitive endoderm (DE) derived from human pluripotent stem cells (hPSCs) holds great promise for cell-based therapies and drug discovery. However, current DE differentiation methods required undefined components and/or expensive recombina...
Exploiting O-GlcNAc dyshomeostasis to screen O-GlcNAc transferase intellectual disability variants [0.03%]
利用O-GlcNAc稳态失调筛选O-GlcNAc转移酶智力障碍变异型
Huijie Yuan,Conor W Mitchell,Andrew T Ferenbach et al.
Huijie Yuan et al.
O-GlcNAcylation is an essential protein modification catalyzed by O-GlcNAc transferase (OGT). Missense variants in OGT are linked to a novel intellectual disability syndrome known as OGT congenital disorder of glycosylation (OGT-CDG). The m...
Swift induction of human spinal lower motor neurons and robust ALS cell screening via single-cell imaging [0.03%]
快速诱导人类脊髓运动神经元及有效的ALS细胞筛选单细胞图谱技术
Selena Setsu,Satoru Morimoto,Shiho Nakamura et al.
Selena Setsu et al.
This study introduces a novel method for rapidly and efficiently inducing human spinal lower motor neurons (LMNs) from induced pluripotent stem cells (iPSCs) to eventually elucidate the pathomechanisms of amyotrophic lateral sclerosis (ALS)...
Samuel P Moss,Ezgi Bakirci,Adam W Feinberg
Samuel P Moss
Organoids form through the sel f-organizing capabilities of stem cells to produce a variety of differentiated cell and tissue types. Most organoid models, however, are limited in terms of the structure and function of the tissues that form,...
PKM2 is a key factor to regulate neurogenesis and cognition by controlling lactate homeostasis [0.03%]
PKM2通过调控乳酸稳态在神经发生和认知调节中起关键作用
Pengyan He,Bingjun Zhang,Wei Jiang et al.
Pengyan He et al.
Adult hippocampal neurogenesis (AHN), the process of generating new neurons from adult neural stem/progenitor cells (NSPCs), is crucial for cognitive functions and is influenced by numerous factors, including metabolic processes. Pyruvate k...
Low fucosylation defines the glycocalyx of progenitor cells and melanocytes in the human limbal stem cell niche [0.03%]
低岩藻糖基化定义了人眼睑缘干细胞龛中前体细胞和黑色素细胞糖萼的特征
Ashley M Woodward,Damien Guindolet,Rafael Martinez-Carrasco et al.
Ashley M Woodward et al.
It is widely recognized that the glycocalyx has significant implications in regulating the self-renewal and differentiation of adult stem cells; however, its composition remains poorly understood. Here, we show that the fucose-binding Aleur...