From WGS to gels: Development and testing of PCR primers targeting toxic Digitalis in support of food safety [0.03%]
从WGS到胶体：开发和测试针对有毒Digitalis的PCR引物以支持食品安全发展的毒植物数字草PCR检测方法的发展与验证
Elizabeth Sage Hunter,Sydnee Fo,Robert Literman et al.
Elizabeth Sage Hunter et al.
Premise: This study capitalized on a library of single-nucleotide polymorphisms created via whole genome sequencing (WGS) to develop and test a PCR assay for detecting toxic Digitalis species in food products. Complex foo...
Effectiveness of interval photography cameras for a survey of pollinator communities: Comparison with direct observation [0.03%]
间隔摄影相机进行传粉昆虫调查的效果：与直接观察法的比较
Tomohiro Watazu,Masayoshi K Hiraiwa,Masahito Inoue et al.
Tomohiro Watazu et al.
Premise: Pollinator communities have been surveyed through direct observation, which is labor intensive and difficult for monitoring nocturnal pollinators. Interval photography surveys are increasingly used, although the ...
Astragalean819: An Astragalean clade-specific bait set to resolve phylogenetic relationships in Astragalus [0.03%]
Astragalean819：一种 astragalean 干扰钩特定的诱饵集合，可解决 Astragalus 中的系谱关系
Daniele Buono,Gudrun Kadereit,Diego F Morales-Briones
Daniele Buono
Premise: Astragalus (Fabaceae) is the largest genus of flowering plants, with about 3100 species. Most phylogenies of the genus are based on a few nuclear or plastid loci (with one exception that uses ~100 loci) and usual...
A practical and easy-to-scale protocol for removing chlorophylls from leaf extracts [0.03%]
一种实用且易于扩展的从叶片提取物中去除叶绿素的方法
Alice Fossati,Valeria Cavalloro,Daniela Rossi et al.
Alice Fossati et al.
Premise: Leaf extracts are valuable sources of bioactive compounds. However, co-extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal ofte...
Xinyu Yuan,Nathaniel S S Smith,Gaurav D Moghe
Xinyu Yuan
Plant metabolomes are structurally diverse. One of the most popular techniques for sampling this diversity is liquid chromatography-mass spectrometry (LC-MS), which typically detects thousands of peaks from single organ extracts, many repre...
Anya Lillemor Lindström Battle,Lee James Sweetlove
Anya Lillemor Lindström Battle
Premise: The model liverwort Marchantia polymorpha is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiolog...
The Computer-Assisted Sequence Annotation (CASA) workflow for enzyme discovery [0.03%]
计算机辅助序列标注(CASA)_workflow在酶的发现中的应用
Gemma R Takahashi,Franchesca M Cumpio,Carter T Butts et al.
Gemma R Takahashi et al.
Premise: With the advent of inexpensive nucleic acid sequencing and automated annotation at the level of basic functionality, the central problem of enzyme discovery is no longer finding active sequences, it is determinin...
A new spin on chemotaxonomy: Using non-proteogenic amino acids as a test case [0.03%]
化学分类学的新视角：以非蛋白氨基酸为例进行研究
Makenzie Gibson,William Thives Santos,Alan R Oyler et al.
Makenzie Gibson et al.
Premise: Specialized metabolites serve various roles for plants and humans. Unlike core metabolites, specialized metabolites are restricted to certain plant lineages; thus, in addition to their ecological functions, speci...
Advancing plant metabolic research by using large language models to expand databases and extract labeled data [0.03%]
利用大型语言模型扩充数据库和提取标注数据以推进植物代谢研究
Rachel Knapp,Braidon Johnson,Lucas Busta
Rachel Knapp
Premise: Recently, plant science has seen transformative advances in scalable data collection for sequence and chemical data. These large datasets, combined with machine learning, have demonstrated that conducting plant m...
infinitylists: A Shiny application and R package for rapid generation of place-based species checklists [0.03%]
基于地点的物种清单的快速生成方法：一个Shiny应用和R包InfinityLists
Thomas Mesaglio,Fonti Kar,Hervé Sauquet et al.
Thomas Mesaglio et al.
Premise: Biodiversity researchers often need to answer the question: "Which species of taxon X have been documented in (or near) spatial polygon Y?" Online databases with billions of occurrence records, including vouchere...