Enriching s4 U-RNA Using Methane Thiosulfonate (MTS) Chemistry [0.03%]
用甲基噻磺酸盐(MTS)化学 enrichment s4 U-RNA
Erin E Duffy,Matthew D Simon
Erin E Duffy
Metabolic labeling of cellular RNA is a useful approach to study RNA biology. 4-Thiouridine (s4 U) is a convenient nucleoside for metabolic labeling because it is cell permeable and is incorporated into newly transcribed RNA, and the sulfur...
Nanoparticle-Templated Molecular Recognition Platforms for Detection of Biological Analytes [0.03%]
基于纳米颗粒的分子识别检测平台技术用于生物分析物检测
Abraham G Beyene,Gozde S Demirer,Markita P Landry
Abraham G Beyene
Molecular recognition of biological analytes with optical nanosensors provides both spatial and temporal biochemical information. A recently developed sensing platform exploits near-infrared fluorescent single-wall carbon nanotubes combined...
Nathan Moerke,Mohammad Fallahi-Sichani
Nathan Moerke
Reverse phase protein arrays (RPPAs), also called reverse phase lysate arrays (RPLAs), involve immobilizing cell or tissue lysates, in small spots, onto solid supports which are then probed with primary antibodies specific for proteins or p...
Fabrication of 3-D Reconstituted Organoid Arrays by DNA-Programmed Assembly of Cells (DPAC) [0.03%]
DNA程序化细胞组装技术构建三维类器官芯片数组
Michael E Todhunter,Robert J Weber,Justin Farlow et al.
Michael E Todhunter et al.
Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) composed into specific three-dimensional (3-D) structures. T...
Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS) [0.03%]
利用光诱导核定位信号（LINuS）在活细胞中进行光遗传学调控蛋白质输入到细胞核的方法
Pierre Wehler,Dominik Niopek,Roland Eils et al.
Pierre Wehler et al.
Many biological processes are regulated by the timely import of specific proteins into the nucleus. The ability to spatiotemporally control the nuclear import of proteins of interest therefore allows study of their role in a given biologica...
Combinatorial Library Screening Coupled to Mass Spectrometry to Identify Valuable Cyclic Peptides [0.03%]
结合质谱的组合文库筛选用于识别有价值的环状肽类化合物
Silvia A Camperi,Silvana L Giudicessi,María C Martínez-Ceron et al.
Silvia A Camperi et al.
Combinatorial library screening coupled to mass spectrometry (MS) analysis is a practical approach to identify useful peptides. Cyclic peptides can have high biological activity, selectivity, and affinity for target proteins, and high stabi...
Liquid Exfoliation of Layered Transition Metal Dichalcogenides for Biological Applications [0.03%]
液相剥离层状金属二硫化物用于生物医学应用
Emily P Nguyen,Torben Daeneke,Serge Zhuiykov et al.
Emily P Nguyen et al.
Known to possess distinctive properties that differ greatly from their bulk form, layered two-dimensional materials have been extensively studied and incorporated into many versatile applications ranging from optoelectronics to sensors. For...
Stephanie Mack,Munira F Fouz,Sourav K Dey et al.
Stephanie Mack et al.
Particularly for its use in bioconjugations, the copper-catalyzed (or copper-promoted) azide-alkyne cycloaddition (CuAAC) reaction or 'click chemistry', has become an essential component of the modern chemical biologist's toolbox. Click che...
Isotope Targeted Glycoproteomics (IsoTaG) to Characterize Intact, Metabolically Labeled Glycopeptides from Complex Proteomes [0.03%]
用于表征复杂蛋白质组中完整代谢标记糖肽的同位素靶向糖蛋白组学(IsoTaG)技术
Christina M Woo,Carolyn R Bertozzi
Christina M Woo
Protein glycosylation plays many critical roles in biological function and creates the most diversity of all post-translational modifications (PTMs). Glycan structural diversity is directly correlated with difficulty in characterizing the i...
Hollow-Fiber Methodology for Pharmacokinetic/Pharmacodynamic Studies of Antimalarial Compounds [0.03%]
空心纤维方法学在抗疟药的药代动力学/药效学研究中的应用
Emily Caton,Elizabeth Nenortas,Rahul P Bakshi et al.
Emily Caton et al.
Knowledge of pharmacokinetic/pharmacodynamic (PK/PD) relationships can enhance the speed and economy of drug development by enabling informed and rational decisions at every step, from lead selection to clinical dosing. For anti-infective a...