Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies [0.03%]
拟素复合物的纯化及相分离研究中MAP2的表达纯化
Bas Janssen,Robin Romein,Willy A M van den Berg et al.
Bas Janssen et al.
Eukaryotic cells compartmentalise to optimise their biochemistry. This occurs through membrane-surrounded compartments, but also through membraneless organelles (MLOs). Many MLOs arise from Liquid-Liquid Phase Separation (LLPS) of proteins....
A yeast model of 5-oxoproline accumulation reveals a general toleration to 5-oxoproline [0.03%]
一个关于5-氧脯氨酸积累的酵母模型揭示了对5-氧脯氨酸的耐受性
Pratiksha Dubey,Vaishnavi Sanjayan,Praveen Singh et al.
Pratiksha Dubey et al.
5-oxoproline (5-OP) or pyroglutamic acid is an intermediate in the degradation arc of the glutathione cycle. It is metabolized into glutamate through the action of the 5-oxoprolinase enzyme, the only enzyme known to act on this metabolite. ...
Optimizing photoexcitation conditions for time-resolved X-ray solution scattering experiments [0.03%]
用于时间分辨溶液散射实验的光激发条件优化
Matteo Levantino
Matteo Levantino
Investigating the dynamics of biological molecules in real time is crucial to understand their biological function. Time-resolved X-ray solution scattering (TR-XSS) is an experimental technique that allows one to follow large-scale conforma...
Structural studies and functional engineering of NanX: an anhydro-sialic acid transporter from Escherichia coli [0.03%]
枯基岩藻糖酸盐转运蛋白NanX的结构研究及功能改造
Michael C Newton-Vesty,Kelsi R Hall,Zachary D Tillett et al.
Michael C Newton-Vesty et al.
NanT and NanX are bacterial transporters that import the sialic acids, N-acetylneuraminate and 2,7-anhydro-n-acetylneuraminate, respectively. Here, we used complementary biophysical and computational approaches to structurally characterise ...
ATZ-1 promotes DNA replication efficiency to maintain normal meiotic function [0.03%]
ATZ-1促进DNA复制效率以维持正常的减数分裂功能
Taylin E Gourley,Luke W Molesworth,Nethma H Waduge et al.
Taylin E Gourley et al.
Precise coordination of DNA replication and meiotic progression is essential for germline genome stability, yet a complete understanding of this interplay remains unknown. In this study, we show that the absence of Abnormal Transition Zone ...
Delivery of DNA-encoded vaccines and proteins in mice using cutaneous suction-mediated transfection [0.03%]
利用皮肤吸吮介导的转染在小鼠中递送DNA编码型疫苗和蛋白质
Emran O Lallow,Isabel Brandtjen,Yaxin Mo et al.
Emran O Lallow et al.
A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction-based methods have demonstrated effectiveness in many animal models and have bee...
Treatment with KCL-286, a first-in-class retinoic acid receptor-β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease [0.03%]
第一种雷特酸受体β(RARβ)激动剂KCL-286治疗改善阿尔茨海默病小鼠模型的神经元DNA损伤和炎症
Natasha Hill,Hassan Yousef Othman AlMuallim,Elouisa Maddock et al.
Natasha Hill et al.
Alzheimer's disease (AD) is a complex, multifactorial neurodegenerative disorder for which effective disease-modifying therapies remain limited. Accumulation of neuronal DNA double-strand breaks (DSBs) is an early pathological event that co...
Importin 7 mediates the nuclear import of HIV-1 integrase via a specific interacting interface [0.03%]
进口蛋白7通过特异的相互作用介导HIV-1整合酶的核输入
Juana Bana,Avigail Yariv,Tal Oppenheim et al.
Juana Bana et al.
HIV-1 integrase (IN) must cross the nuclear envelope to access the host genome and catalyze viral DNA integration, a process that requires active nuclear import. Although recent work has established that the primary pathway for HIV-1 nuclea...
Malformin A1-mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy [0.03%]
畸形蛋白A1通过细胞焦亡和自噬诱导卵巢癌细胞毒性
Nada Abdullah Hassan,Ikram A Burney,Shika Hanif Malgundkar et al.
Nada Abdullah Hassan et al.
Cytoskeletal proteins play a crucial role in providing mechanical support and regulating key cellular processes such as cell proliferation, migration, and invasion. Cytoskeletal damage has been increasingly regarded as a contributing factor...
Evaluating the effect of γ-oryzanol on MASLD pathology using a medaka fish model [0.03%]
利用斑马鱼模型评估γ-稻醇对MASLD病理影响的研究
Yukako Ito,Haduki Asano,Ayano Ueki et al.
Yukako Ito et al.
Metabolic dysfunction-associated steatotic liver disease (MASLD) encompasses liver conditions not caused by alcohol, including metabolic dysfunction-associated steatohepatitis (MASH), which involves inflammation and fibrosis, with rising gl...