Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae) [0.03%]
巧马兜铃微卫星标记的开发及其在银马兜铃中的可转移性（豆科）
M Cristina López-Roberts,Luciano Paganucci de Queiroz,Cássio van den Berg
M Cristina López-Roberts
Premise of the study: This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. •...
A clearing protocol for whole tissues: An example using haustoria of Orobanchaceae [0.03%]
一种用于整个组织的清洗方案——以列当科植物菌丝体为例
Jeffery J Morawetz
Jeffery J Morawetz
Premise of the study: Due to lack of success in clearing whole tissues using only classical clearing techniques (e.g., Herr's 4½ solution, KOH, NaOH, lactic acid saturated with chloral hydrate), and because tissue degrad...
Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae) [0.03%]
豌豆（蚕豆族）的37个新型EST-SSR标记的开发与特征分析
Xiaofeng Zhuang,Kevin E McPhee,Tristan E Coram et al.
Xiaofeng Zhuang et al.
Premise of the study: Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea l...
Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive [0.03%]
利用海藻糖添加剂改进顽拗性植物材料DNA的PCR扩增性能
Tharangamala Samarakoon,Shiao Y Wang,Mac H Alford
Tharangamala Samarakoon
Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate ...
Isolation and characterization of 27 microsatellite markers for the endemic species Diplarche multiflora (Ericaceae) [0.03%]
从旋花乌饭树（杜鹃花科）中分离并表征了27个微卫星标记
Lin Zhang,Zhi-Rong Zhang,Lian-Ming Gao
Lin Zhang
Premise of the study: Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. • ...
Development and characterization of microsatellite loci for smooth cordgrass, Spartina alterniflora (Poaceae) [0.03%]
平滑
Wuxia Guo,Yelin Huang,Ziwen He et al.
Wuxia Guo et al.
Premise of the study: Spartina alterniflora is one of the nine most notoriously invasive plants in China. Microsatellite markers were developed for this species to investigate its invasiveness and genetic diversity. • ...
Isolation and characterization of microsatellite loci for a bioenergy grass, Miscanthus sacchariflorus (Poaceae) [0.03%]
一种能源草Miscanthus sacchariflorus(Poaceae)的微卫星分离和定性研究
Hai-Fei Zhou,Shan-Shan Li,Song Ge
Hai-Fei Zhou
Premise of the study: Microsatellite loci were developed for the biomass C4 grass, Miscanthus sacchariflorus, and proved to be suitable markers for population genetic studies and germplasm management of this species. • ...
Annotation and re-sequencing of genes from de novo transcriptome assembly of Abies alba (Pinaceae) [0.03%]
云杉属欧洲白桦树脱氧核糖核酸测序及注释基因
Anna M Roschanski,Bruno Fady,Birgit Ziegenhagen et al.
Anna M Roschanski et al.
Premise of the study: We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. • ...
SSR-patchwork: An optimized protocol to obtain a rapid and inexpensive SSR library using first-generation sequencing technology [0.03%]
一种优化的协议：利用第一代测序技术快速廉价地获得SSR文库(SSR-拼缝)
Antonietta Di Maio,Olga De Castro
Antonietta Di Maio
Premise of the study: We have optimized a version of a microsatellite loci isolation protocol for first-generation sequencing (FGS) technologies. The protocol is optimized to reduce the cost and number of steps, and it co...
Development of 11 microsatellite loci for an endangered herb, Paraisometrum mileense (Gesneriaceae), in southwest China [0.03%]
在中国西南地区开发珍稀植物伞花参(苦苣苔科_paraisometrum_mileense_的11个微卫星位点
An-Jun Tang,Ren-Chao Zhou,Xue-Jun Ge et al.
An-Jun Tang et al.
Premise of the study: Microsatellite markers were developed to investigate the genetic diversity and population structure of the endangered species Paraisometrum mileense, as well as its evolutionary history. • ...