Kazuho Yoshida,Takahiro Yamashita,Kengo Sasaki et al.
Kazuho Yoshida et al.
We previously showed that the chimeric proteins of microbial rhodopsins, such as light-driven proton pump bacteriorhodopsin (BR) and Gloeobacter rhodopsin (GR) that contain cytoplasmic loops of bovine rhodopsin, are able to activate Gt prot...
Analysis of the GTPase motif of FlhF in the control of the number and location of polar flagella in Vibrio alginolyticus [0.03%]
副溶血弧菌FlhF的GTP酶基序对极性鞭毛数量和定位调控机制的研究分析
Shota Kondo,Michio Homma,Seiji Kojima
Shota Kondo
Vibrio alginolyticus normally has a single polar flagellum whose number and placement are regulated positively by FlhF. FlhF is a GTPase and homolog of a signal recognition particle (SRP) protein called Ffh and SRP receptor FtsY. FlhF is lo...
Analysis of an ATP-induced conformational transition of ABC transporter MsbA using a coarse-grained model [0.03%]
基于粗粒化模型的ABC转运蛋白MsbA的ATP诱导构象变化分析研究
Naoki Arai,Tadaomi Furuta,Minoru Sakurai
Naoki Arai
Upon the binding of ATP molecules to nucleotide binding domains (NBDs), ATP-binding cassette (ABC) exporters undergo a conformational transition from an inward-facing (IF) to an outward-facing (OF) state. This molecular event is a typical e...
Computational and theoretical approaches for studies of a lipid recognition protein on biological membranes [0.03%]
用于研究生物膜上脂质识别蛋白的计算和理论方法
Eiji Yamamoto
Eiji Yamamoto
Many cellular functions, including cell signaling and related events, are regulated by the association of peripheral membrane proteins (PMPs) with biological membranes containing anionic lipids, e.g., phosphatidylinositol phosphate (PIP). T...
Toshifumi Kumai
Toshifumi Kumai
It is established knowledge that the action potential event of nerves is formed by the combination of a phasic inward Na+ current and a following outward K+ current which increases gradually. These changes in current are commonly referred t...
The change of picrotoxin-induced epileptiform discharges to the beta oscillation by carbachol in rat hippocampal slices [0.03%]
卡巴胆碱诱发河豚毒诱导的海马脑片癫痫样放电模式向β振荡模式转变
Ayumi Hashimoto,Toyohiro Sawada,Kiyohisa Natsume
Ayumi Hashimoto
The study aimed to determine whether and how the activation of the acetylcholine receptor affects epileptiform discharges in the CA3 region in a rat hippocampus. Picrotoxin (100 μM), a GABAA receptor antagonist, was applied to a hippocampa...
High-speed atomic force microscopy imaging of live mammalian cells [0.03%]
高速原子力显微镜实时观察活体哺乳动物细胞动态过程研究
Mikihiro Shibata,Hiroki Watanabe,Takayuki Uchihashi et al.
Mikihiro Shibata et al.
Direct imaging of morphological dynamics of live mammalian cells with nanometer resolution under physiological conditions is highly expected, but yet challenging. High-speed atomic force microscopy (HS-AFM) is a unique technique for capturi...
The use of a genetically encoded molecular crowding sensor in various biological phenomena [0.03%]
基因编码分子拥挤传感器在各种生物学现象中的应用
Hiroaki Machiyama,Takamitsu J Morikawa,Kazuko Okamoto et al.
Hiroaki Machiyama et al.
We evaluated usability of a previously developed genetically encoded molecular crowding sensor in various biological phenomena. Molecular crowding refers to intracellular regions that are occupied more by proteins and nucleotides than by wa...
Demonstration of correlative atomic force and transmission electron microscopy using actin cytoskeleton [0.03%]
利用原子力显微镜和透射电子显微镜观测细胞骨架中的肌动蛋白相关实验
Yutaro Yamada,Hiroki Konno,Katsuya Shimabukuro
Yutaro Yamada
In this study, we present a new technique called correlative atomic force and transmission electron microscopy (correlative AFM/TEM) in which a targeted region of a sample can be observed under AFM and TEM. The ultimate goal of developing t...
Monte Carlo simulation of a statistical mechanical model of multiple protein sequence alignment [0.03%]
多重蛋白质序列比对的统计力学模型的蒙特卡罗模拟
Akira R Kinjo
Akira R Kinjo
A grand canonical Monte Carlo (MC) algorithm is presented for studying the lattice gas model (LGM) of multiple protein sequence alignment, which coherently combines long-range interactions and variable-length insertions. MC simulations are ...