Geneious! Simplified genome skimming methods for phylogenetic systematic studies: A case study in Oreocarya (Boraginaceae) [0.03%]
基因组简化在系统发育研究中的应用——基于萝藦狗娃花属(桔梗科)的案例研究
Lee A Ripma,Michael G Simpson,Kristen Hasenstab-Lehman
Lee A Ripma
Premise of the study: As systematists grapple with how to best harness the power of next-generation sequencing (NGS), a deluge of review papers, methods, and analytical tools make choosing the right method difficult. Oreo...
A review of the prevalence, utility, and caveats of using chloroplast simple sequence repeats for studies of plant biology [0.03%]
评价使用叶绿体简单序列重复研究植物生物学的普遍性、实用性和局限性的回顾
Gregory L Wheeler,Hanna E Dorman,Alenda Buchanan et al.
Gregory L Wheeler et al.
Microsatellites occur in all plant genomes and provide useful markers for studies of genetic diversity and structure. Chloroplast microsatellites (cpSSRs) are frequently targeted because they are more easily isolated than nuclear microsatel...
Gene prediction and annotation in Penstemon (Plantaginaceae): A workflow for marker development from extremely low-coverage genome sequencing [0.03%]
花棒属(植物AIN科)基因预测与注释:基于极低覆盖度基因组测序的分子标记开发流程
Paul D Blischak,Aaron J Wenzel,Andrea D Wolfe
Paul D Blischak
Premise of the study: Penstemon (Plantaginaceae) is a large and diverse genus endemic to North America. However, determining the phylogenetic relationships among its 280 species has been difficult due to its recent evolut...
Microsatellite loci in two epiphytic lichens with contrasting dispersal modes: Nephroma laevigatum and N. parile (Nephromataceae) [0.03%]
两种具有不同传播方式的地衣型苔蘚藻(Nephromataceae)中的微卫星基因:Nephroma laevigatum和N. parile
Rocío Belinchón,Christopher J Ellis,Rebecca Yahr
Rocío Belinchón
Premise of the study: Microsatellite markers were characterized for two epiphytic cyanolichens, Nephroma laevigatum and N. parile (Nephromataceae), and will be used to investigate population structure and estimate gene fl...
Isolation and characterization of polymorphic microsatellite loci in Spondias radlkoferi (Anacardiaceae) [0.03%]
从无患子科鲁德克弗石南树中分离并鉴定多态性微卫星座位
Esther Aguilar-Barajas,Victoria L Sork,Arturo González-Zamora et al.
Esther Aguilar-Barajas et al.
Premise of the study: Microsatellite markers were developed for Spondias radlkoferi to assess the impact of primate seed dispersal on the genetic diversity and structure of this important tree species of Anacardiaceae. •...
Development of multiplex microsatellite PCR panels for the seagrass Thalassia hemprichii (Hydrocharitaceae) [0.03%]
用于海洋植物Thalassia hemprichii的多重微卫星PCR面板的研发
Kor-Jent van Dijk,Jane Mellors,Michelle Waycott
Kor-Jent van Dijk
Premise of the study: New microsatellites were developed for the seagrass Thalassia hemprichii (Hydrocharitaceae), a long-lived seagrass species that is found throughout the shallow waters of tropical and subtropical Indo...
Characterization of microsatellite markers for pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing [0.03%]
基于Illumina MiSeq测序的丛萼犁头蕾(Ericaceae)微卫星图谱特征描述
Lisa C Grubisha,Bailey A Nelson,Nicholas J Dowie et al.
Lisa C Grubisha et al.
Premise of the study: Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations. Microsatellit...
Morgan J Randall,Justine Karst,Gregory J Pec et al.
Morgan J Randall et al.
Premise of the study: Roots play a key role in many ecological processes, yet our ability to identify species from bulk root samples is limited. Molecular tools may be used to identify species from root samples, but they ...
Genome and metagenome sequencing: Using the human methyl-binding domain to partition genomic DNA derived from plant tissues [0.03%]
基因组和元基因组测序:使用人类甲基结合域对来自植物组织的基因组DNA进行分区
Erbay Yigit,David I Hernandez,Joshua T Trujillo et al.
Erbay Yigit et al.
Premise of the study: Variation in the distribution of methylated CpG (methyl-CpG) in genomic DNA (gDNA) across the tree of life is biologically interesting and useful in genomic studies. We illustrate the use of human me...
A simple, inexpensive, and field-relevant microcosm tidal simulator for use in marsh macrophyte studies [0.03%]
一种简单、廉价且适用于沼泽大型水生植物研究的潮汐模拟装置
Rachel M MacTavish,Risa A Cohen
Rachel M MacTavish
Premise of the study: A microcosm unit with tidal simulation was developed to address the challenge of maintaining ecologically relevant tidal regimes while performing controlled greenhouse experiments on smooth cordgrass...