Refinement of amino-acid conformation vs. difference density maps in time-resolved serial femtosecond crystallography data analysis [0.03%]
基于时间分辨序列飞秒衍射数据的氨基酸构象精修与差值电子密度图计算
Meng Iao Fong,Yuhei Hosokawa,Lars Oliver Essen et al.
Meng Iao Fong et al.
Time-resolved crystallography is a revolutionary X-ray diffraction technique by which the structural features of short-lived, transient intermediates of in crystallo reactions can be elucidated. While visualizing time-dependent structural c...
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes [0.03%]
链球菌covRS突变的分子特征研究
Jarrad Pritchard,Emma-Jayne Proctor,Jiawa Wang et al.
Jarrad Pritchard et al.
Increasingly invasive Streptococcus pyogenes disease has been linked to the emergence of the M1UK lineage. Here, we used a murine infection model to investigate the ability of M1UK to accumulate mutations in the covRS operon which regulates...
Hyperosmotic stress-induced redistribution of pre-mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation [0.03%]
高渗压力诱导的前信使核糖核酸切割因子I亚单位的重新分布与多腺苷酸化选择位点变化有关联
Hitomi Soumiya,Masatake Osawa,Hidefumi Fukumitsu
Hitomi Soumiya
Alternative polyadenylation (APA) is an important mechanism of cellular stress response mediated in part by the cleavage factor Im (CFIm) complex. However, the spatiotemporal dynamics and regulatory activity of the mammalian CFIm complex du...
Time-restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance [0.03%]
结核分枝杆菌感染前限时进食减少组织CD4+T细胞但对细菌清除影响有限
Ashish Gupta,Nidhi Yadav,Subhasmita Das et al.
Ashish Gupta et al.
The effects of time-restricted feeding (TRF) on immune responses during bacterial infection are not well-studied. Here, we subjected mice (6-8 weeks, male) to 8 h of TRF for 30 days and then infected them with a low dose of Mycobacterium tu...
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins [0.03%]
autofluorescent蛋白的自溶酶体参与及其核转位评价
Keiichi Ikeda
Keiichi Ikeda
During the construction of control mCherry-labelled HeLa cells, we unexpectedly observed red fluorescence in cell nuclei and therefore investigated the mechanisms underlying the transport of fluorescent proteins (FPs) into the nuclei. We co...
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells [0.03%]
AMBRA1丢失激活黑色素瘤细胞中的MAPK和血管生成信号通路
Milad Ibrahim,Marco Corazzari,Iman Osman et al.
Milad Ibrahim et al.
The protein activating molecule in Beclin1-regulated autophagy1 (AMBRA1), discovered in 2007, is crucial for autophagy and plays roles in nervous system development, cell survival, and proliferation. Here, we investigated AMBRA1's involveme...
Hydrostatic pressure activates HIF-1α via β-catenin to promote stemness in breast cancer cells [0.03%]
乳腺癌细胞中静水压通过β-连环蛋白活化HIF-1α以促进干细胞特性
Da Zhai,Yong Xu,Jingjing Ma et al.
Da Zhai et al.
Tumor microenvironment plays a pivotal role in regulating the biological properties of cancer cells. Interstitial fluid hydrostatic pressure (HP) generally increases in malignant solid tumors. While our recent work has demonstrated that ele...
Guiding AlphaFold to predict how Munc13-1 opens Syntaxin-1 [0.03%]
引导AlphaFold预测Munc13-1如何打开Syntaxin-1
Madhurima Chattopadhyay,Junjie Xu,Josep Rizo
Madhurima Chattopadhyay
The SNARE proteins syntaxin-1, synaptobrevin, and SNAP-25 mediate neurotransmitter release by forming SNARE complexes that fuse synaptic vesicles with the plasma membrane. SNARE complex assembly is orchestrated by Munc18-1 and Munc13-1 thro...
Hyperactive ice-binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans [0.03%]
一种新型冰结合蛋白可稳定细胞膜并提高秀丽隐杆线虫抵抗脱水胁迫的耐受能力
Daiki Shimose,Kotaro Ozaki,Ryohei Kuriyama et al.
Daiki Shimose et al.
Ice-binding proteins (IBPs) are known to modulate ice growth and promote freezing tolerance and have recently attracted attention due to their cell-protective functions under freezing or low-temperature conditions. In this study, we demonst...
Petr Svoboda,Josef Pasulka
Petr Svoboda
RNA silencing pathways use small RNAs to guide sequence-specific repression of endogenous genes, mobile elements, and viruses. It has been 25 years since RNA interference (RNAi) was found in mouse oocytes and became the first identified sma...