Endotoxin-Free Preparation of Graphene Oxide and Graphene-Based Materials for Biological Applications [0.03%]
用于生物应用的无内毒素石墨烯氧化物和石墨烯基材料的制备
Dorsa Parviz,Michael Strano
Dorsa Parviz
Due to their two-dimensional structure and unique properties, graphene and its derivatives have been extensively studied for their potential applications in various fields ranging from electronics to composites. Particularly, their high sur...
Mena Asha Krishnan,Amit Pandit,Venkatesh Chelvam
Mena Asha Krishnan
The development of small molecule ligand-targeted therapeutics is currently of paramount importance for treatment of cancer due to their potential to reduce system toxicity and increase potency of a delivered chemotherapeutic drug. The main...
Preparation of Ligand-Targeted Drug Conjugates for Cancer Therapy and Their Evaluation In Vitro [0.03%]
用于癌症治疗的配体靶向药物缀合物的制备及体外评价
Mena Asha Krishnan,Sagnik Sengupta,Venkatesh Chelvam
Mena Asha Krishnan
Present treatment strategies focus on minimizing unwanted toxicity to healthy cells during chemotherapeutic treatment. This is achieved by developing strategies to selectively deliver drugs to malignant cells over-expressing specific protei...
Single-Protein-Specific Redox Targeting in Live Mammalian Cells and C. elegans [0.03%]
活细胞和线虫中蛋白质特异的氧化还原修饰技术
Alexandra Van Hall-Beauvais,Yi Zhao,Daniel A Urul et al.
Alexandra Van Hall-Beauvais et al.
T-REX (targetable reactive electrophiles and oxidants) enables electrophile targeting in living systems with high spatiotemporal precision and at single-protein-target resolution. T-REX allows functional consequences of individual electroph...
Monitoring PARKIN RBR Ubiquitin Ligase Activation States with UbFluor [0.03%]
利用UbFluor监测PARKIN RBR泛素连接酶的激活状态
Peter K Foote,Alexander V Statsyuk
Peter K Foote
PARKIN is a RING-Between-RING (RBR) E3 ligase, which ubiquitinates mitochondrial proteins in response to mitochondrial damage. Ser65 of PARKIN is phosphorylated by kinase PINK1 (pPARKIN), which causes partial PARKIN activation. PINK1 also p...
Metabolic Labeling of Prenylated Proteins Using Alkyne-Modified Isoprenoid Analogues [0.03%]
使用带烷基叠氮的异戊二烯类似物对焦磷酸法尼酯标记蛋白质的研究
Kiall F Suazo,Alexander K Hurben,Kevin Liu et al.
Kiall F Suazo et al.
Protein prenylation involves the attachment of a farnesyl or geranylgeranyl group onto a cysteine residue located near the C-terminus of a protein, recognized via a specific prenylation motif, and results in the formation of a thioether bon...
Design and Functional Characterization of Synthetic E3 Ubiquitin Ligases for Targeted Protein Depletion [0.03%]
用于靶蛋白耗竭的合成E3泛素连接酶的设计与功能表征
Morgan R Baltz,Erin A Stephens,Matthew P DeLisa
Morgan R Baltz
A number of techniques now exist for decreasing the expression of cellular proteins without the need for genomic modification. One such technique involves engineered protein chimeras that combine the ubiquitination activity of E3 ubiquitin ...
Moritz Welter,Andreas Marx
Moritz Welter
In this unit the preparation and application of enzyme-nucleotide conjugates is depicted. First, a modified nucleoside triphosphate is synthesized bearing a long and flexible linker equipped with a thiol group. The nucleotide is then reacte...
Zhanar Abil,Andrew D Ellington
Zhanar Abil
Compartmentalized self-replication (CSR) is an emulsion PCR-based method for the selection of DNA polymerases. E. coli host cells expressing a library of DNA polymerases are emulsified so that no more than a single cell is present in a sing...
Elena Eremeeva,Piet Herdewijn
Elena Eremeeva
An efficient PCR amplification of various templates (short 57-mer, random 67- and 82-mer, and long DNA) with base-modified nucleoside triphosphates is presented here. Using 5-substituted pyrimidine and 7-substituted-7-deaza- or 8-substitute...