Mouse Model of Burn Wound and Infection: Thermal (Hot Air) Lesion-Induced Immunosuppression [0.03%]
烧伤与感染小鼠模型：热（热空气）损伤诱导的免疫抑制现象
Henrik Calum,Niels Høiby,Claus Moser
Henrik Calum
The immunosuppression induced by thermal injury renders the burned victim susceptible to infection. A mouse model was developed to examine the immunosuppression, which was possible to induce even at a minor thermal insult of 6% total body s...
A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse [0.03%]
一种快速、简单和可定制的八色流式细胞法分析小鼠支气管肺泡灌洗液细胞组成的方法
François Daubeuf,Julien Becker,Juan Antonio Aguilar-Pimentel et al.
François Daubeuf et al.
The cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is commonly determined by cytocentrifuging leukocytes on slides, then staining, identifying, and counting them as eosinophils, n...
Daria Kolmogorova,Emma Murray,Nafissa Ismail
Daria Kolmogorova
Sickness behavior monitoring, a technique for examining the development of sickness symptomatology following infection, is necessary in experiments studying neurochemical and physiological changes associated with pathogen-induced immune act...
Zhiyong Zhang,Osama Abdel-Razek,Guirong Wang
Zhiyong Zhang
Creation of an appropriate animal model that accurately reflects the disease and host immune response to bacterial infection in humans is a major challenge in ocular-surface infection research. For decades, mice have been the ideal small an...
Non-Typeable Haemophilus influenzae Infection of the Junbo Mouse [0.03%]
新型流感嗜血杆菌对俊博鼠的感染效应研究进展及今后的工作方向
Michael T Cheeseman,Derek W Hood
Michael T Cheeseman
Acute otitis media, inflammation of the middle ear bulla, is the most common bacterial infection in children. For one of the principal otopathogens, non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate host-micr...
Exploration of Inflammatory Bowel Disease in Mice: Chemically Induced Murine Models of Inflammatory Bowel Disease (IBD) [0.03%]
小鼠化学诱导性肠炎模型在炎症性肠病(IBD)研究中的应用
Raffaella Maria Gadaleta,Oihane Garcia-Irigoyen,Antonio Moschetta
Raffaella Maria Gadaleta
Inflammatory bowel disease (IBD) is a chronic multifactorial inflammatory disorder characterized by periods of activation and remission of intestinal inflammation, with potentially severe complications, that can lead to mortality. Experimen...
Pedro M Quiros,Aashima Goyal,Pooja Jha et al.
Pedro M Quiros et al.
Mitochondrial DNA (mtDNA) lacks the protection provided by the nucleosomes in the nuclear DNA and does not have a DNA repair mechanism, making it highly susceptible to damage, which can lead to mtDNA depletion. mtDNA depletion compromises t...
Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations [0.03%]
Cre-LoxP 重组：基本原则与实验注意事项
Micheal A McLellan,Nadia A Rosenthal,Alexander R Pinto
Micheal A McLellan
The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mo...
Methodological Considerations for Optimizing and Validating Behavioral Assays [0.03%]
行为测定法的优化和验证的方法学考虑因素
Stacey J Sukoff Rizzo,Jill L Silverman
Stacey J Sukoff Rizzo
Preclinical animal models are indispensable tools for translational research for which behavioral characterization and phenotyping are essential to testing hypotheses and for evaluating the potential of novel therapeutic agents to treat dis...
Measurement of Behavioral Taste Responses in Mice: Two-Bottle Preference, Lickometer, and Conditioned Taste-Aversion Tests [0.03%]
小鼠行为味觉反应的测定：两瓶偏好、舔速仪和条件性味道厌恶试验
Dany Gaillard,Jennifer M Stratford
Dany Gaillard
The natural like and dislike of foods based on taste is one of the most easily observed behaviors in animals. Animals eat palatable foods and reject aversive foods, which makes measurement of taste perception possible using various behavior...