Methods to Validate Binding and Kinetics of "Proximity-Inducing" Covalent Immune-Recruiting Molecules [0.03%]
“邻近诱导”共价免疫募集分子的结合和亲和力验证方法学研究
Eden Kapcan,Benjamin Lake,Zi Yang et al.
Eden Kapcan et al.
The emergence of covalent inhibitors and chemoproteomic probes in translational chemical biology research requires the development of robust biophysical and analytical methods to characterize their complex interactions with target biomolecu...
Multiparametric High-Content Assays to Measure Cell Health and Oxidative Damage as a Model for Drug-Induced Liver Injury [0.03%]
用于测定细胞健康和氧化损伤的多参数高内涵分析作为药物性肝损伤模型
Grace Pohan,Jether Amos Espinosa,Steven Chen et al.
Grace Pohan et al.
Drug-induced liver injury is an important cause of non-approval in drug development and the withdrawal of already approved drugs from the market. Screening human hepatic cell lines for toxicity has been used extensively to predict drug-indu...
Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting [0.03%]
Northern印记法可视化RNA胞苷乙酰转移酶活性
Keri M Bryson,Supuni Thalalla-Gamage,Jordan L Meier
Keri M Bryson
Cytidine acetyltransferases are an emerging class of nucleic-acid-modifying enzymes responsible for the establishment of N4 -acetylcytidine (ac4C) in RNA. In contrast to histone acetyltransferases, whose activity is commonly studied by west...
Three-Color Imaging Enables Simultaneous Screening of Multiple RNA Targets on Small Molecule Microarrays [0.03%]
三色成像技术实现小分子微阵列上多种RNA靶点的同时检测
Deondre Jordan,Mo Yang,John S Schneekloth Jr
Deondre Jordan
Small molecule microarray (SMM) technology has become a powerful tool used in high-throughput screening for target-based drug discovery. One area in which SMMs have found use is the identification of small molecule ligands for RNA. RNAs wit...
Ziwen Jiang,Huan He,Hongxu Liu et al.
Ziwen Jiang et al.
Reversible addition-fragmentation chain-transfer (RAFT) polymerization is a commonly used polymerization methodology to generate synthetic polymers. The products of RAFT polymerization, i.e., RAFT polymers, have been widely employed in seve...
Suzanne E Dettling,Mina Ahmadi,Zongtao Lin et al.
Suzanne E Dettling et al.
Reverse-polarity activity-based protein profiling (RP-ABPP) is a chemical proteomics approach that uses nucleophilic probes amenable to "click" chemistry deployed into living cells in culture to capture, immunoprecipitate, and identify prot...
Caroline M Ganobis,M Sameer Al-Abdul-Wahid,Simone Renwick et al.
Caroline M Ganobis et al.
Metabolomic studies allow a deeper understanding of the processes of a given ecological community than nucleic acid-based surveys alone. In the case of the gut microbiota, a metabolic profile of, for example, a fecal sample provides details...
In Vitro Activity Assays to Quantitatively Assess the Endogenous Reversible Oxidation State of Protein Tyrosine Phosphatases in Cells [0.03%]
测定细胞中内源性酪氨酸磷酸酶可逆氧化状态的体外活性实验
Avinash D Londhe,Syed H M Rizvi,Benoit Boivin
Avinash D Londhe
The reversible oxidation of protein tyrosine phosphatases (PTPs) impairs their ability to dephosphorylate substrates in vivo. This transient inactivation of PTPs occurs as their conserved catalytic cysteine residue reacts with cellular oxid...
EndoVIPER-seq for Improved Detection of A-to-I Editing Sites in Cellular RNA [0.03%]
改进的内源性ADAR编辑检测方法EndoVIPER-seq
Steve D Knutson,Jennifer M Heemstra
Steve D Knutson
Adenosine to-inosine (A-to-I) RNA editing is a conserved post-transcriptional modification that is critical for a variety of cellular processes. A-to-I editing is widespread in nearly all types of RNA, directly imparting significant global ...
Metabolic Labeling for the Visualization and Identification of Potentially O-GlcNAc-Modified Proteins [0.03%]
代谢标记技术可视化及鉴定潜在O-GlcN酰化蛋白质
Nichole J Pedowitz,Balyn W Zaro,Matthew R Pratt
Nichole J Pedowitz
O-GlcNAcylation is a posttranslational modification involving the addition of the single monosaccharide N-acetylglucosamine (GlcNAc) onto serine and threonine residues of intracellular proteins. Though O-GlcNAc is found on ∼1000 proteins i...