Multi-omics analysis reveals NSUN2-mediated m5C methylation of DOCK9 regulating vascular stability in brain arteriovenous malformations [0.03%]
多组学分析揭示NSUN2介导的DOCK9 mRNA N端m5C甲基化通过调控血管稳定性参与脑动静脉畸形发病机制中的作用
Ganglei Li,Hongfei Zhang,Biyun Wang et al.
Ganglei Li et al.
RNA 5-methylcytosine (m5C) methylation is a key post-transcriptional modification, yet its role in vascular stability remains unclear. Through integrative m5C-RNA immunoprecipitation and transcriptomic, proteomic, and single-cell analyses o...
Phase separation of heterochromatin protein 1 regulates virulence gene expression in Plasmodium falciparum [0.03%]
异染色质蛋白1的相分离调控恶性疟原虫的毒力基因表达
D V Mamatharani,Devatrisha Purkayastha,Igor Niederwieser et al.
D V Mamatharani et al.
Plasmodium falciparum malaria relies on antigenic variation mediated by mutually exclusive expression of var genes. Repressed var genes cluster together and are bound by PfHP1 (Plasmodium falciparum heterochromatin protein 1), but the regul...
A multifaceted kinase keeps molecular motors in place for faithful cell division [0.03%]
多功能激酶使分子马达就位以确保细胞分裂的保真度
Paola Ghezzi,Marina Mapelli
Paola Ghezzi
During cell division, faithful chromosome segregation is ensured by the mitotic spindle. van Toorn et al.1 uncovered that Cdk1-mediated phosphorylation of the dynein-activating adaptor NuMA promotes the timely assembly of dynein/dynactin/Nu...
The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37 [0.03%]
CAG复杂重复序列插入Alu元件并激活脊髓小脑共济失调37型神经发育增强子
Joana R Loureiro,Ana F Castro,Ana S Figueiredo et al.
Joana R Loureiro et al.
Alu elements are evolutionarily very old primate-specific interspersed repeat elements that constitute ∼11% of the human genome. They are a source of short tandem repeats (STRs), which often expand in size and cause inherited neuromuscular...
IL-4 treatment induces apoptosis of blood monocytes and proliferation of recruited injury-associated macrophages to resolve liver injury [0.03%]
IL-4治疗通过促进血液单核细胞凋亡和募集损伤相关性巨噬细胞的增殖来修复肝脏损伤
Ruairi W Lynch,Pieter A Louwe,Liesbet Martens et al.
Ruairi W Lynch et al.
IL-4 can have significant therapeutic benefit in many injury settings, but its mechanism of action is unclear. Using a model of carbon tetrachloride (CCl4)-mediated acute liver injury, we find that exogenous IL-4 causes a dramatic shift fro...
An accessory orthosteric ligand binding site in the (α3)3(β4)2 nicotinic acetylcholine receptor regulates channel activation [0.03%]
(α3)3(β4)2烟碱乙酰胆碱受体的一个辅助变构配体结合位点调节通道激活
Gauri C Kulkarni,Megan J Jurek,Andrew P Riley et al.
Gauri C Kulkarni et al.
The α3β4 nicotinic acetylcholine receptor (nAChR) is a heteropentameric ligand-gated channel whose distribution includes reward circuits of the brain, where it regulates expression of drug withdrawal symptoms. α3β4 nAChRs form two stoic...
Distinct synaptic mechanisms underlie NRXN1 variant and disorder background-dependent phenotypes in iPSC-derived neurons [0.03%]
NRXN1变异体和疾病背景依赖性表型在诱导多能干细胞来源的神经元中的不同突触机制
Jay English,Danny McSweeney,Jinghui Geng et al.
Jay English et al.
Copy-number deletions in the 2p16.3/NRXN1 locus confer genetic risk for autism spectrum disorder (ASD) and schizophrenia (SCZ). Prior studies showed that heterozygous NRXN1 deletions reduce excitatory synaptic transmission in human induced ...
Deubiquitinating enzyme MINDY2 regulates TNF-α-induced cell death by targeting RIPK1 [0.03%]
脱泛素酶MINDY2通过靶向RIPK1调控TNF-α诱导的细胞死亡
Wenyang Huang,Yushi Chen,Danni Chen et al.
Wenyang Huang et al.
Ubiquitination plays a crucial role in the tumor necrosis factor (TNF)-α signaling pathway. To identify mechanisms by which ubiquitination regulates TNF-α signaling, we perform a screen using a gene expression library of ubiquitination-mo...
In vivo evolution of antibody CR3022 expands cross-neutralization of SARS-CoV-2 variants and informs pan-sarbecovirus immunity [0.03%]
体内抗体CR3022的进化可扩展其对SARS-CoV-2变异株的交叉中和并为广谱sarbecovirus免疫提供信息
Yanbin Fu,Ziqi Feng,Steven A Erickson et al.
Yanbin Fu et al.
The epitope that monoclonal CR3022 binds to represents a promising target for broad protection against a wide range of human and zoonotic coronaviruses. We develop a powerful model to evaluate antibody affinity maturation in vivo using immu...
B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection [0.03%]
持续性淋巴细囊炎病毒（LCMV）感染期间，I型干扰素信号传导损害模型抗原的抗体反应
Xavier Laulhé,Yasmine Adda-Bouchard,Guillaume Lopez et al.
Xavier Laulhé et al.
Affinity maturation and vaccine efficacy are compromised during chronic viral infections; however, underlying mechanisms remain unclear. Using the LCMV Cl13 model, we show that type I interferon (IFN-I) signaling in B cells plays a central ...