Pierre L Roubertoux,Adeline Ghata,Michèle Carlier
Pierre L Roubertoux
The immaturity at birth and the slowness of ontogenic processes in mice provide the opportunity to measure rates of development. We describe here 18 measures covering the sensorial and motor onset from birth to weaning. The measures are non...
Junzo Kinoshita,Neal S Peachey
Junzo Kinoshita
Overall retinal function can be monitored by recording the light-evoked response of the eye at the corneal surface. The major components of the electroretinogram (ERG) provide important information regarding the functional status of many re...
Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease [0.03%]
利用Cas9导向性核酸酶在小鼠中工程化点突变和表位标签等位基因
Marina Gertsenstein,Lauryl M J Nutter
Marina Gertsenstein
Mice carrying patient-associated point mutations are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alle...
Analysis of Neuropsychiatric Disease-Related Functional Neuroanatomical Markers in Mice [0.03%]
小鼠神经精神疾病相关功能神经解剖标志的分析研究
Lillian Garrett,Marie-Claire Ung,Tamara Heermann et al.
Lillian Garrett et al.
A better alignment of preclinical and clinical neurobiological measures could help improve neuropsychiatric disease therapeutic development. This unit describes a compendium of hypothesis-driven neuroanatomical phenotyping strategies to be ...
Devra D Huey,Stefan Niewiesk
Devra D Huey
The development of humanized mice has become a prominent tool for translational animal studies of human diseases. Here we show how immune deficient mice can be "humanized" by injections of human umbilical cord stem cells. The engraftment of...
A Method for Parasagittal Sectioning for Neuroanatomical Quantification of Brain Structures in the Adult Mouse [0.03%]
成年小鼠脑结构神经解剖定量的 parasagittal 切割方法
Stephan C Collins,Christel Wagner,Léo Gagliardi et al.
Stephan C Collins et al.
In this article, we present a standardized protocol for fast and robust neuroanatomical phenotyping of the adult mouse brain, which complements a previously published article (doi: 10.1002/cpmo.12) in Current Protocols in Mouse Biology. It ...
Assessment of Topographic Memory in Mice in a Complex Environment Using the Hamlet Test [0.03%]
利用汉默尔特测试评估小鼠在复杂环境中的地形记忆能力
Lucie Crouzier,Tangui Maurice
Lucie Crouzier
Here we provide instructions to measure topographic memory in mice using the Hamlet test, a complex environment. The apparatus mimics a small hamlet with a central agora and five houses, which are functionalized since mice can drink, eat pe...
Brian J Nieman,Matthijs C van Eede,Shoshana Spring et al.
Brian J Nieman et al.
This article describes a detailed set of protocols for mouse brain imaging using MRI. We focus primarily on measuring changes in neuroanatomy, and provide both instructions for mouse preparation and details on image acquisition, image proce...
Pilib Ó Broin,Michael V Beckert,Tomohisa Takahashi et al.
Pilib Ó Broin et al.
Neonatal vocalization is structurally altered in mouse models of autism spectrum disorder (ASD). Our published data showed that pup vocalization, under conditions of maternal separation, contains sequences whose alterations in a genetic mou...
Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice [0.03%]
诱导和鉴定产生慢性及衰老表型的小鼠突变体的方法
Andrew Blease,Thomas Nicol,Sara Falcone et al.
Andrew Blease et al.
Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging ...