XNA Synthesis and Reverse Transcription by Engineered Thermophilic Polymerases [0.03%]
由工程嗜热聚合酶催化XNA合成与反转录
Christopher Cozens,Vitor B Pinheiro
Christopher Cozens
The B-family polymerases of hyperthermophilic archaea have proven an exceptional platform for engineering polymerases with extended substrate spectra, despite multiple mechanisms for detecting and avoiding incorporation of non-cognate subst...
Jayme L Dahlin,Matthew Cuellar,Gurpreet Singh et al.
Jayme L Dahlin et al.
Nonspecific target engagement by test compounds and purported chemical probes is a significant source of assay interference and promiscuous bioactivity in high-throughput screening (HTS) and chemical biology. Most counter-screens for thiol-...
Alexander I Taylor,Philipp Holliger
Alexander I Taylor
This unit describes the application of "synthetic genetics," i.e., the replication of xeno nucleic acids (XNAs), artificial analogs of DNA and RNA bearing alternative backbone or sugar congeners, to the directed evolution of synthetic oligo...
Mia L Huang,Ember M Tota,Stephen Verespy rd et al.
Mia L Huang et al.
This article describes a protocol for remodeling cells with synthetic glycoprotein and glycolipid mimetics that are functionalized with lipid anchors, allowing for cell surface display of specific glycan structures in predefined nanoscale a...
Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach [0.03%]
采用蛋白质肽谱库（ProPeL）方法表征蛋白激酶底物特异性
Joshua M Lubner,Jeremy L Balsbaugh,George M Church et al.
Joshua M Lubner et al.
Characterizing protein kinase substrate specificity motifs represents a powerful step in elucidating kinase-signaling cascades. The protocol described here uses a bacterial system to evaluate kinase specificity motifs in vivo, without the n...
Xenobiotic Nucleic Acid (XNA) Synthesis by Phi29 DNA Polymerase [0.03%]
Phi29 DNA聚合酶催化的外源核苷酸(XNA)的合成
Leticia L Torres,Vitor B Pinheiro
Leticia L Torres
Phi29 DNA polymerase (DNAP) is the replicative enzyme of the Bacillus subtilis bacteriophage Phi29. Its extraordinary processivity and its ability to perform isothermal amplification of DNA are central to many molecular biology applications...
Kevin Gorman,Jennifer McGinnis,Brian Kay
Kevin Gorman
Antibodies are useful tools for detecting individual proteins in complex samples and for learning about their location, amount, binding partners, and function in cells. Unfortunately, generating antibodies is time consuming and laborious, a...
Generating Conformation-Specific Polyclonal and Monoclonal Anti-Protein Kinase C Antibodies and Anti-Active State Specific PKC Antibodies [0.03%]
生成构象特异性多克隆及单克隆蛋白激酶C抗体和活性状态特异性PKC抗体
Darlene A Pena,Denise M V Pacheco,Paulo S L Oliveira et al.
Darlene A Pena et al.
The protein kinase C (PKC) family of serine/ threonine kinases has been shown to play active roles as either suppressors or promoters of carcinogenesis in different types of tumors. Using antibodies that preferentially recognize the active ...
Anton Iliuk
Anton Iliuk
Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has enabled researchers to analyze complex biological samples with unprecedented depth. It facilitates the identification and quantification of modifications within th...
Michiko Kimoto,Ken-Ichiro Matsunaga,Ichiro Hirao
Michiko Kimoto
A novel technology, genetic alphabet expansion, has rapidly advanced through the successful creation of unnatural base pairs that function as a third base pair in replication. Recently, genetic alphabet expansion has been applied to some pr...