Millie Xin Barbernitz,Daniel M Raben
Millie Xin Barbernitz
Diacylglycerol kinases (DGKs) are a family of enzymes that catalyze the ATP-dependent conversion of diacylglycerol (DAG) to phosphatidic acid (PtdOH). A common approach to examine the activity of these enzymes relys on a radiometric assay (...
Structural basis for the conserved roles of PI4KA and its regulatory partners and their misregulation in disease [0.03%]
PI4KA及其调控伙伴在保守功能及其疾病中的失调的结构基础
Sushant Suresh,John E Burke
Sushant Suresh
The type III Phosphatidylinositol 4-kinase alpha (PI4KA) is an essential lipid kinase that is a master regulator of phosphoinositide signalling at the plasma membrane (PM). It produces the predominant pool of phosphatidylinositol 4-phosphat...
Miguel Martin-Salgado,Ane Ochoa-Echeverría,Isabel Mérida
Miguel Martin-Salgado
Cancer still represents the second leading cause of death right after cardiovascular diseases. According to the World Health Organization (WHO), cancer provoked around 10 million deaths in 2020, with lung and colon tumors accounting for the...
Ablation of diacylglycerol kinase ε promotes whitening of brown adipose tissue under high fat diet feeding [0.03%]
DGKE敲除通过促进高脂饮食诱导的棕色脂肪白色化改善代谢健康
Tomoyuki Nakano,Ayako Suzuki,Kaoru Goto
Tomoyuki Nakano
Adipose tissue (AT) comprises distinct fat depots such as white AT and brown AT. White and brown adipocytes exhibit different morphological and physiological properties. White adipocytes containing large single lipid droplet (LD) provide en...
Seon Hyang Park,Ji Hyeon Kang,Yoe-Sik Bae
Seon Hyang Park
Phospholipase D (PLD) is an enzyme that catalyzes the hydrolysis of phosphatidylcholine into phosphatidic acid and free choline. In mammals, PLD exists in two well-characterized isoforms, PLD1 and PLD2, and it plays pivotal roles as signali...
Proteomic and yeast 2-hybrid screens to identify PTEN binding partners [0.03%]
PTEN结合蛋白的蛋白质组及酵母双杂交筛选
Priyanka Tibarewal,Laura Spinelli,Helene Maccario et al.
Priyanka Tibarewal et al.
PTEN is a phosphoinositide lipid phosphatase and an important tumour suppressor protein. PTEN function is reduced or lost in around a third of all human cancers through diverse mechanisms, from gene deletion to changes in the function of pr...
Recent advances in the application of induced pluripotent stem cell technology to the study of myeloid malignancies [0.03%]
诱导多能干细胞技术在髓系肿瘤研究中的最新进展
Dharamveer Tatwavedi,Andrea Pellagatti,Jacqueline Boultwood
Dharamveer Tatwavedi
Acquired myeloid malignancies are a spectrum of clonal disorders known to be caused by sequential acquisition of genetic lesions in hematopoietic stem and progenitor cells, leading to their aberrant self-renewal and differentiation. The inc...
Site-specific crosslinking reveals Phosphofructokinase-L inhibition drives self-assembly and attenuation of protein interactions [0.03%]
位点特异性交联揭示了磷酸果糖激酶-L抑制驱动自组装和减弱蛋白质相互作用
Athira Sivadas,Eli Fritz McDonald,Sydney O Shuster et al.
Athira Sivadas et al.
Phosphofructokinase is the central enzyme in glycolysis and constitutes a highly regulated step. The liver isoform (PFKL) compartmentalizes during activation and inhibition in vitro and in vivo, respectively. Compartmentalized PFKL is hypot...
Steroidogenic Factor-1 form and function: From phospholipids to physiology [0.03%]
甾体生成因子-1的形式和功能：从磷脂到生理学
Alexis N Campbell,Woong Jae Choi,Ethan S Chi et al.
Alexis N Campbell et al.
Steroidogenic Factor-1 (SF-1, NR5A1) is a member of the nuclear receptor superfamily of ligand-regulated transcription factors, consisting of a DNA-binding domain (DBD) connected to a transcriptional regulatory ligand binding domain (LBD) v...
Phosphorylation impacts GLE1 nuclear localization and association with DDX1 [0.03%]
磷酸化影响GLE1的核定位及其与DDX1的相互作用
Manisha Sharma,Aaron C Mason,T Renee Dawson et al.
Manisha Sharma et al.
Gle1 regulates gene expression at multiple steps from transcription to mRNA export to translation under stressed and non-stressed conditions. To better understand Gle1 function in stressed human cells, specific antibodies were generated tha...