Evaluation of Rett Syndrome Symptom Improvement by Metabolic Modulators in Mecp2-Mutant Mice [0.03%]
代谢调节剂对Mecp2突变小鼠雷特综合症症状改善的评估
Christie M Buchovecky,Misty G Hill,Jennifer M Borkey et al.
Christie M Buchovecky et al.
Mouse models recapitulate many symptoms of Rett Syndrome, an X-linked disorder caused by mutations in methyl-CpG-binding protein 2 (MECP2). The study of Mecp2-null male mice has provided insight into pathogenesis of the disorder-most recent...
Tissue-Specific Regulation of Oncogene Expression Using Cre-Inducible ROSA26 Knock-In Transgenic Mice [0.03%]
使用Cre诱导型ROSA26敲入转基因小鼠进行肿瘤基因表达的组织特异性调节
Brandi L Carofino,Monica J Justice
Brandi L Carofino
Cre-inducible mouse models are often utilized for the spatial and temporal expression of oncogenes. With the wide number of Cre recombinase lines available, inducible transgenesis represents a tractable approach to achieve discrete oncogene...
Martin Leeb,Anthony C F Perry,Anton Wutz
Martin Leeb
Haploid genetics has facilitated new insights into mammalian pathways and disease mechanisms. Most animal cells are diploid, and mammalian haploid cell cultures have remained elusive for a long time. Recent methodological progress has enabl...
High-Resolution Respirometry for Mitochondrial Characterization of Ex Vivo Mouse Tissues [0.03%]
高分辨率呼吸测定法表征离体小鼠组织的线粒体特性
Carles Cantó,Pablo M Garcia-Roves
Carles Cantó
This article describes methodologies to examine mitochondrial respiration in fresh preparations of mouse tissues, including skeletal muscle, heart, liver, white and brown adipose tissue, and brain. Reference values and tips to maximize expe...
Cheryl L Ackert-Bicknell,Laura C Anderson,Susan Sheehan et al.
Cheryl L Ackert-Bicknell et al.
Despite the dramatic increase in human lifespan over the past century, there remains pronounced variability in "health-span," or the period of time in which one is generally healthy and free of disease. Much of the variability in health-spa...
Bryna S M Chow,Terri J Allen
Bryna S M Chow
Diabetic nephropathy (DN) is a term used to describe kidney damage cause by diabetes. With DN as one of the leading causes of end-stage renal disease worldwide, there is a strong need for appropriate animal models to study DN pathogenesis a...
Dinorah Friedmann-Morvinski,Oded Singer
Dinorah Friedmann-Morvinski
Glioblastoma multiforme (GBM) is one of the most common and most malignant of the brain tumors. Gliomas can be classified into four different grades according to their histologic characteristics; the most aggressive of the gliomas is gliobl...
Birgit Rathkolb,Helmut Fuchs,Valérie Gailus-Durner et al.
Birgit Rathkolb et al.
Basic phenotyping of inbred mouse strains and genetically modified mouse models usually includes the determination of blood-based parameters as a diagnostic screen for genotype effects on metabolism and organ function. A broad range of anal...
Birgit Rathkolb,Wolfgang Hans,Cornelia Prehn et al.
Birgit Rathkolb et al.
Besides hematological analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these i...
A Comprehensive Guide to Sleeping Beauty-Based Somatic Transposon Mutagenesis in the Mouse [0.03%]
基于睡眠美人系统的小鼠体细胞转座因子突变的全面指南
Branden Moriarity,David A Largaespada
Branden Moriarity
Recent advances in whole genome analyses made possible by next-generation DNA sequencing, high-density array comparative genome hybridization (aCGH), and other technologies have made it apparent that cancers harbor numerous genomic changes....