In Vitro Selection and Characterization of DNA Aptamers to a Small Molecule Target [0.03%]
体外筛选并表征针对小分子靶标的DNA适配体
Annamaria Ruscito,Erin M McConnell,Anna Koudrina et al.
Annamaria Ruscito et al.
Aptamers, synthetic oligonucleotide-based molecular recognition probes, have found use in a wide array of biosensing technologies based on their tight and highly selective binding to a variety of molecular targets. However, the inherent cha...
Robert W Lee,Mark Mitchnick
Robert W Lee
When a drug candidate-i.e., a new chemical entity (NCE) or new molecular entity (NME)-is discovered, there is a requirement to identify a vehicle for in vitro and/or in vivo evaluation to assess the activity and/or toxicity of the compound ...
Honghong Wu,Israel Santana,Joshua Dansie et al.
Honghong Wu et al.
Plant nanobiotechnology is an interdisciplinary field at the interface of nanotechnology and plant biology that aims to utilize nanomaterials as tools to study, augment or impart novel plant functions. The delivery of nanoparticles to plant...
The In Situ Enzymatic Screening (ISES) Approach to Reaction Discovery and Catalyst Identification [0.03%]
反应发现和催化剂识别的原位酶促筛选（ISES）方法
Robert A Swyka,David B Berkowitz
Robert A Swyka
The importance of discovering new chemical transformations and/or optimizing catalytic combinations has led to a flurry of activity in reaction screening. The in situ enzymatic screening (ISES) approach described here utilizes biological to...
Overview of Methods and Strategies for Conducting Virtual Small Molecule Screening [0.03%]
虚拟小分子筛选的方法与策略概述
Xavier Fradera,Kerim Babaoglu
Xavier Fradera
Virtual screening (VS) in the context of drug discovery is the use of computational methods to discover novel ligands with a desired biological activity from within a larger collection of molecules. These techniques have been in use for man...
A Guide to Quantitative Biomarker Assay Development using Whispering Gallery Mode Biosensors [0.03%]
基于倏逝波模式生物传感器的定量生物标志物检测试剂盒开发指南
Heather M Robison,Ryan C Bailey
Heather M Robison
Whispering gallery mode (WGM) sensors are a class of powerful analytical techniques defined by the measurement of changes in the local refractive index at or near the sensor surface. When functionalized with target-specific capture agents, ...
High-Throughput Screening of HECT E3 Ubiquitin Ligases Using UbFluor [0.03%]
基于UbFluor的HECT型E3泛素连接酶高通量筛选技术
Peter K Foote,David T Krist,Alexander V Statsyuk
Peter K Foote
HECT E3 ubiquitin ligases are responsible for many human disease phenotypes and are promising drug targets; however, screening assays for HECT E3 inhibitors are inherently complex, requiring upstream E1 and E2 enzymes as well as ubiquitin, ...
Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production [0.03%]
一种用于瞬时转染和慢病毒生产的优化PEI转染方法
Shaozhe Yang,Xiaoling Zhou,Rongxiang Li et al.
Shaozhe Yang et al.
Polyethyleneimine (PEI), a cationic polymer vehicle, forms a complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency ...
Characterizing the Nano-Bio Interface Using Microscopic Techniques: Imaging the Cell System is Just as Important as Imaging the Nanoparticle System [0.03%]
显微技术在纳米生物界面表征中的应用：细胞体系的成像和纳米粒子体系的一样重要
Christie M Sayes,Henry Lujan
Christie M Sayes
The rapid growth of nanotechnology and its industries has elevated the need to understand the risks associated with handling, using, and disposing of nanomaterials. These risks can be assessed through exposure measurement and hazard identif...
David B Beck,Roberto Bonasio
David B Beck
Identification of molecular interactions is paramount to understanding how cells function. Most available technologies rely on co-purification of a protein of interest and its binding partners. Therefore, they are limited in their ability t...