RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells [0.03%]
二聚小花椰菜活细菌和哺乳动物细胞RNA成像
Grigory S Filonov,Samie R Jaffrey
Grigory S Filonov
RNA spatial dynamics play a crucial role in cell physiology, and thus the ability to monitor RNA localization in live cells can provide insight into important biological problems. This unit focuses on imaging RNAs using an RNA mimic of GFP....
Selecting, Acquiring, and Using Small Molecule Libraries for High-Throughput Screening [0.03%]
用于高通量筛选的小分子文库的选择、获取及使用方法
Sivaraman Dandapani,Gerard Rosse,Noel Southall et al.
Sivaraman Dandapani et al.
The selection, acquisition and use of high quality small molecule libraries for screening is an essential aspect of drug discovery and chemical biology programs. Screening libraries continue to evolve as researchers gain a greater appreciat...
Justin M Roberts,James E Bradner
Justin M Roberts
Bromodomain-containing proteins have emerged as desirable targets for anti-neoplastic and anti-inflammatory drug discovery. Toward the development of selective inhibitors of the BET family of bromodomains, we optimized bead-based assays to ...
Corking Nitrogen-Doped Carbon Nanotube Cups with Gold Nanoparticles for Biodegradable Drug Delivery Applications [0.03%]
用于生物降解药物传输的镀金氮掺杂碳纳米杯
Seth C Burkert,Alexander Star
Seth C Burkert
Carbon nanomaterials have been proposed as effective drug delivery devices; however their perceived biopersistence and toxicological profile may hinder their applications in medical therapeutics. Nitrogen doping of carbon nanotubes results ...
Hosahalli P Hemantha,Somasekhar Bondalapati,Sumeet K Singh et al.
Hosahalli P Hemantha et al.
Chemical synthesis of complex biomolecules such as proteins is a challenging adventure, yet rewarding in driving various biochemical and biophysical research activities. Over the years, the refinement of peptide synthesis and invention of l...
Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids [0.03%]
分子演化利用非天然氨基酸指导蛋白质合成
Vanessa E Cox,Eric A Gaucher
Vanessa E Cox
Unnatural amino acids have in recent years established their importance in a wide range of fields, from pharmaceuticals to polymer science. Unnatural amino acids can increase the number of chemical groups within proteins and thus expand or ...
Dealing with the Data Deluge: Handling the Multitude Of Chemical Biology Data Sources [0.03%]
应对数据洪流：处理众多的化学生物学数据来源
Rajarshi Guha,Dac-Trung Nguyen,Noel Southall et al.
Rajarshi Guha et al.
Over the last 20 years, there has been an explosion in the amount and type of biological and chemical data that has been made publicly available in a variety of online databases. While this means that vast amounts of information can be foun...
Identifying Direct Protein Targets of Poly-ADP-Ribose Polymerases (PARPs) Using Engineered PARP Variants-Orthogonal Nicotinamide Adenine Dinucleotide (NAD+) Analog Pairs [0.03%]
利用工程改造型PARP变异体-正交烟酰胺腺嘌呤二核苷酸(NAD+)对对聚(腺苷二磷酸核糖)聚合酶(PARP)的直接蛋白靶标进行鉴定
Ian Carter-O&#x;Connell,Michael S Cohen
Ian Carter-O&#x;Connell
Poly-ADP-ribose polymerases (PARPs) comprise a family of 17 distinct enzymes that catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to acceptor sites on protein targets. PARPs have been implicated in a number...
Point by Point: An Introductory Guide to Sample Preparation for Single-Molecule, Super-Resolution Fluorescence Microscopy [0.03%]
单分子超分辨率荧光显微镜样品制备入门指南
Aaron R Halpern,Marco D Howard,Joshua C Vaughan
Aaron R Halpern
Single-molecule, localization-based, super resolution microscopy is able to reveal detailed subcellular structures and protein distributions below the classical ∼250-nm diffraction limit of light, but utilizing this technique effectively r...
In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes [0.03%]
用于检测一氧化氮的单壁碳纳米管的活体递送技术
Nicole M Iverson,Michael S Strano,Gerald N Wogan
Nicole M Iverson
Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a sm...