Jeremy Sanderson
Jeremy Sanderson
The light (or optical) microscope is the icon of science. The aphorism "seeing is believing" is often quoted in scientific papers involving microscopy. Unlike many scientific instruments, the light microscope will deliver an image however b...
Whole-Mount In Situ Hybridization in Post-Implantation Staged Mouse Embryos [0.03%]
植入后小鼠胚胎整体原位杂交检测技术
Kristen S Barratt,Ruth M Arkell
Kristen S Barratt
Understanding RNA expression in space and time is a key initial step in dissecting gene function. The ability to visualize gene expression in whole-tissue or whole-specimen preparations, called in situ hybridization (ISH), was first develop...
Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments [0.03%]
地高辛标记的RNA探针的制备及原位杂交实验操作方法学研究
Kristen S Barratt,Ruth M Arkell
Kristen S Barratt
Experiments that visualize gene expression in intact tissues or organisms are fundamental to studies of gene function. These experiments, called in situ hybridization, require the production of a riboprobe, which is a labeled antisense RNA ...
Simple Protocol for Generating and Genotyping Genome-Edited Mice With CRISPR-Cas9 Reagents [0.03%]
一种生成和基因分型CRISPR-Cas9试剂制备的基因修饰小鼠的简易方法
Almudena Fernández,Matías Morín,Diego Muñoz-Santos et al.
Almudena Fernández et al.
The simple protocol described in this article aims to provide all required information, as a comprehensive, easy-to-follow step-by-step method, to ensure the generation of the expected genome-edited mice. Here, we provide protocols for the ...
Martin Darvas,Kishore Mukherjee,Amanda Lee et al.
Martin Darvas et al.
Current preclinical cognitive assessments are highly time intensive, with lengthy assessment procedures. In this regard, a single-day assay that focuses just on assessing learning behavior in a time-effective and relatable manner would be o...
Efficient Generation of Large-Fragment Knock-In Mouse Models Using 2-Cell (2C)-Homologous Recombination (HR)-CRISPR [0.03%]
利用2细胞(C2)同源重组(HR)-CRISPR高效构建大型片段敲入小鼠模型
Bin Gu,Eszter Posfai,Marina Gertsenstein et al.
Bin Gu et al.
Generating large-fragment knock-ins, such as reporters, conditional alleles, or humanized alleles, directly in mouse embryos is still a challenging feat. We have developed 2C-HR-CRISPR, a technology that allows highly efficient (10-50%) and...
Designing a QTL Mapping Study for Implementation in the Realized Collaborative Cross Genetic Reference Population [0.03%]
设计用于实现在协作交叉遗传参考群体中的数量性状位点映射研究
Morris Soller,Hanifa J Abu-Toamih Atamni,Ilona Binenbaum et al.
Morris Soller et al.
The Collaborative Cross (CC) mouse resource is a next-generation mouse genetic reference population (GRP) designed for high-resolution mapping of quantitative trait loci (QTL) of large effect affecting complex traits during health and disea...
Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation [0.03%]
用于小鼠基因型鉴定的PCR优化：可靠、快速、低成本且适用于任何类型突变的高通量基因分型协议建议
Sylvie Jacquot,Nathalie Chartoire,Françoise Piguet et al.
Sylvie Jacquot et al.
Genotyping consists of searching for a DNA sequence variation localized at a well-defined locus in the genome. It is an essential step in animal research because it allows the identification of animals that will be bred to generate and main...
Genomic Mutation Identification in Mice Using Illumina Sequencing and Linux-Based Computational Methods [0.03%]
基于 illumina 测序和 linux 信息分析技术的基因组突变小鼠鉴定方法
John A Williams,George Powell,Ann-Marie Mallon et al.
John A Williams et al.
Genetically modified mice are an essential tool for modeling disease-causing mechanisms and discovering gene function. SNP genotyping was traditionally used to associate candidate regions with traits in the mouse, but failed to reveal novel...
High Resolution Imaging of Mouse Embryos and Neonates with X-Ray Micro-Computed Tomography [0.03%]
基于微焦点CT的小鼠胚胎和幼仔高分辨率三维显微影像研究
Chih-Wei Hsu,Sowmya Kalaga,Uchechukwu Akoma et al.
Chih-Wei Hsu et al.
Iodine-contrast micro-computed tomography (microCT) 3D imaging provides a non-destructive and high-throughput platform for studying mouse embryo and neonate development. Here we provide protocols on preparing mouse embryos and neonates betw...