Chlamylipo, a Chlamydomonas-in-liposome microswimmer: Self-propelled swimming and associated lipid membrane flow [0.03%]
一种新型膜泳体“Chlamylipo”：“绿藻-脂质体复合物”的构建、推进及膜流观测研究
Shunsuke Shiomi,Koichiro Akiyama,Hiromasa Shiraiwa et al.
Shunsuke Shiomi et al.
Developing active transport systems for micro cargo delivery is challenging because it requires overcoming the constraints imposed by low Reynolds numbers. We developed a bio-hybrid micro-swimmer, "Chlamylipo" consisting of the green alga C...
Yoshifumi Nishimura
Yoshifumi Nishimura
Chromatin in the eukaryotic nucleus is organized into transcriptionally active euchromatin and transcriptionally silent heterochromatin. Its fundamental unit, the nucleosome, consists of two copies each of histones H2A, H2B, H3, and H4 wrap...
Development of ultrafast single fluorescent-molecule imaging and its application to unravel plasma membrane structure and function in live cells [0.03%]
超快单分子荧光成像技术的发展及其在活细胞中解析质膜结构与功能方面的应用
Takahiro Fujiwara,Akihiro Kusumi
Takahiro Fujiwara
Single-molecule imaging in live cells now plays key roles in elucidating molecular dynamics and interactions. However, the imaging time resolution has remained limited, despite its critical importance for precisely capturing molecular event...
Live imaging of bacterial actin MreBs from Spiroplasma causing helicity switching of a minimal synthetic cell [0.03%]
螺旋体致病菌细菌肌动蛋白MreB活细胞成像及其最小合成细胞的旋转方向切换现象
Yoshiki Tanaka,Hana Kiyama,Yusuke V Morimoto et al.
Yoshiki Tanaka et al.
Spiroplasma swim by switching the handedness of their helical bodies between right- and left-handed. Helicity formation and switching can be reconstituted in an immotile minimal synthetic bacterium, JCVI-syn3B by introducing a pair of bacte...
Cooperative and divergent properties of bacterial actin isoforms in Spiroplasma swimming [0.03%]
Spiroplasma螺旋游动中细菌肌动蛋白同工型的协同和发散性质
Daichi Takahashi,Makoto Miyata,Ikuko Fujiwara
Daichi Takahashi
The cytoskeleton, comprising intracellular filamentous structures composed of polymerized proteins, is crucial for the survival of both eukaryotes and prokaryotes. Although bacterial cytoskeletal proteins have diverged, they generally do no...
Oligo DNA-based quantum dot (QD) single-particle tracking for multicolor single-molecule imaging [0.03%]
基于寡核苷酸DNA的量子点(QD)单颗粒追踪技术用于多色单分子成像研究
Shigeo Sakuragi,Naoki Kato,Tomoya Uchida et al.
Shigeo Sakuragi et al.
Quantum dot single-particle tracking (QD-SPT), a powerful tool for analyzing membrane domains that are critical to various cellular processes, is widely used in membrane molecular dynamics research. QDs, which possess both a broad excitatio...
Substrate elasticity controls fibroblast motility on non-oxidized PDMS under weak adhesion [0.03%]
基质弹性在弱黏附下非氧化PDMS上成纤维细胞迁移的作用
Arata Nagai,Kyotaro Kanazashi,Hiromu Kuwabara et al.
Arata Nagai et al.
Cardiac fibroblasts play a pivotal role in heart tissue dynamics, responding to mechanical and interfacial cues in their microenvironment. This study examines how substrate stiffness and surface condition influence the motility and morpholo...
Hisashi Okumura,Linh Truong Hoai,Pornthep Sompornpisut et al.
Hisashi Okumura et al.
Proteins can aggregate to form amyloid fibrils, which are associated with a group of diseases collectively known as amyloidoses. Molecular dynamics simulations are widely used to study protein aggregation; however, it remains computationall...
Revisiting hydrolytic mechanisms of bacterial RNase HI: Towards clarification of single mobile Mg2+ action in nuclease reactions [0.03%]
细菌RNase HI水解机制的再研究：朝向澄清核酸酶反应中单一可移动Mg2+的作用方向
Kosuke Morikawa,Takuji Oyama,Masayuki Oda
Kosuke Morikawa
Cellular nuclei contain various nucleases that play major roles in gene expression and regulation. They are classified into various categories based on their three-dimensional structures and specificities. Since the discovery of ribozyme in...
NMR evidence for residue-based LFER relationship in two-state folding-unfolding equilibrium of the spectrin SH3 domain in acidic solutions [0.03%]
酸性溶液中spectrin的SH3结构域二级结构变化的核磁共振研究
Seiichiro Hayashi,Daisuke Fujinami,Daisuke Kohda
Seiichiro Hayashi
The conformational change of a protein molecule is described by the single equilibrium constant K and the single rate constants, k (forward) and k' (reverse). The underlying assumption is that all amino acid residues undergo state changes w...