InDelGT: An integrated pipeline for extracting indel genotypes for genetic mapping in a hybrid population using next-generation sequencing data [0.03%]
InDelGT：利用下一代测序数据在杂交种群中提取indel基因类型进行遗传定位的集成管道
Zhiliang Pan,Jinpeng Zhang,Shengjun Bai et al.
Zhiliang Pan et al.
Premise: Although several software packages are available for genotyping insertion/deletion (indel) polymorphisms in genomes using next-generation sequencing data, simultaneously calling indel genotypes across many indivi...
Whole-genomic DNA amplifications from individually isolated sweet sorghum microspores [0.03%]
单个甜高粱小孢子全基因组DNA扩增
Aron M Felts,Bharat Pokharel,Dilovan Yahya et al.
Aron M Felts et al.
Premise: Sorghum is a multi-use crop, the efficient breeding of which requires the development of new genetic tools. One such tool could be the genetic assessment of free microspores, which are released just after the tet...
Harnessing the power of botanical gardens: Evaluating the costs and resources needed for exceptional plant conservation [0.03%]
充分利用植物园的力量：评估卓越植物保护所需的成本和资源
Megan Philpott,Valerie C Pence,Burgund Bassüner et al.
Megan Philpott et al.
Premise: The effective ex situ conservation of exceptional plants, whether in living collections or cryo-collections, requires more resources than the conservation of other species. Because of their expertise with rare pl...
Seed dormancy and germination of the endangered exceptional Hawaiian lobelioid Brighamia rockii [0.03%]
濒危植物夏威夷 Lobelioid Brighamia rockii 的种子休眠和萌发
Dustin Wolkis,Carol C Baskin,Jerry M Baskin et al.
Dustin Wolkis et al.
Premise: The Campanulaceae (Lobelioideae) is the Hawaiian plant family with the most endangered and extinct species. Although seeds of Hawaiian lobelioids are desiccation tolerant, the species are exceptional (i.e., they ...
Can alpine plant species "bank" on conservation?: Using artificial aging to understand seed longevity [0.03%]
高山植物能否“储备”种子以利于保护？利用人工老化理解种子的寿命上限
Alexandra E Seglias
Alexandra E Seglias
Premise: To conserve native plants, many institutions are turning toward ex-situ conservation methods, such as storage in seed banks; however, not all seeds are able to survive in seed bank conditions, or may not in the l...
Improving culture initiation of mature oak shoots through use of silver thiosulfate [0.03%]
用银硫代硫酸盐处理促进橡树木质部成熟插穗的愈伤组织诱导
Max Winkeljohn,Valerie C Pence,Theresa M Culley
Max Winkeljohn
Premise: Of the approximately 430 species of oaks (Quercus spp.) that have been assessed, 31% are threatened with extinction and in need of safeguarding. However, oak seeds cannot be seed banked, and thus rely on alternat...
Asymbiotic in vitro seed germination, in vitro seedling development, and ex vitro acclimatization of Spiranthes [0.03%]
独脚金内脂的非共生离体萌芽、离体幼苗培养及试管外驯化培育
Peter J Zale,Ashley Clayton,John Nix et al.
Peter J Zale et al.
Premise: Reproducible seed propagation and production protocols were developed for Spiranthes and related taxa to facilitate ex situ conservation practices. ...
Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation [0.03%]
降低内生菌在植物顶芽 cryopreservation 中的不良影响
Gayle M Volk,Remi Bonnart,Annie Carolina Araújo de Oliveira et al.
Gayle M Volk et al.
Plant cryopreservation technologies are used within gene banks for the long-term preservation of vegetatively propagated collections. Surface-sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro a...
The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques [0.03%]
从种子培育兰花以进行植物保护的挑战：无菌培养技术评估
Devani Jolman,Martín I Batalla,Alexis Hungerford et al.
Devani Jolman et al.
Lewis Knudson first successfully germinated orchid seeds asymbiotically on artificial medium in 1922. While many orchid species have since been grown asymbiotically, the tremendous variation in how species respond to artificial medium and g...
Supporting long-term sustainability of ex situ collections using a pedigree-based population management approach [0.03%]
基于谱系的种群管理方法在支持离体收藏长期可持续性方面的作用
Jeremy A Foster,Seana K Walsh,Kayri Havens et al.
Jeremy A Foster et al.
Premise: Living collections maintained for generations are at risk of diversity loss, inbreeding, and adaptation to cultivation. To address these concerns, the zoo community uses pedigrees to track individuals and impleme...