Masayuki Hayakawa,Ibuki Kawamata
Masayuki Hayakawa
Origin of the unique topology of the triangular water cluster in Rubrobacter xylanophilus rhodopsin [0.03%]
Rubrobacter xylanophilus视紫红质中三角形水合物团簇的特殊拓扑结构的起源
Tomoyasu Noji,Masaki Tsujimura,Keisuke Saito et al.
Tomoyasu Noji et al.
The crystal structure of Rubrobacter xylanophilus rhodopsin (RxR) reveals a triangular cluster of three water molecules (W413, W415, and W419) at the extracellular proton-release site, near Glu187 and Glu197. Using a quantum mechanical/mole...
Abhishek Mallick,Bishal Paul,Anirudhha Roy et al.
Abhishek Mallick et al.
Quality assessment and characterization of liquid milk by means of electronic sensor remains an intensive area of research. In this work, cylinder-in-cylinder type sample holder is fabricated to measure the bioimpedance of milk as a functio...
Polymerization and stability of actin conjugated with polyethylene glycol [0.03%]
聚乙二醇化肌动蛋白的聚合与稳定性研究
Masaya Sagara,Kuniyuki Hatori
Masaya Sagara
This study aimed to elucidate the impact of polyethylene glycol (PEG) conjugation on protein-protein interactions by investigating the properties of PEG-conjugated actin (PEG-actin). Various PEG molecules were covalently bound to actin mono...
HulaChrimson: A Chrimson-like cation channelrhodopsin discovered using freshwater metatranscriptomics from Lake Hula [0.03%]
取自胡拉湖淡水元转录组的类似阳离子通道视紫红质的HulaChrimson的发现
Hiroto Takahashi,Shunki Takaramoto,Takashi Nagata et al.
Hiroto Takahashi et al.
Chrimson is cation-conducting channelrhodopsin (CCR) with the most red-shifted absorption spectrum, rendering itself as one of the most promising optogenetic tools. However, the molecular mechanisms underlying its red-shifted absorption hav...
Protein-ligand affinity prediction via Jensen-Shannon divergence of molecular dynamics simulation trajectories [0.03%]
基于分子动力学模拟轨迹的詹森香农散度的蛋白质-配体亲和力预测方法研究
Kodai Igarashi,Masahito Ohue
Kodai Igarashi
Predicting the binding affinity between proteins and ligands is a critical task in drug discovery. Although various computational methods have been proposed to estimate ligand target affinity, the method of Yasuda et al. (2022) ranks affini...
Cytoskeleton as a generator of characteristic physical properties of plant cells: 'cell wall,' 'large vacuole,' and 'cytoplasmic streaming' [0.03%]
细胞骨架作为产生植物细胞特征物理性质的生成器：细胞壁、大液泡和胞质流
Amari Toshiki,Noriko Nagata,Motoki Tominaga et al.
Amari Toshiki et al.
As sessile organisms, plants must constantly adapt to ever-changing environmental conditions. To survive in their habitats, plants have evolved characteristic cellular features that make the cells rigid yet dynamic. These include the cell w...
A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality [0.03%]
一种提高完整性和功能的线粒体分离和冻存方法
Arima Okutani,Jannatul Naima,Asaka Ogihara et al.
Arima Okutani et al.
Mitochondria isolated from cells are essential tools in biological research. However, many mitochondria are often damaged during the isolation process. Although cryopreservation can greatly improve the usability of isolated mitochondria, it...
A virtual system-coupled molecular dynamics simulation free from experimental knowledge on binding sites: Application to RNA-ligand binding free-energy landscape [0.03%]
一种无需实验信息的基于结合位点的虚拟系统偶联分子动力学模拟及其在RNA-配体结合自由能景观中的应用
Junichi Higo,Kota Kasahara,Shun Sakuraba et al.
Junichi Higo et al.
Ligand-receptor docking simulation is difficult when the biomolecules have high intrinsic flexibility. If some knowledge on the ligand-receptor complex structure or inter-molecular contact sites are presented in advance, the difficulty of d...
A low-cost electric micromanipulator and its application to single-cell electroporation [0.03%]
低成本的电动微操作器及其在单细胞电转染中的应用
Kazuma Shimizu,Norihiko Nishimura,Manato Oku et al.
Kazuma Shimizu et al.
Micromanipulation techniques are essential in studies of cell function, both for single cells and for cell collectives. Various types of micromanipulators are now commercially available. Hydraulic micromanipulators have the advantage of ana...