Haruki Nakamura
Haruki Nakamura
Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system [0.03%]
使用多电极阵列系统对高钾血症心肌细胞进行电生理分析
Kentaro Kito,Masahito Hayashi,Tomoyuki Kaneko
Kentaro Kito
The action potential of cardiomyocytes is controlled by electrolytes in serum such as Na+, K+ and Ca2+. Hyperkalemia, which refers to an abnormally high concentration of K+ in the blood, can induce lethal arrythmia. In this study, the extra...
Monitoring of enzymatic cleavage reaction of GST-fusion protein on biolayer interferometry sensor [0.03%]
基于生物层干涉测量传感器的GST融合蛋白酶切反应监测
Sena Tarumoto,Sei Inoue,Rina Yanagimoto et al.
Sena Tarumoto et al.
Biolayer interferometry (BLI) is an optical sensor-based analytical method primarily used for analyzing interactions between biomolecules. In this study, we explored the application of BLI to observe the cleavage reaction of glutathione S-t...
Four-color single-molecule imaging system for tracking GPCR dynamics with fluorescent HiBiT peptide [0.03%]
一种用于利用荧光HiBiT肽追踪GPCR动力学的四色单分子成像系统
Toshiki Yoda,Yasushi Sako,Asuka Inoue et al.
Toshiki Yoda et al.
Single-molecule imaging provides information on diffusion dynamics, oligomerization, and protein-protein interactions in living cells. To simultaneously monitor different types of proteins at the single-molecule level, orthogonal fluorescen...
Data-driven score tuning for ChooseLD: A structure-based drug design algorithm with empirical scoring and evaluation of ligand-protein docking predictability [0.03%]
基于数据的评分调整：一种经验性药物设计算法及其配体-蛋白质对接预测能力评估
Akihiro Masuda,Daichi Sadato,Mitsuo Iwadate
Akihiro Masuda
Computerized molecular docking methodologies are pivotal in in-silico screening, a crucial facet of modern drug design. ChooseLD, a docking simulation software, combines structure- and ligand-based drug design methods with empirical scoring...
Function of nodal cilia in left-right determination: Mechanical regulation in initiation of symmetry breaking [0.03%]
结节纤毛在左右对称性决定中的功能：打破对称性的力学调控机制
Takanobu A Katoh
Takanobu A Katoh
Visceral organs in vertebrates are arranged with left-right asymmetry; for example, the heart is located on the left side of the body. Cilia at the node of mouse early embryos play an essential role in determining this left-right asymmetry....
Chemical tongues as multipurpose bioanalytical tools for the characterization of complex biological samples [0.03%]
化学味觉传感器作为复杂生物样本特征分析的多功能生化分析工具
Shunsuke Tomita,Hiroka Sugai
Shunsuke Tomita
Chemical tongues are emerging powerful bioanalytical tools that mimic the mechanism of the human taste system to recognize the comprehensive characteristics of complex biological samples. By using an array of chromogenic or fluorogenic prob...
Unraveling the fastest myosin: Discovery history and structure-function relationships of algae Chara myosin XI [0.03%]
解开最快的肌球蛋白：水藻Chara myosin XI的发现史及其结构功能关系
Kohji Ito,Takeshi Haraguchi
Kohji Ito
Plant myosins have higher velocities than animal myosins. Among them, myosins in freshwater algae of the genus Chara have extremely high velocities. We have biochemically studied myosins that perform high-speed movements in the alga Chara. ...
Internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography [0.03%]
利用负染色电子层析术分析Mycoplasma mobile的运动装置的内部结构
Minoru Fukushima,Takuma Toyonaga,Yuhei O Tahara et al.
Minoru Fukushima et al.
Mycoplasma mobile is a parasitic bacterium that forms gliding machinery on the cell pole and glides on a solid surface in the direction of the cell pole. The gliding machinery consists of both internal and surface structures. The internal s...
Optimizing tumor treating fields for blood cancer therapy: Analysis of electric field distribution and dose density [0.03%]
用于血液癌症治疗的肿瘤治疗场优化：电场分布和剂量密度分析
Nasori Nasori,Miftakhul Firdhaus,Ulya Farahdina et al.
Nasori Nasori et al.
Blood cancer is a condition in which white blood cells grow uncontrollably. Tumor treating fields (TTF) are a modality of cancer treatment that utilizes electric fields to target malignant cells. To optimize the efficacy of TTF, it is neces...