Application of multiplex PCR-based nanopore sequencing for rapid detection and surveillance of antimicrobial-resistant Mycoplasma bovis in Japanese dairy cattle [0.03%]
基于纳米孔测序的多重PCR在快速检测和监测日本乳用奶牛中耐药性支原体中的应用
Masaru Usui,Masashi Ebisawa,Shingo Okamura et al.
Masaru Usui et al.
Mycoplasma bovis causes mastitis and respiratory disease, leading to economic losses in the dairy industry. Conventional culture and antimicrobial susceptibility testing require approximately 2 weeks, promoting empirical antimicrobial use a...
Genomic and phenotypic characterization of non-O157 Shiga toxin-producing Escherichia coli from Bangladeshi cattle reveals diverse virulence and resistance profiles [0.03%]
来自孟加拉国牛的非O157型志贺毒素产生大肠杆菌的基因组和表型特征揭示了不同的毒力和抗性谱系
Tahrima Saiha Huq,Fahad Khan,Aura Rahman et al.
Tahrima Saiha Huq et al.
Shiga toxin-producing Escherichia coli (STEC) are important zoonotic pathogens that cause diarrheal diseases and hemolytic uremic syndrome in humans, but data from Bangladesh remain limited. We collected 352 rectal swabs and 2 retail meat s...
The two-component regulator CvsR has a small core regulon in planta and modulates Pseudomonas syringae global gene expression with some overlap to the pattern-triggered immunity stimulon response [0.03%]
二组分调节因子CvsR在植物中的核心作用子很少，但能调节丁香假单胞菌的全局基因表达，并与病原小体激活的免疫反应存在部分重叠区域
Hsiao-Chun Chen,Carter J Newton,Li Yang et al.
Hsiao-Chun Chen et al.
Pattern-triggered immunity (PTI) provides broad-spectrum protection in plants by activating defense responses upon perception of conserved microbial signatures such as bacterial flagellin. In vitro transcriptome profiling revealed that the ...
Muc16 contributes to protection against invasive pneumococcal infection originating from nasal colonization [0.03%]
Muc16有助于预防鼻部定植肺炎球菌的侵袭性感染
Daichi Murakami,Tatsuya Shiga,Hideki Sakatani et al.
Daichi Murakami et al.
Streptococcus pneumoniae asymptomatically colonizes upper respiratory mucosa and invades into deeper tissue through mucosal membrane. Invasive pneumococcal disease has a significantly high rate of mortality, making its control an urgent con...
Tail-specific protease (Tsp)-mediated envelope remodeling and beta-lactam tolerance in Escherichia coli [0.03%]
大肠杆菌中的尾特异性蛋白酶（Tsp）介导的包膜重塑和β-内酰胺耐受性
Jenet Narzary,Sayed Golam Mohiuddin,Aslan Massahi et al.
Jenet Narzary et al.
Bacterial persisters are transiently drug-tolerant cells that survive antibiotic treatment without genetic resistance. While certain cytosolic proteases are known to regulate persistence through toxin-antitoxin and stress pathways, the role...
Comparative evaluation of EUCAST RAST and QuickMIC for rapid antimicrobial susceptibility testing of carbapenem-resistant organisms directly from positive blood cultures [0.03%]
欧盟委员会RAST和QuickMIC的比较评估：直接从阳性血液培养中快速检测碳青霉烯类耐药生物的抗菌药物敏感性试验
Nicole Degel-Brossmann,Tom Kimkes,Lucas Reibenspies et al.
Nicole Degel-Brossmann et al.
Rapid availability of phenotypic susceptibility results is crucial for the timely detection of multidrug-resistant Gram-negative organisms. Several novel methods have been introduced that enable direct antimicrobial susceptibility testing (...
Systematic discovery of novel phosphoinositide-binding effectors in Legionella reveals conserved ɑ-helical folds [0.03%]
系统发现Legionella中新型磷脂酰肌醇结合效应物揭示了保守的α-螺旋折叠结构
Abigail E Bolt,Abby E Richardson,Colleen M Pike et al.
Abigail E Bolt et al.
Intracellular bacterial pathogens deploy effector proteins to hijack host membranes by targeting specific lipids. Phosphoinositides are key eukaryotic signaling molecules that govern membrane identity and dynamics, making them powerful leve...
ZILA-SRM: a probabilistic framework with zero-inflated latent models for robust strain reconstruction from metagenomes [0.03%]
具有零膨胀潜在模型的稳健菌株重建的概率框架ZILA-SRM
Saidi Wang,Mintong Chen,Di Jiao
Saidi Wang
Resolving bacterial strain diversity from shotgun metagenomic data is fundamental to understanding intra-host evolution, transmission dynamics, and phenotypic heterogeneity. However, current probabilistic approaches face a severe "identifia...
Emergence and persistence of ESBL- and carbapenemase-producing Klebsiella pneumoniae-related species in Barcelona wastewater treatment plants [0.03%]
产ESBL和碳青霉烯酶肺炎克雷伯菌种属在巴塞罗那污水处理厂中的出现与存在状况研究
Victoria Ballén,Karol Delgado,Anna Pinar-Méndez et al.
Victoria Ballén et al.
The World Health Organization classifies extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Klebsiella pneumoniae as critical-priority pathogens due to their high incidence, mortality, transmissibility, rapid resistance acq...
Impact of early vs delayed initiation of dual antimicrobial salvage therapy on clinical outcomes in MRSA bacteremia [0.03%]
万古霉素联合利奈唑胺早期或延迟起始序贯治疗MRSA菌血症的临床结局
Victoria Sanderford,Nikko Rowe A Tabliago,Sarah Alnami et al.
Victoria Sanderford et al.
Our retrospective observational cohort study investigated the effect of the timing of escalation to dual salvage therapy utilizing ceftaroline with vancomycin or daptomycin for the treatment of persistent methicillin-resistant Staphylococcu...