Long-read Sequencing: from Complete Molecules to Context-resolved Biology [0.03%]
长读测序技术：从全长分子到背景解析生物学
Wanlu Liu,Hui Shen,Yi Xing
Wanlu Liu
A Cattle BodyMap of Transcriptome across 52 Tissues and 3 Developmental Stages Reveals New Genetic Insights into Beef Production Traits [0.03%]
覆盖52种组织和3个发育阶段的肉牛转录组身体图谱揭示了新的影响牛肉生产性状的遗传规律
Wentao Cai,Yapeng Zhang,Lei Xu et al.
Wentao Cai et al.
Cattle are important livestock that provide essential meat and milk resources. However, a comprehensive analysis of gene expression, alternative splicing (AS), and RNA editing across various organs and developmental stages in cattle has not...
Xiang Jennie Li,Careen Foord,Andrey D Prjibelski et al.
Xiang Jennie Li et al.
The vast majority of multi-exonic genes are alternatively spliced, generating diverse and cell-type-specific isoforms exhibiting functional differences. To better capture this heterogeneity using single-cell long-read sequencing data, we pr...
Decoding RNA N6-Methyladenosine Methylome of Wheat Using Machine Learning and Nanopore Direct RNA Sequencing [0.03%]
基于机器学习和纳米孔直接RNA测序的小麦RNA N6-甲基腺苷甲基组解析
Minggui Song,Jing Yang,Songyu Liu et al.
Minggui Song et al.
Nanopore direct RNA sequencing (DRS) enables transcriptome-wide detection of N6-methyladenosine (m6A), a pivotal RNA modification that regulates plant growth, development, and stress responses, at single-base resolution. However, existing N...
Tranquillyzer: A Neural Network Framework for Long-read Annotation and Demultiplexing [0.03%]
基于神经网络的长读段注释和解复用框架tranquillyzer
Ayush Semwal,Jacob Morrison,Ian Beddows et al.
Ayush Semwal et al.
Long-read single-cell RNA sequencing enables full-length transcript profiling but remains limited by challenges in interpreting structurally complex sequencing reads. High error rates, heterogeneous library designs, and frequent molecular a...
Advancing Functional Transcriptomics in Zebrafish with High-accuracy Full-length RNA Sequencing [0.03%]
基于高精度全长转录组的斑马鱼功能基因研究及应用
Monika Kwiatkowska,Tomasz Mądry,Marta Blangiewicz et al.
Monika Kwiatkowska et al.
Zebrafish (Danio rerio) is a powerful vertebrate model organism with strong genetic and physiological similarity to humans, yet its use in large-scale transcriptomic research remains constrained by incomplete gene annotations, inefficient r...
NanoRAPID: A Deep Learning-based Framework for Single-molecule RNA Structure Analysis Using Nanopore Direct RNA Sequencing [0.03%]
NanoRAPID：一种基于深度学习的框架，用于使用纳米孔直接RNA测序分析单分子RNA结构
Ze-Hui Ren,Hong-Xuan Chen,Ying-Yuan Xie et al.
Ze-Hui Ren et al.
RNA structure is fundamental to its diverse biological functions. Current chemical probing methods, coupled with next-generation sequencing, offer insights into RNA secondary structure but are limited by indirect readouts and averaging of m...
Single-cell Multiomic and Spatiotemporal Dissection of the Liver Circadian Clock [0.03%]
单细胞多组学和时空分析肝脏昼夜节律钟表
Chun Yip Tong,Changhao Li,Audrey Jacq et al.
Chun Yip Tong et al.
Circadian rhythms are remarkably widespread across most organisms, regulating hormonal, metabolic, physiological, and behavioral oscillations through molecular clocks that orchestrate the rhythmic expression of thousands of genes. Here, we ...
π-HelixNovo2: Making Accurate Online De Novo Peptide Sequencing Available to All [0.03%]
π-螺旋Novo2：使所有用户都能获得准确的在线从头肽测序技术
Tingpeng Yang,Tianze Ling,Boyan Sun et al.
Tingpeng Yang et al.
De novo peptide sequencing, the mainstream technique for identifying novel peptides, has recently seen remarkable improvements due to deep learning approaches. However, existing models struggle to effectively enhance the encoding of mass sp...
CTSC-RAB38 Potentiates Responsiveness to PD-1 Blockade in Esophageal Squamous Cell Carcinoma [0.03%]
CTSC-RAB38增强食管鳞状细胞癌对PD-1阻断的反应性
Wenyan Gao,Xiao Yu,Wenqing Wei et al.
Wenyan Gao et al.
Immune checkpoint blockade (ICB) shows promise in treating locally advanced esophageal squamous cell carcinoma (ESCC); however, identifying biomarkers for predicting efficacy remains a challenge. Chimeric RNAs offer a potential solution for...