Quantification of Neutrophil Extracellular Traps Isolated From Mouse Tissues [0.03%]
从小鼠组织中量化 neutrophil 外分泌陷阱
Rebecca L Buckland,Alicia S Wilson,Anne Brüstle
Rebecca L Buckland
One of the most intriguing functions of neutrophils is the production of neutrophil extracellular traps (NETs), which are formed when neutrophils decondense their internal DNA and extrude it along with cytotoxic proteins in a web-like struc...
The Kinetics of Intestinal Permeability in a Mouse Model of Traumatic Brain Injury [0.03%]
创伤性脑损伤小鼠模型中肠通透性的动力学研究
Jessica L Weaver
Jessica L Weaver
Traumatic brain injury (TBI) is a leading cause of morbidity and mortality among trauma patients. Increased intestinal permeability plays an important role in the inflammatory process that accompanies TBI, and therapies that prevent this pe...
An Ex Vivo Acid Injury and Repair (AIR) Model Using Precision-Cut Lung Slices to Understand Lung Injury and Repair [0.03%]
一种使用精确切割的肺片进行体外酸损伤和修复(AIR)模型以理解肺损伤和修复的方法
Sally Yunsun Kim,Róisín Mongey,Mark Griffiths et al.
Sally Yunsun Kim et al.
Recent advances in cell culture models like air-liquid interface culture and ex vivo models such as organoids have advanced studies of lung biology; however, gaps exist between these models and tools that represent the complexity of the thr...
Tackling the Mouse-on-Mouse Problem in Cochlear Immunofluorescence: A Simple Double-Blocking Protocol for Immunofluorescent Labeling of Murine Cochlear Sections with Primary Mouse Antibodies [0.03%]
解决耳蜗免疫荧光中的鼠源抗体交叉反应问题：使用一抗鼠源抗体对小鼠耳蜗切片进行免疫荧光标记的简单双重封闭方案
Mohamed Bassiouni,Katharina Stölzel,Alina Smorodchenko et al.
Mohamed Bassiouni et al.
The mouse is the most widely used animal model in hearing research. Immunohistochemistry and immunofluorescent staining of murine cochlear sections have, thus, remained a backbone of inner ear research. Since many primary antibodies are rai...
Quantitative Proteomic Profiling of Murine Ocular Tissue and the Extracellular Environment [0.03%]
小鼠眼部组织及细胞外环境的定量蛋白质谱分析
Jason Yeung,Jeffrey Lamb,Jonathan R Krieger et al.
Jason Yeung et al.
Mass spectrometry-based proteomics provides a robust and reliable method for detecting and quantifying changes in protein abundance among samples, including cells, tissues, organs, and supernatants. Physical damage or inflammation can compr...
Assessment of Problem-Solving Skills and Inhibitory Control in Mice Using Water Escape Detour Test [0.03%]
使用水域逃逸绕路测试对小鼠解决问题的能力和抑制控制进行评估
Grzegorz R Juszczak,Anna Bobrowska
Grzegorz R Juszczak
Despite the importance of emotional intelligence, its biological mechanism is still not well understood. For this reason, we have developed a rodent detour task which requires an animal to reach a highly desired object placed directly behin...
Simultaneous Assessment of Circadian Rhythms and Sleep in Mice Using Passive Infrared Sensors: A User's Guide [0.03%]
使用被动红外传感器同时评估小鼠的生物钟节律和睡眠：用户指南
Laurence A Brown,Gareth T Banks,Neil Horner et al.
Laurence A Brown et al.
The 24-hr cycle of activity and sleep provides perhaps the most familiar example of circadian rhythms. In mammals, circadian activity rhythms are generated by a master biological clock located in the hypothalamic suprachiasmatic nuclei (SCN...
Phenotyping in Mice Using Continuous Home Cage Monitoring and Ultrasonic Vocalization Recordings [0.03%]
使用连续笼养监测和超声波发声记录进行小鼠表型分析
Liane Hobson,Rasneer S Bains,Simon Greenaway et al.
Liane Hobson et al.
Over the last century, the study of mouse behavior has uncovered insights into brain molecular mechanisms while revealing potential causes of many neurological disorders. To this end, researchers have widely exploited the use of mutant stra...
Randall J Roper,Charles R Goodlett,María Martínez de Lagrán et al.
Randall J Roper et al.
Down syndrome (DS) is the most frequent genetic cause of intellectual disability, characterized by alterations in different behavioral symptom domains: neurodevelopment, motor behavior, and cognition. As mouse models have the potential to g...
Annemiek Beverdam
Annemiek Beverdam
In situ hybridization is a powerful technique that allows the visualization of specific RNA species in biological samples in exquisite detail. It has been particularly well explored in the field of developmental genetics. The spatial and te...