Harnessing aquatic plant growth forms to apply European nutrient-enrichment bioindicators to Canadian waters [0.03%]
借鉴欧洲富营养化生物指示植物的生长型，建立加拿大水域的生物指标体系
Christopher D Tyrrell,Patricia A Chambers,Joseph M Culp
Christopher D Tyrrell
Premise: Aquatic macrophyte species abundance and nutrient affinity are used in metrics to assess the trophic condition of lakes and rivers. The development of these indices is often regional, with inter-regional comparis...
Using marsh organs to test seed recruitment in tidal freshwater marshes [0.03%]
利用泥炭器官测试潮汐淡水沼泽的种子招募状况
Stefanie L Lane
Stefanie L Lane
Premise: Seed recruitment niches along estuarine elevation gradients are seldom experimentally field-tested under tidal regimes of the Pacific Northwest of North America. Addressing this knowledge gap is important to bett...
Protocol to control the invasive alga Avrainvillea lacerata in a shallow Hawaiian reef flat [0.03%]
控制夏威夷浅海礁平台上入侵藻类Avrainvillea lacerata的协议
Scott E Van De Verg,Celia M Smith
Scott E Van De Verg
Premise: A novel control technique was developed to mitigate an invasive siphonous green alga, Avrainvillea lacerata (Dichotomosiphonaceae), within a shallow degraded reef flat in O'ahu, Hawai'i. ...
Stable isotope mixing models demonstrate the role of an invasive plant in wetland songbird food webs [0.03%]
稳定同位素混合模型展示了入侵植物在湿地鸣鸟食物网中的作用
Rachel D Wigginton,Chloe Van Grootheest,Hildie Spautz et al.
Rachel D Wigginton et al.
Premise: Invasive plants in wetlands are often ecosystem engineers, mediating changes in ecosystem functions like trophic support. We documented the impacts of Lepidium latifolium, an invasive plant, on the food web of om...
Long-read genotyping with SLANG (Simple Long-read loci Assembly of Nanopore data for Genotyping) [0.03%]
使用SLANG（Nanopore数据基因分型的简单长读取定位组装）进行长读取基因分型
Marco Dorfner,Tankred Ott,Philipp Ott et al.
Marco Dorfner et al.
Premise: Most phylogenomic library preparation methods and bioinformatic analysis tools in restriction site-associated DNA sequencing (RADseq)/genotyping-by-sequencing (GBS) studies are designed for use with Illumina data...
Ancient DNA extraction methods for herbarium specimens: When is it worth the effort? [0.03%]
标本馆样品古DNA提取方法：何时值得付出努力？
Pia Marinček,Natascha D Wagner,Salvatore Tomasello
Pia Marinček
Premise: Herbaria harbor a tremendous number of plant specimens that are rarely used for molecular systematic studies, largely due to the difficulty in extracting sufficient amounts of high-quality DNA from the preserved ...
Easy as piadcs: A low-cost, ultra-high-resolution data acquisition system using a Raspberry Pi [0.03%]
基于树莓派的低成本超高分辨率数据采集系统
Anna Knapp,Arnold J Bloom
Anna Knapp
Premise: High-precision data acquisition (DAQ) is essential for developing new methods in the plant sciences. Commercial high-resolution DAQ systems are cost prohibitive, whereas the less expensive systems that are curren...
Development of a petal protoplast transfection system for Sinningia speciosa [0.03%]
一种促进碧冬瓜属植物转染的花瓣原生质体系统发育方法
Zhao-Jun Pan,Yu-Ling Hung,Tsun-Ying Chen et al.
Zhao-Jun Pan et al.
Premise: Transient gene expression systems are powerful tools for studying gene interactions in plant species without available or stable genetic transformation protocols. We optimized a petal protoplast transformation pr...
PhyloHerb: A high-throughput phylogenomic pipeline for processing genome skimming data [0.03%]
叶绿体系统基因组数据的高通量分析流程PhyloHerb
Liming Cai,Hongrui Zhang,Charles C Davis
Liming Cai
Premise: The application of high-throughput sequencing, especially to herbarium specimens, is rapidly accelerating biodiversity research. Low-coverage sequencing of total genomic DNA (genome skimming) is particularly prom...
Genotypic confirmation of a biased phenotypic sex ratio in a dryland moss using restriction fragment length polymorphisms [0.03%]
利用限制性片段长度多态性确认干旱地区苔类植物基因型偏倚的性别比例
Jenna T B Ekwealor,Simone D Benjamin,Jordan Z Jomsky et al.
Jenna T B Ekwealor et al.
Premise: In dioicous mosses, sex is determined by a single U (female, ♀) or V (male, ♂) chromosome. Although a 1 : 1 sex ratio is expected following meiosis, phenotypic sex ratios based on the production of gametangia a...