Modified CTAB protocols for high-molecular-weight DNA extractions from ferns [0.03%]
改良CTAB法高效提取蕨类植物高分子量基因组DNA
Pei-Jun Xie,Ya-Ting Ke,Li-Yaung Kuo
Pei-Jun Xie
Premise: Efficient protocols for extracting high-molecular-weight (HMW) DNA from ferns facilitate the long-read sequencing of their large and complex genomes. Here, we perform two cetyltrimethylammonium bromide (CTAB)-bas...
Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues [0.03%]
改良CTAB法提取硅胶干燥叶片和模式标本馆藏叶片基因组DNA抽提中裂解步骤的优化
S James Carey,L Ellie Becklund,Paige P Fabre et al.
S James Carey et al.
Premise: The use of cetyltrimethylammonium bromide (CTAB) is an effective and inexpensive method of extracting DNA from plants. The CTAB protocol is frequently modified to optimize DNA extractions, but experimental approa...
What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol [0.03%]
“改良”的CTAB协议是什么？对CTAB基因组DNA提取方法的改进进行表征
John J Schenk,L Ellie Becklund,S James Carey et al.
John J Schenk et al.
Cetyltrimethylammonium bromide (CTAB)-based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization. Research articles often c...
High-molecular-weight DNA extraction for long-read sequencing of plant genomes: An optimization of standard methods [0.03%]
植物基因组长读段测序的高分子DNA提取法：标准方法的优化
Myoungbo Kang,Andre Chanderbali,Seungyeon Lee et al.
Myoungbo Kang et al.
Premise: Developing an effective and easy-to-use high-molecular-weight (HMW) DNA extraction method is essential for genomic research, especially in the era of third-generation sequencing. To efficiently use technologies c...
A comparison of freezer-stored DNA and herbarium tissue samples for chloroplast assembly and genome skimming [0.03%]
比较储存在冰箱中的DNA和标本室中的植物样品用于叶绿体基因组组装及基因组轻描淡写的效果
Edward V McAssey,Cassidy Downs,Mitsuko Yorkston et al.
Edward V McAssey et al.
Premise: The use of DNA from herbarium specimens is an increasingly important source for evolutionary studies in plant biology, particularly in cases where species are rare or difficult to obtain. Here we compare the util...
DNA assays for genetic discrimination of three Phragmites australis subspecies in the United States [0.03%]
美国三种Phragmites australis亚种的基因鉴别DNA测定方法
Denise L Lindsay,Xin Guan,Nathan E Harms et al.
Denise L Lindsay et al.
Premise: To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real-time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, a...
Multiple Leaf Sample Extraction System (MuLES): A tool to improve automated morphometric leaf studies [0.03%]
多重叶样本提取系统（MuLES）：一种提高自动化形态学叶片研究的工具
Christian S Bowman,Ryan Traband,Xuesong Wang et al.
Christian S Bowman et al.
Premise: The measurement of leaf morphometric parameters from digital images can be time-consuming or restrictive when using digital image analysis softwares. The Multiple Leaf Sample Extraction System (MuLES) is a new to...
Does sorting by color using visible and high-energy violet light improve classification of taxa in honey bee pollen pellets? [0.03%]
可见光和高能紫光源下按颜色分拣蜂蜜花粉团是否能改善其分类效果？
Charlie P Bailey,Carolyn A Sonter,Jeremy L Jones et al.
Charlie P Bailey et al.
Premise: Pollen collected by honey bees from different plant species often differs in color, and this has been used as a basis for plant identification. The objective of this study was to develop a new, low-cost protocol ...
Infrared spectroscopy for ploidy estimation: An example in two species of Veronica using fresh and herbarium specimens [0.03%]
基于红外光谱的倍性水平估计——以紫草属两种植物的模式和标本为例
Daniele Buono,Dirk C Albach
Daniele Buono
Premise: Polyploidy has become a central factor in plant evolutionary biological research in recent decades. Methods such as flow cytometry have revealed the widespread occurrence of polyploidy; however, its inference rel...
Acclimation and hardening of a slow-growing woody species emblematic to western North America from in vitro plantlets [0.03%]
西方北美特有的缓慢生长的木质部植物离体培养植株的驯化和硬化
Peggy Martinez,Marcelo Serpe,Rachael Barron et al.
Peggy Martinez et al.
Premise: Determining the tolerance of plant populations to climate change requires the development of biotechnological protocols producing genetically identical individuals used for genotype-by-environment experiments. Su...