NMR evidence for residue-based LFER relationship in two-state folding-unfolding equilibrium of the spectrin SH3 domain in acidic solutions [0.03%]
酸性溶液中spectrin的SH3结构域二级结构变化的核磁共振研究
Seiichiro Hayashi,Daisuke Fujinami,Daisuke Kohda
Seiichiro Hayashi
The conformational change of a protein molecule is described by the single equilibrium constant K and the single rate constants, k (forward) and k' (reverse). The underlying assumption is that all amino acid residues undergo state changes w...
Comparative evaluation of electrode configuration for optimizing positive dielectrophoresis trapping of CCRF-CEM cells [0.03%]
用于优化CCRF-CEM细胞正介电泳捕获的电极结构比较研究
Miftakhul Firdhaus,Ulya Farahdina,Nasori Nasori et al.
Miftakhul Firdhaus et al.
Dielectrophoresis (DEP) is a promising label-free technique for bioparticle manipulation, offering significant potential for diagnostic application such as isolation and trapping of cancerous CCRF-CEM cells. The efficacy of DEP trapping is ...
Mechanistic insights into a cold-adapted glucokinase with high thermal stability were revealed by site-directed spin labeling ESR [0.03%]
位点定向自旋标记EPR揭示冷适应且热稳定的己糖激酶的机理见解
Akane Yato,Masaki Horitani
Akane Yato
The structural flexibility of enzymes plays an essential role in determining their catalytic efficiency and thermal stability. Cold-adapted enzymes are typically highly flexible, resulting in high catalytic activity but low stability. Gluco...
The Norway-Japan bilateral symposium: Dialogue in biophysics beginning in Nara [0.03%]
挪威和日本双边生物物理研讨会——奈良对话开始了
Yoshihiko Furuike,Oliver Vanderpoorten
Yoshihiko Furuike
Hiroshi Koyama,Yasuhiro Inoue
Hiroshi Koyama
In morphogenesis, mechanical forces are the fundamental drivers of tissue deformation. To elucidate the principles governing this process, a thorough understanding of these forces is indispensable, and mathematical models are invaluable too...
Mirror life at the crossroads: Report on the symposia in Japan for discussion toward mirror life synthesis [0.03%]
镜中的十字路口：关于在日本讨论合成镜生命的一系列专题报告会
Kei Fujiwara,Wataru Aoki,Chikara Furusawa et al.
Kei Fujiwara et al.
The synthesis of mirror life, an organism composed entirely of the mirror-image counterparts of naturally occurring biomolecules, may be a topic for science fiction today but is suggested to be a reality in the future. Scientists called for...
Liquid-liquid phase separation and its regulation of the BACH2 intrinsically disordered region [0.03%]
液体液相分离及其对BACH2内在无序区域的调节作用
Hironori Hayashi,Miyuki Kato-Murayama,Takeshi Kurasawa et al.
Hironori Hayashi et al.
Intrinsically disordered regions (IDRs) are vital for several cellular processes. They play a significant role in liquid-liquid phase separation (LLPS). LLPS enhances reaction efficiency by locally concentrating proteins and nucleic acids. ...
Resurrecting lost ancient myoglobin proteins to elucidate mechanisms of diving adaptation in mammals [0.03%]
复活古代丢失的肌红蛋白蛋白质以阐释哺乳动物潜水适应机制
Hiroshi Imamura,Yasuhiro Isogai,Tsuyoshi Shirai
Hiroshi Imamura
Data science methodologies can be applied to "molecular archeology." By statistically inferring ancestral gene or protein sequences from a molecular phylogeny, researchers can recreate ancient molecules for laboratory experiments, allowing ...
Development of electromagnetic tweezers for single-molecule energetics of kinesin [0.03%]
用于单分子层析中动力蛋白能量研究的电磁镊子的发展
Haruyuki Fukuzawa,Yuga Ihara,Masato Sawa et al.
Haruyuki Fukuzawa et al.
Optical tweezers have enabled single-molecule measurements of kinesin mechanics and energetics but face challenges for in vivo applications due to photothermal effects and refractive-index-dependent force calibration. Here, we developed ele...
Analysis of metabolic stability under environmental perturbations using a kinetic model [0.03%]
基于动力学模型的环境扰动下代谢稳定性分析
Atsuki Hishida,Yusuke Himeoka,Chikara Furusawa
Atsuki Hishida
Cells robustly maintain metabolic functions despite environmental fluctuations that broadly alter reaction kinetics. However, kinetic models of cellular metabolism often exhibit fragility, losing stability with minor parameter changes. This...