Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes [0.03%]
与小鼠睾丸中pachytene piRNA结合的MIWI内切核酸酶结构
Nicole Raad,Carmen Fernandez-Rodriguez,Radha Raman Pandey et al.
Nicole Raad et al.
PIWI-interacting RNAs (piRNAs) guide PIWI endoribonucleases to destroy transposon transcripts, ensuring animal fertility. Here, we report the cryo-electron microscopy structure of the MIWI-pachytene piRNA complex isolated from mouse testes....
TOF-probe-based mass cytometry reveals individual protease activity as an important driver of immune cell differentiation and function [0.03%]
基于时间-of-flight探针的质谱流式细胞术揭示了个别蛋白酶活性是免疫细胞分化和功能的重要驱动因素
Katarzyna Groborz,Marcin Poręba,Marcin Drąg et al.
Katarzyna Groborz et al.
Quantifying enzymatic activity at the single-cell level remains challenging. Building on previous development of metal-tagged activity-based probes (time-of-flight [TOF]-probes) compatible with mass cytometry, we adapt this technology to qu...
Derek H Janssens,Christine A Codomo,Dominik J Otto et al.
Derek H Janssens et al.
Promoter-proximal pausing of RNA polymerase II (Pol II) primes genes for rapid activation, yet how Pol II dynamics are temporally organized in adult stem cells to enable fast and flexible responses to environmental cues remain unknown. To a...
METTL3-mediated m6A on nascent RNA coordinates translational and transcriptional programs to activate the NLRP3 inflammasome in macrophages [0.03%]
巨噬细胞中METTL3介导的新生RNA上的m6A调控翻译和转录程序以激活NLRP3炎性小体
Jie Fu,Xin Zong,Hong Zhang et al.
Jie Fu et al.
NLRP3 inflammasome activation requires both transcriptional priming and complex assembly, but how RNA m6A methylation coordinates these steps remains unclear. Here, we show that m6A levels increase during macrophage NLRP3 inflammasome activ...
IRF3 attenuates hypoxia signaling by retaining HIF-α in the cytoplasm [0.03%]
IRF3通过在细胞质中滞留HIF-α来抑制缺氧信号响应
Hongyan Deng,Shuke Jia,Chunchun Zhu et al.
Hongyan Deng et al.
Interferon regulatory factor 3 (IRF3) functions as a key transcription factor in the innate antiviral immune response, which depends on its nuclear localization. However, its function in the cytoplasm during non-infection states remains lar...
Rumen microbiome biogeography and ventral epithelial architecture in three ruminant species [0.03%]
三种反刍动物瘤胃微生物群生物地理学和腹侧上皮结构
Jianan Sang,Songze Li,Chao Xu et al.
Jianan Sang et al.
Ruminants thrive in diverse ecosystems by leveraging their rumen microbiome to ferment fibrous plants. However, the spatial biogeography of rumen microbiome and the genetic diversity of the ventral rumen epithelium remain unknown. Here, we ...
Prior high fiber intake impinges on the cellular responses of mesenteric adipose and intestinal tissues to subsequent high fat feeding [0.03%]
高纤维摄入通过影响肠道和肠系膜脂肪组织的细胞反应来对抗随后的高脂饮食引起的肥胖及其相关代谢损伤
Zhi Peng,Houyu Zhang,Yifei Ding et al.
Zhi Peng et al.
While high-fiber diets (HfiDs) promote weight loss, their long-term efficacy is limited by rapid weight regain upon returning to high-fat diets (HFDs). Using C57BL/6J mice in diet-switching paradigms, we characterized tissue-specific respon...
N(6)-methyladenosine modification of RNA is regulated by senataxin and E6 to control HPV replication [0.03%]
SENATAXIN 和 E6 通过调节 RNA 上的 N6-甲基腺苷修饰来控制 HPV 复制
Conor W Templeton,Laimonis A Laimins
Conor W Templeton
The levels of N6-methyladenosine (m6A)-modified RNAs are reduced within cells with high-risk human papillomaviruses (HPVs) in comparison to normal cells. These reduced m6A levels stabilize RNA:DNA hybrids (R-loops), whose regulation is crit...
Exploiting the polypharmacology of alectinib for synergistic RNA splicing disruption with RBM39 degraders [0.03%]
利用alectinib的多靶点特性与RBM39降解剂协同干扰RNA剪切
Yurui Ma,Evon Poon,Chenchen Jin et al.
Yurui Ma et al.
Precise control of pre-mRNA splicing is vital for transcriptome integrity, and its disruption is an emerging cancer vulnerability. Here, we use indisulam to degrade RBM39 and show that the clinical ALK inhibitor alectinib can be repurposed ...
Genetic identification of mouse trigeminal afferents responsible for mechanical allodynia [0.03%]
小鼠三叉神经末梢的基因鉴别负责机械性异常疼痛感
Tingting Li,Hyeonwi Son,Vipin Arora et al.
Tingting Li et al.
The identities of primary afferents transducing mechanical allodynia following nerve injury remain unclear. We genetically label brushing-activated (BA) trigeminal ganglia (TG) neurons using FosCreER mice with trigeminal nerve injury (TNI)....