Autism- and intellectual disability-associated MYT1L mutation alters human cortical interneuron differentiation, maturation, and physiology [0.03%]
与自闭症和智力障碍相关的MYT1L突变改变了人类大脑皮层中间神经元的分化，成熟及生理特性
Ramachandran Prakasam,Julianna Determan,Gareth Chapman et al.
Ramachandran Prakasam et al.
Myelin transcription factor 1 like (MYT1L) is a neuronal transcription factor highly expressed in the developing and adult brain, and, while pathogenic MYT1L mutations cause neurodevelopmental disorders, these have not been characterized in...
A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State [0.03%]
一个完全定义的无需饲养层系统用于建立和保持人胚胎干性的原始状态
Iwona Szczerbinska,Kevin Andrew Uy Gonzales,Engin Cukuroglu et al.
Iwona Szczerbinska et al.
Published Erratum
Stem cell reports. 2025 Feb 27:102446. DOI:10.1016/j.stemcr.2025.102446 2025
An efficient, non-viral arrayed CRISPR screening platform for iPSC-derived myeloid and microglia models [0.03%]
用于iPSC来源的髓系和小胶质细胞模型的有效非病毒CRISPR筛选平台
Sonja Meier,Anne Sofie Gry Larsen,Florian Wanke et al.
Sonja Meier et al.
Here, we developed a CRISPR-Cas9 arrayed screen to investigate lipid handling pathways in human induced pluripotent stem cell (iPSC)-derived microglia. We established a robust method for the nucleofection of CRISPR-Cas9 ribonucleoprotein co...
Clonal lineage tracing and transcriptomics of cortical progenitor populations reveal maintenance of differentiation potential [0.03%]
皮层祖细胞群体的克隆系追踪和转录组学揭示了分化潜能的维持
Danyon Harkins,Shawar Ali,Teodora Tockovska et al.
Danyon Harkins et al.
Postnatal neocortical development is a complex period wherein radial glial progenitors (RGPs) complete excitatory neurogenesis and transition to the production of glia. Here, we take advantage of a multi-layered lineage tracing tool pbacBar...
Tracking and mitigating imprint erasure during induction of naive human pluripotency at single-cell resolution [0.03%]
在诱导人细胞达到原始态多能性的单细胞分辨率下的追踪和缓解印记擦除过程
Laura A Fischer,Brittany Meyer,Monica Reyes et al.
Laura A Fischer et al.
Naive human pluripotent stem cells (hPSCs) model the pre-implantation epiblast. However, parent-specific epigenetic marks (imprints) are eroded in naive hPSCs, which represents an important deviation from the epiblast in vivo. To track the ...
An acidic residue within the OCT4 dimerization interface of SOX17 is necessary and sufficient to overcome its pluripotency-inducing activity [0.03%]
SOX17中OCT4二聚化界面内的一个酸性残基对于其克服诱导多能性活性是必要和充分的
Sik Yin Ho,Haoqing Hu,Derek Hoi Hang Ho et al.
Sik Yin Ho et al.
SOX17 directs the differentiation toward endoderm and acts as a human germline specifier. We previously found that the replacement of glutamate at position 57 of the high-mobility group (HMG) box with the basic lysine residue in SOX2 alters...
Comment on "MSCohi-O lenses for long-term retention of mesenchymal stem cells on ocular surface as a therapeutic approach for chronic ocular graft-versus-host disease" [0.03%]
关于“间充质干细胞角膜接触镜长期贴附于眼部表面治疗慢性移植物抗宿主病的疗法”一文的评论
Xinxin Yu,Shuai Huang,Yizhuo Zhao et al.
Xinxin Yu et al.
20 years of stemness: From stem cells to hypertranscription and back [0.03%]
干细胞性二十年：从干细胞到超转录再回到干细胞性
Yun-Kyo Kim,Miguel Ramalho-Santos
Yun-Kyo Kim
Transcriptional profiling of stem cells came of age at the beginning of the century with the use of microarrays to analyze cell populations in bulk. Since then, stem cell transcriptomics has become increasingly sophisticated, notably with t...
Response to Yu et al [0.03%]
对余等人的回应
Yuanyue Liu,Siqi Song,Youyu Liu et al.
Yuanyue Liu et al.
ERK phosphorylates ESRRB to regulate the self-renewal and differentiation of mouse embryonic stem cells [0.03%]
ERK通过磷酸化ESRRB来调控小鼠胚胎干细胞的自我更新和分化过程
Xiaowei Duan,Qingye Zhang,Lulu Gao et al.
Xiaowei Duan et al.
MEK (mitogen-activated protein kinase) inhibitor is widely used for culturing pluripotent stem cells, while prolonged MEK inhibition compromises the developmental potential of mouse embryonic stem cells (ESCs), implying a dual role of MEK/E...