Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation [0.03%]
基于生态滤镜和Angiosperms353的植物DNA条形码研究精度提升：实地与花粉显微镜验证
Reed Clark Benkendorf,Emily J Woodworth,Paul J CaraDonna et al.
Reed Clark Benkendorf et al.
Premise: Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of un...
Advancing phylogenomics in Amaranthaceae sensu stricto: Development and application of a new nuclear target enrichment bait set [0.03%]
狭义 Amarathaceae 亲缘基因组学研究的推进：新的核基因目标富集引物文库的设计与应用
Tina Kiedaisch,Gudrun Kadereit,Anže Žerdoner Čalasan et al.
Tina Kiedaisch et al.
Premise: Current phylogenies of Amaranthaceae sensu stricto (s.s.) are inadequately sampled and resolved to reflect the entire evolutionary history of the lineage, which is likely complex due to at least three whole-genom...
catalogoUCsBR: A new R package and application for creating comprehensive plant species lists for Brazilian Protected Areas [0.03%]
catalogoUCsBR：一个新R软件包和应用程序，用于创建巴西保护区域的全面植物物种名录
Thuane Bochorny,Pablo H A de Melo,Rafaela C Forzza
Thuane Bochorny
Premise: The increased online publication of data associated with Brazilian botanical collections and biodiversity information systems has significantly improved access to information on plant species occurring in Brazil....
Raspberry Pi-powered temperature monitoring of growth chamber microclimates [0.03%]
基于树莓派的生长室微气候温度监测
Joseph Ballenger,Samuel Kenney,Jared Gordon et al.
Joseph Ballenger et al.
Premise: While controlled environments are desirable for growing and measuring plants, growth chambers and greenhouses typically have microclimates that impact plant growth, development, and stress responses. Furthermore,...
From WGS to gels: Development and testing of PCR primers targeting toxic Digitalis in support of food safety [0.03%]
从WGS到胶体：开发和测试针对有毒Digitalis的PCR引物以支持食品安全发展的毒植物数字草PCR检测方法的发展与验证
Elizabeth Sage Hunter,Sydnee Fo,Robert Literman et al.
Elizabeth Sage Hunter et al.
Premise: This study capitalized on a library of single-nucleotide polymorphisms created via whole genome sequencing (WGS) to develop and test a PCR assay for detecting toxic Digitalis species in food products. Complex foo...
Effectiveness of interval photography cameras for a survey of pollinator communities: Comparison with direct observation [0.03%]
间隔摄影相机进行传粉昆虫调查的效果：与直接观察法的比较
Tomohiro Watazu,Masayoshi K Hiraiwa,Masahito Inoue et al.
Tomohiro Watazu et al.
Premise: Pollinator communities have been surveyed through direct observation, which is labor intensive and difficult for monitoring nocturnal pollinators. Interval photography surveys are increasingly used, although the ...
Astragalean819: An Astragalean clade-specific bait set to resolve phylogenetic relationships in Astragalus [0.03%]
Astragalean819：一种 astragalean 干扰钩特定的诱饵集合，可解决 Astragalus 中的系谱关系
Daniele Buono,Gudrun Kadereit,Diego F Morales-Briones
Daniele Buono
Premise: Astragalus (Fabaceae) is the largest genus of flowering plants, with about 3100 species. Most phylogenies of the genus are based on a few nuclear or plastid loci (with one exception that uses ~100 loci) and usual...
A practical and easy-to-scale protocol for removing chlorophylls from leaf extracts [0.03%]
一种实用且易于扩展的从叶片提取物中去除叶绿素的方法
Alice Fossati,Valeria Cavalloro,Daniela Rossi et al.
Alice Fossati et al.
Premise: Leaf extracts are valuable sources of bioactive compounds. However, co-extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal ofte...
Xinyu Yuan,Nathaniel S S Smith,Gaurav D Moghe
Xinyu Yuan
Plant metabolomes are structurally diverse. One of the most popular techniques for sampling this diversity is liquid chromatography-mass spectrometry (LC-MS), which typically detects thousands of peaks from single organ extracts, many repre...
Anya Lillemor Lindström Battle,Lee James Sweetlove
Anya Lillemor Lindström Battle
Premise: The model liverwort Marchantia polymorpha is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiolog...