Dose-dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction [0.03%]
剂量依赖性的过氧化氢在HepG2细胞中的肝毒性及其对CYP450诱导的调节作用
Maren Jinks,Garth L Maker,Emily C Davies et al.
Maren Jinks et al.
In vitro liver models combined with metabolomics approaches offer promising alternatives to animal testing in toxicology. In this study, we investigated concentration-dependent effects of hydrogen peroxide (H2O2) on the intra- and extracell...
How phagocytic cells kill bacteria: Lessons from a professional killer [0.03%]
吞噬细胞是如何杀死细菌的——来自一位专业杀手的启示
Otmane Lamrabet,Pierre Cosson
Otmane Lamrabet
Professional phagocytic cells such as neutrophils and macrophages, as well as free-living soil amoebae like Dictyostelium discoideum, employ evolutionarily conserved mechanisms to ingest and kill bacteria. Ingested bacteria are killed in ph...
MagmaFlow: A desktop platform for artificial intelligence-driven expression analysis [0.03%]
基于人工智能的表情分析桌面平台MagmaFlow
Carlos E Buss,Ao Li,Eduardo H Gilglioni et al.
Carlos E Buss et al.
While simplistic volcano plot visualizations of multi-omics changes can highlight the most critical genomic, transcriptomic, or proteomic features, integrative frameworks combining literature evidence, pathway associations, and functional a...
A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress [0.03%]
新的流动芯片结合多光子显微镜作为纵向三维剪切应力下组织钙化的成像协议
Vytautas Kučikas,Aaron D Morgan,Ignas Čiurlionis et al.
Vytautas Kučikas et al.
We present a novel miniaturized flow chip platform enabling thin-layer smooth muscle cell (SMC) culture, continuous tangential perfusion, dual-channel nutrient control, and longitudinal subcellular resolution 3-D imaging via multiphoton mic...
Yuya Ueno,Shota Yamazaki,Hiromichi Hoshina et al.
Yuya Ueno et al.
Various external and internal factors constantly induce DNA damage, with DNA double-strand breaks (DSBs) being the most severe form of damage. DSBs must be repaired rapidly, and failure to repair leads to the development of cancer and other...
Transcripts enriched in codons that trigger P-site tRNA-mediated mRNA decay possess stable mRNA [0.03%]
富含触发P位点tRNA介导的mRNA降解密码子的转录本具有稳定的mRNA
Rodolfo Lopes Carneiro,Fernando Lucas Palhano
Rodolfo Lopes Carneiro
Synonymous codon usage significantly influences mRNA stability in yeast by guiding mRNA decay during translation. The CCR4-NOT complex is central to this process, interacting with ribosomes when the A and E sites are unoccupied, a state tha...
Versatile vector tools for efficient protein screening across multiple expression systems [0.03%]
高效跨越多种表达系统的蛋白质筛选 versatile vector工具包
Zhimin Zhu,Yaqing Liu,Lei Qin et al.
Zhimin Zhu et al.
Heterologous protein expression is a cornerstone of biological research, but screening across different expression systems is often labor-intensive. We developed versatile vector tools for E. coli, insect, and mammalian cells, offering His,...
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education [0.03%]
途径与陷阱：生物医学科学教育中学生的体验定性研究
Olivia J Russell,Cassy Ross,Panagiota S Filippou et al.
Olivia J Russell et al.
Innovative pedagogies and support systems are critical for engaging diverse students in biochemistry and molecular biology education. This qualitative study explored biomedical science students' experiences of teaching, learning and support...
Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway [0.03%]
IGFBP4通过IGF1R/AKT途径对人前脂肪细胞增殖及分化的影响
Yujia Guo,Sandy Richter,Christoph Bach et al.
Yujia Guo et al.
Adipogenesis involves preadipocyte proliferation and differentiation into mature adipocytes, with dysregulation of this process being associated with changes in metabolic health. Here, we aimed to evaluate the effect of IGFBP4 knockdown (KD...
From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer [0.03%]
从能量供应到蛋白质合成：纳米管作为癌细胞间代谢合作的介质
Stanislava Martínková,Jan Trnka
Stanislava Martínková
Tunnelling nanotubes (TNTs) are thin intercellular membrane structures, which enable direct cytoplasmic communication between distant cells. Since their discovery two decades ago, TNTs have been identified in numerous physiological and path...